关键词: 表达, 克隆, 催乳素基因, 白鹅

Abstract: Abstract: The mature segment gene of prolactin (PRL) in White Goose was amplified from pituitary by RT-PCR and then cloned into the pMD18-T vector. Sequencing analysis showed that the cDNA has a length of 690 bp including the termina-tion codon and encodes a protein composed of 230 amino acids, which differs from the published PRL cDNA sequence. There is a homology of 99.57% in base and 99.56% in amino acids with that of Wanxi White Goose, respectively. A pro-karyotic expression vector, pET-32a(+), was used to construct the recombinant plasmid pET-32a(+)-PRL to produce protein. Having been induced by IPTG，the host cell carrying the recombinant plasmid expressed the recombinant PRL. The opti-mal condition for expression is 1 mmol/L IPTG at 37℃. Based on this condition, the expression rose to the highest level by 4 h of induction, accounting for 28.96% of the total bacterial protein.