遗传 ›› 2021, Vol. 43 ›› Issue (12): 1170-1178.doi: 10.16288/j.yczz.21-335
韩程程1,2(), 夏凯1,3, 龚茹莹1, 吴栩涵1, 张蕾1,2(
), 梁新乐1,2(
)
收稿日期:
2021-09-21
修回日期:
2021-11-30
出版日期:
2021-12-20
发布日期:
2021-12-10
通讯作者:
张蕾,梁新乐
E-mail:707884766@qq.com;zhanglei@zjsu.edu.cn;dbiot@mail.zjgsu.edu.cn
作者简介:
韩程程,博士研究生,研究方向:食品生物技术。E-mail:
Han Chengcheng1,2(), Xia Kai1,3, Gong Ruying1, Wu Xuhan1, Zhang Lei1,2(
), Liang Xinle1,2(
)
Received:
2021-09-21
Revised:
2021-11-30
Online:
2021-12-20
Published:
2021-12-10
Contact:
Zhang Lei,Liang Xinle
E-mail:707884766@qq.com;zhanglei@zjsu.edu.cn;dbiot@mail.zjgsu.edu.cn
摘要:
非洲猪瘟病毒(African swine fever virus, ASFV)是近年来流行的一种主要致病病毒,对我国的国民经济生活造成了严重影响。本研究以非洲猪瘟病毒的编码保守蛋白p54基因序列片段为检测目标,结合点亮 Spinach RNA适配体的开关功能,设计了Spinach-p54的嵌合式RNA适配体。其特异地结合目标序列并产生荧光,实现了在RNA水平上对非洲猪瘟病毒的快速检测。研究结果表明,核酸适配体浓度200 nmol/L,反应温度为37 ℃,该方法检出限为200 nmol/L,线性范围为200~400 nmol/L。该方法特异性强,操作简单,灵敏度高等特点,可用于市场和养殖场的现场快速检测。
韩程程, 夏凯, 龚茹莹, 吴栩涵, 张蕾, 梁新乐. 适于检测非洲猪瘟病毒的点亮Spinach-p54 RNA适配体的设计及应用[J]. 遗传, 2021, 43(12): 1170-1178.
Han Chengcheng, Xia Kai, Gong Ruying, Wu Xuhan, Zhang Lei, Liang Xinle. Design and application of lightening Spinach-p54 RNA apatmer to detect African swine fever virus[J]. Hereditas(Beijing), 2021, 43(12): 1170-1178.
表1
适配体名称和序列"
适配体名称 | 序列(5′→3′) |
---|---|
Spinach-p54-1 | GATGCCGCGGATGGCGAAGGACGGGTCCAGTGCGTTCGCGCACTGTTGAGTAGAGTGTGAGCGCCAAACCGGTTGA |
Spinach-p54-2 | ACTGTCGTGTATGGCGAAGGACGGGTCCAGTGCGTTCGCGCACTGTTGAGTAGAGTGTGAGCGCCAGGCTCAGTCG |
Spinach-p54-3 | CCATAATGCCGTGGCGAAGGACGGGTCCAGTGCGTTCGCGCACTGTTGAGTAGAGTGTGAGCGCCCGGATAAACCG |
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