烟草C2H2锌指蛋白转录因子家族成员的鉴定与表达分析

doi:10.16288/j.yczz.15-440

遗传 ›› 2016, Vol. 38 ›› Issue (4): 337-349.doi: 10.16288/j.yczz.15-440

烟草C2H2锌指蛋白转录因子家族成员的鉴定与表达分析

杨明磊^{1, 2}, 晁江涛¹, 王大伟¹, 胡军华^{1, 2}, 吴华^{1, 2}, 龚达平¹, 刘贯山¹

1. 中国农业科学院烟草研究所,中国农业科学院烟草遗传改良与生物技术重点开放实验室,青岛 266101;
2. 中国农业科学院研究生院,北京 100081

收稿日期:2015-10-21 修回日期:2015-12-25 出版日期:2016-04-20 发布日期:2016-04-20
通讯作者: 刘贯山,研究员,博士生导师,研究方向：烟草分子育种。E-mail: liuguanshan@caas.cn
作者简介:杨明磊,在读硕士,专业方向：烟草突变体鉴定与利用。E-mail: 787093482@qq.com晁江涛,助理研究员,研究方向：生物信息学。E-mail: 290594533@qq.com杨明磊和晁江涛为并列第一作者。
基金资助:
国家烟草专卖局烟草基因组计划重大专项项目(编号：110201301005(JY-05))和中国农业科学院烟草研究所青年科学基金项目(编号：2015B05)[Supported by the Tobacco Genome Project of State Tobacco Monopoly Administration(No.110201301005(JY-05)) and the Science Foundation for Young Scholars of Institute of Tobacco Research of CAAS(No; 2015B05)]

Genome-wide identification and expression profiling of the C2H2-type zinc finger protein transcription factor family in tobacco

Minglei Yang^{1, 2}, Jiangtao Chao¹, Dawei Wang¹, Junhua Hu^{1, 2}, Hua Wu^{1, 2}, Daping Gong¹, Guanshan Liu¹

1. Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute of Chinese Academy of Agricultural Science, Qingdao 266101, China;
2. Graduate School of Chinese Academy of Agricultural Science, Beijing 100081, Chinbr/a

Received:2015-10-21 Revised:2015-12-25 Online:2016-04-20 Published:2016-04-20

摘要/Abstract

摘要： C2H2锌指蛋白转录因子家族在真核生物中具有重要的生物学功能,广泛参与植物叶的发生、花器官的调控、侧枝的形成及逆境胁迫等生命过程。植物C2H2锌指蛋白不仅结合DNA和RNA,而且与蛋白质之间相互作用。本研究利用普通烟草(Nicotiana tabacum)基因组数据库,运用Blastp比对,结合Pfam和SMART分析,鉴定了118条普通烟草C2H2锌指蛋白家族成员;对烟草C2H2锌指蛋白家族进行了进化树分析、结构域分析、物理化学性质分析、染色体定位、基因结构分析、三维结构分析及组织表达分析等。结果表明：不同成员的氨基酸长度差异较大;系统进化及结构域分析显示,所有C2H2家族成员可以被分为5个亚家族,同一亚家族成员之间在结构域和理化性质上呈现较高一致性;每个成员都含有C2H2结构域,在数量上存在较大差异;将所有基因家族成员定位在22条染色体上;组织表达分析表明,每个C2H2亚家族都有成员在不同组织中表达,在叶及根中有些基因的表达量较高。

关键词: C2H2锌指蛋白转录因子, 烟草, 基因家族

Abstract: C2H2 zinc finger protein transcription factor family members have important biological functions in eukaryotes. They not only bind DNA and RNA, but also interact with proteins. In this study, 118 members of the tobacco C2H2 zinc finger protein transcription factor family were identified from the N. tabacum genome database by using Pfam, SMART and Blastp. The analyses of phylogenetic tree, physical and chemical properties, chromosomal mapping, gene structures, protein three-dimensional structures and tissue expression patterns were performed. The results suggested that the peptide length of different subfamily members is significantly different. Phylogenetic and motif analysis revealed that the C2H2 zinc finger protein transcription factor family members can be divided into 5 subfamilies and each member has at least one C2H2 motif. Genes of the family members are distributed across the 22 chromosomes. C2H2 zinc finger protein transcription factor family members are expressed in different tissues although some have higher expression levels in leaves and roots. This study will be helpful for further analysis of the C2H2 zinc finger family proteins in other plants.

Key words: C2H2 zinc finger protein transcription factor, tobacco, gene family

杨明磊, 晁江涛, 王大伟, 胡军华, 吴华, 龚达平, 刘贯山. 烟草C2H2锌指蛋白转录因子家族成员的鉴定与表达分析[J]. 遗传, 2016, 38(4): 337-349.

Minglei Yang, Jiangtao Chao, Dawei Wang, Junhua Hu, Hua Wu, Daping Gong, Guanshan Liu. Genome-wide identification and expression profiling of the C2H2-type zinc finger protein transcription factor family in tobacco[J]. HEREDITAS(Beijing), 2016, 38(4): 337-349.

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烟草C2H2锌指蛋白转录因子家族成员的鉴定与表达分析

Genome-wide identification and expression profiling of the C2H2-type zinc finger protein transcription factor family in tobacco

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