遗传 ›› 2008, Vol. 30 ›› Issue (12): 1545-1549.doi: 10.3724/SP.J.1005.2008.01545

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microRNA在胚胎干细胞中的表达及作用

王世华; 边春景; 赵春华

  

  1. 中国医学科学院中国协和医科大学基础所组织工程中心, 北京 100005

  • 收稿日期:2008-05-16 修回日期:2008-07-08 出版日期:2008-12-10 发布日期:2008-12-10
  • 通讯作者: 赵春华

Expression and function of microRNA in embryonic stem cell

WANG Shi-Hua; BIAN Chun-Jing; ZHAO Chun-Hua

  

  1. Center of Tissue Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China

  • Received:2008-05-16 Revised:2008-07-08 Online:2008-12-10 Published:2008-12-10
  • Contact: ZHAO Chun-Hua

摘要:

摘要: 胚胎干细胞是一类具有自我更新能力和多向分化潜能的细胞, 其自我更新和多向分化过程都在遗传和表观遗传的严格调控下进行的。越来越多的研究表明microRNA 也在这一过程中发挥重要的作用。microRNA是一类内源性的非编码RNA, 能够通过与靶mRNA特异性的结合而导致靶mRNA降解或抑制其翻译, 从而对基因进行转录后调控。文章就microRNA在胚胎干细胞中的表达及其作用的研究进展做一综述。主要讨论一些在胚胎干细胞中特异性表达的microRNA, 以及这些microRNA 对胚胎干细胞自我更新和未分化状态的维持和继续分化增殖的调控作用。

关键词: 胚胎干细胞, microRNA, 分化

Abstract:

Abstract: Embryonic stem cells are the cells that have the ability both to self-renew and to differentiate into all the mature cell types in an adult. Both of these processes are tightly regulated by genetic and epigenetic mechanisms. Increasing evi-dence indicates that a class of single-stranded non-coding RNA, known as “microRNAs”, also plays a critical role in this process. MicroRNA can bind to target mRNAs by specific base pairing, then degrade mRNAs or inhibit protein translation. Therefore, they can participate in post-transcriptiona1 regulation. Recently, scientists began to study the effect of microRNA on embryonic stem cell and found that some microRNAs are specifically expressed and form an intricate network of mi-croRNAs, regulating key transcription factors and other genes. This review focuses on the expression of microRNA in em-bryonic stem cell and their functions. We discuss some microRNA that are specifically expressed in embryonic stem cell and their regulating effect on self-renewal and differentiation.