遗传 ›› 2006, Vol. 28 ›› Issue (7): 805-809.

• 研究报告 • 上一篇    下一篇

MTNR1A基因对大白猪和长白猪产仔数的影响

王晓凤1,2,王爱国1,2,傅金恋1,2,李宗进3,杨淑明4,韩丽萍4   

  1. 1. 中国农业大学动物科技学院,农业部畜禽遗传育种重点开放实验室,北京100094;2. 中国农业大学农业生物技术国家重点实验室,北京100094;3. 河北省青龙县畜牧局,河北青龙066500;4.北京市顺义区畜牧服务中心,北京101300

  • 收稿日期:2005-10-12 修回日期:2005-12-30 出版日期:2006-07-10 发布日期:2006-07-10
  • 通讯作者:

    王爱国

Effects of MTNR1A gene on litter size in a Large White and a Landrace Herd

WANG Xiao-Feng1,2,WANG Ai-Guo1,2 ,FU Jin-Lian1,2,LI Zong-Jin3,YANG Shu-Ming4,HAN Li-Ping4

  

  1. 1. The Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing100094,China;2.State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing100094,China;3.The Bureau of Husbandry,Qinglong Hebei 066500,China;4.Beijing Husbandry Service Centre, Shunyi Beijing101300,China

  • Received:2005-10-12 Revised:2005-12-30 Online:2006-07-10 Published:2006-07-10
  • Contact:

    WANG Ai-Guo

摘要: 根据MTNR1A基因在GenBank中的已知DNA序列设计了2对引物,采用PCR-SSCP技术在一个大白猪和长白猪群体中进行单核苷酸多态性(single nucleotide polymorphism, SNP)检测,发现了一个SNP位点,并对其不同基因型个体PCR回收产物进行测序。测序结果发现该SNP是由于在+159碱基处(GenBank中序列)发生了G→A的同义突变而引起的。并对该SNP与产仔数进行了关联分析,结果表明该SNP对产仔数有影响。

关键词: MTNR1A, 单核苷酸多态性, 猪, 产仔数

Abstract: In this study, two pairs of primers were designed based on the known sequence for DNA in GenBank for amplification of genomic DNA in a Large White and a Landrace herd. The PCR products were detected by PCR-SSCP, we found that the products from the first pair of primer exist a SNP, and PCR products from randomly selected different genotypes were sequenced after were recalled and purified, the result disclosed that the single base mutation(G→A) occurred at +159bp(in terms of sequence situation in Genbank) for the BB genotype, which were a synonymous mutation. And analyzed association of this mutation with litter size traits, the results found that this mutation of MTNR1A gene had some affect on litter size traits.

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