遗传 ›› 2011, Vol. 33 ›› Issue (1): 40-47.doi: 10.3724/SP.J.1005.2011.00040

• 综述 • 上一篇    下一篇

基因工程培育可恢复的植物雄性不育系的研究进展

王玉锋1,2, 黄霁月1, 杨金水1   

  1. 1. 复旦大学生命科学学院遗传工程国家重点实验室, 上海 200433; 
    2. 中国科学院上海生命科学研究院植物生理生态所, 上海 200032
  • 收稿日期:2010-04-22 修回日期:2010-05-06 出版日期:2011-01-20 发布日期:2010-01-20
  • 通讯作者: 杨金水 E-mail:jsyang@fudan.edu.cn
  • 基金资助:

    国家重大基础研究规划项目(973)(编号: 2007CB109002)资助

Progress in the study of producing reversible male sterile line by ge-netic engineering

WANG Yu-Feng1,2, HUANG Ji-Yue1, YANG Jin-Shui1   

  1. 1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China
    2. Institute of Plant Physiology and Ecology, Shanghai institutes for biological sciences, Chinese Academy of Sciences, Shanghai 200032, China
  • Received:2010-04-22 Revised:2010-05-06 Online:2011-01-20 Published:2010-01-20
  • Contact: YANG Jin-Shui E-mail:jsyang@fudan.edu.cn

摘要: 植物雄性不育是植物杂种优势利用的资源, 具有重要的生产利用价值。植物雄性不育可从自然突变、人工诱变和远缘杂交中发现, 现在可通过细胞工程和基因工程等方法来创造。文章综述了利用基因工程方法制备雄性不育品系及其相应的育性恢复策略, 分为“单组分策略”和“双组分策略”。其中利用“单组分策略”制备的不育植株是条件型雄性不育(可逆转的雄性不育), 它能在特定的条件下实现雄性可育与不育的转换, 实践中可直接作为两用系(不育系和保持系)用于两系法杂交制种; “双组分策略”是利用基因互作和亲本杂交直接培育雄性不育系, 或利用基因互作原理分别研制不育系和恢复系, 用于三系法生产杂交种。文章分析了 “单组分策略”和“双组分策略”的基因工程方法培育雄性不育系及其相应育性恢复策略优缺点, 对以上两种技术路线在实际应用中的现状作了分析和展望。

关键词: 雄性不育, 基因工程, 育性恢复, 条件型/可逆转的雄性不育

Abstract: Plant male sterility is a kind of resource of heterosis, which has important value in production. It may be derived from natural mutations, artificial mutations, distant hybridizations, and now through cell engineering and genetic engineering. This paper reviews the progress of strategies in production of plant male sterile lines and their corresponding fertile lines via genetic engineering approach. All strategies can be grouped into “single component strategy” and “two-component strategy”. “Single component strategy” produces conditional (reversible) male sterile line, whose fertility can be switched under given condition. Conditional male sterile line has two roles, which are CMS and maintainer line for breeding in practice; “two-component strategy” takes advantage of gene interaction and parental hybridization to generate male sterile line. Otherwise, it develops sterile line and restorer line respectively for three-line hybrid system for seed production through gene interaction. This paper discusses the advantages and disadvantages of gene engineering approaches of “single component strategy” and “two-component strategy” for developing male sterile line and corresponding restorer line, as well as the current status and perspective of these approaches in practice.

Key words: male sterility, genetic engineering, conditional /reversible male sterility, restoring fertility