葡萄生长素响应基因家族生物信息学鉴定和表达分析

doi:10.16288/j.yczz.14-390

遗传 ›› 2015, Vol. 37 ›› Issue (7): 720-730.doi: 10.16288/j.yczz.14-390

• 研究报告 • 上一篇

葡萄生长素响应基因家族生物信息学鉴定和表达分析

袁华招, 赵密珍, 吴伟民, 于红梅, 钱亚明, 王壮伟, 王西成

江苏省农业科学院园艺研究所/江苏省高效园艺作物遗传改良重点实验室,南京 210014

收稿日期:2014-11-10 修回日期:2014-12-08 出版日期:2015-07-20 发布日期:2015-07-20
通讯作者: 赵密珍,研究员, 硕士生导师,研究方向：果树学。E-mail：njzhaomz@163.com
作者简介:袁华招,硕士,专业方向：果树学。E-mail: 544250348@qq.com
基金资助:
江苏省农业科技自主创新资金项目(编号：CX(12) 2013)资助

Genome-wide identification and expression analysis of auxin-related gene families in grape

Huazhao Yuan, Mizhen Zhao, Weimin Wu, HongMei Yu, Yaming Qian, Zhuangwei Wang, Xicheng Wang

Institute of Horticulture, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China

Received:2014-11-10 Revised:2014-12-08 Online:2015-07-20 Published:2015-07-20

摘要/Abstract

摘要： 生长素响应基因家族能调节植物体内生长素平衡和生长素信号途径。文章采用生物信息学方法检索获得葡萄(Vitisvinifera L.)基因组数据库中的生长素响应基因,通过分析其染色体定位、基因共线性和系统进化,发现葡萄基因组含有25个AUX_IAA基因、19个ARF基因、9个GH3基因、42个LBD基因。这些生长素响应基因不均匀分布在葡萄的19条染色体上,部分家族基因在染色体上形成基因簇。葡萄芯片数据结果表明,生长素响应基因在葡萄不同时期的果实和叶芽中均有表达,尤其在果实转色期、叶芽萌发或休眠期表达量急剧变化。研究结果为葡萄生长素响应基因在叶片和果实发育过程中的功能研究提供参考。

关键词: 葡萄, 生长素响应基因家族, 进化, 果实发育

Abstract: The auxin response gene family adjusts the auxin balance and the growth hormone signaling pathways in plants. Using bioinformatics methods, the auxin-response genes from the grape genome database are identified and their chromosomal location, gene collinearity and phylogenetic analysis are performed. Probable genes include 25 AUX_IAA, 19 ARF, 9 GH3 and 42 LBD genes, which are unevenly distributed on all 19 chromosomes and some of them formed distinct tandem duplicate gene clusters. The available grape microarray databases show that all of the auxin-response genes are expressed in fruit and leaf buds, and significant overexpressed during fruit color-changing, bud break and bud dormancy periods. This paper provides a resource for functional studies of auxin-response genes in grape leaf and fruit development.

Key words: grape, auxin-response gene family, phylogeny, fruit development

袁华招, 赵密珍, 吴伟民, 于红梅, 钱亚明, 王壮伟, 王西成. 葡萄生长素响应基因家族生物信息学鉴定和表达分析[J]. 遗传, 2015, 37(7): 720-730.

Huazhao Yuan, Mizhen Zhao, Weimin Wu, HongMei Yu, Yaming Qian, Zhuangwei Wang, Xicheng Wang. Genome-wide identification and expression analysis of auxin-related gene families in grape[J]. HEREDITAS(Beijing), 2015, 37(7): 720-730.

参考文献

[1] Davies PJ. Plant hormones: physiology, biotechnology and molecular biology. London: Kluwer Academic Publishers, 2004.
[2] Given NK, Venis MA, Grierson D. Hormonal regulation of ripening in the strawberry, a non-climacteric fruit. Planta , 1988, 174(3): 402-406.
[3] Chapman EJ, Estelle M. Mechanism of auxin-regulated gene expression in plants. Annu Rev Genet , 2009, 43(11): 265-285.
[4] Guilfoyle TJ, Ulmasov T, Hagen G. The ARF family of transcription factors and their role in plant hormone-responsive transcription. Cell Mol Life Sci , 1998, 54(7): 619-627.
[5] Gray WM, Kepinski S, Rouse D, Leyser O, Estelle M. Auxin regulates SCFTIR1-dependent degradation of AUX/IAA proteins. Nature , 2001, 414(6861): 271-276.
[6] Abel S, Theologis A. Early genes and auxin action. Plant Physiol, 1996, 111: 9-17.
[7] Kim J, Harter K, Theologis A. Protein-protein interactions among the Aux/IAA proteins. Proc Natl Acad Sci USA, 1997, 94(22): 11786-11791.
[8] Morgan KE, Zarembinski TI, Theologis A, Abel S. Biochemical characterization of recombinant polypeptides corresponding to the predicted βααfold in Aux/IAA proteins. FEBS Lett , 1999, 454(3): 283-287.
[9] Ouellet F, Overvoorde PJ, Theologis A. IAA17/AXR3: biochemical insight into an auxin mutant phenotype. Plant Cell , 2001, 13(4): 829-841.
[10] Ulmasov T, Hagen G, Guilfoyle TJ. ARF1, a transcription factor that binds to auxin responseelements. Science , 1997, 276(5320): 1865-1868.
[11] Ulmasov T, Hagen G, Guilfoyle TJ. Activation and repression of transcription by auxin-responsefactors. Proc Natl Acad Sci USA , 1999, 96(10): 5844-5849.
[12] Ulmasov T, Murfett J, Hagen G, Guilfoyle TJ. Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. Plant Cell , 1997, 9(11): 1963-1971.
[13] de Jong M, Wolters-Arts M, Feron R, Mariani C, Vriezen WH. The Solanum lycopersicum auxin response factor 7 ( Sl ARF7) regulates auxin signaling during tomato fruit set and development. Plant J , 2009, 57(1): 160-170.
[14] Wang H, Jones B, Li ZG, Frasse P, Delalande C, Regad F, Chaabouni S, Latche A, Pech JC, Bouzayen M. The tomato Aux / IAA transcription factor IAA 9 is involved in fruit development and leaf morphogenesis. Plant Cell , 2005, 17(10): 2676-2692.
[15] Guillon F, Philippe S, Bouchet B, Devaux MF, Frasse P, Jones B, Bouzayen M, Lahaye M. Down-regulation of an Auxin response factor in the tomato induces modification of fine pectin structure and tissue architecture. J Exp Bot , 2008, 59(2): 273-288.
[16] Jones B, Frasse P, Olmos E, Zegzouti H, Li ZG, Latché A, Pech JC, Bouzayen M. Down-regulation of DR12, an auxin-response-factor homolog, in the tomato results in a pleiotropic phenotype including dark green and blotchy ripening fruit. Plant J , 2002, 32(4): 603-613.
[17] Staswick PE, Tiryaki I, Rowe ML. Jasmonate response locus JAR1 and several related Arabidopsis genes encode enzymes of the firefly luciferase superfamily that show activity on jasmonic, salicylic, and indole-3-acetic acids in an assay for adenylation. Plant Cell , 2002, 14(6): 1405-1415.
[18] Staswick PE, Serban B, Rowe M, Tiryaki I, Maldonado MT, Maldonado MC, Suzaa W. Characterization of an Arabidopsis enzyme family that conjugates amino acids to Indole-3-Acetic Acid. Plant Cell , 2005, 17(2): 616-627.
[19] Riemann M, Riemann M, Takano M. Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling. Plant Cell Environ , 2008, 31(6): 783-792.
[20] Zhang SW, Li CH, Cao J, Zhang YC, Zhang SQ, Xia YF, Sun DY, Sun Y. Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3. 13 activation. Plant Physiol , 2009, 151(4): 1889-1901.
[21] Staswick PE, Tiryaki I. The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in Arabidopsis . Plant Cell , 200

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葡萄生长素响应基因家族生物信息学鉴定和表达分析

Genome-wide identification and expression analysis of auxin-related gene families in grape

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