遗传 ›› 2012, Vol. 34 ›› Issue (3): 335-341.doi: 10.3724/SP.J.1005.2012.00335

• 研究报告 • 上一篇    下一篇

小鼠不同泌乳期乳腺脂肪合成相关基因的mRNA表达

韩立强1, 李宏基1, 王月影1, 王林枫1, 杨国庆1, 王艳玲2, 杨国宇2   

  1. 1. 河南农业大学牧医工程学院, 郑州 450002 2. 河南农业大学农业部动物生化与营养重点实验室, 郑州 450002
  • 收稿日期:2011-05-19 修回日期:2011-06-03 出版日期:2012-03-20 发布日期:2012-03-25
  • 通讯作者: 杨国宇 E-mail:haubiochem@163.com
  • 基金资助:

    国家重点基础研究发展规划项目(973计划)(编号:2011CB100802)资助

mRNA expression of lipogenic genes in mouse mammary gland in different lactation stages

HAN Li-Qiang1, LI Hong-Ji1, WANG Yue-Ying1, WANG Lin-Feng1, YANG Guo-Qing1, WANG Yan-Ling2, YANG Guo-Yu2   

  1. 1. College of Animal Husbandry and Veterinary Science, Henan Agriculture University, Zhengzhou 450002, China 2. Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2011-05-19 Revised:2011-06-03 Online:2012-03-20 Published:2012-03-25

摘要: 为了研究小鼠不同泌乳期乳脂肪合成相关基因的表达规律, 文章采用荧光定量PCR检测了小鼠乳腺中与脂肪合成和分泌相关20个基因的mRNA相对表达丰度和表达差异。结果表明, 在乳腺中脂蛋白脂酶(LPL)、乙酰辅酶A羧化酶(ACACA)、硬脂酰辅酶A去饱和酶(SCD)、黄嘌呤脱氢酶(XDH)、嗜乳脂蛋白(BTN)、脂肪酸分化蛋白(ADFP)基因都具有高mRNA表达丰度 (表达丰度>5%), 脂肪酸转运体(CD36)、脂肪酸合成酶(FASN)、1-酰基甘油磷酸酰基转移酶(AGPAT6)和甘油酰基转移酶(DGAT)基因具有中等mRNA表达丰度(5%>表达丰度>1%), 与妊娠期乳腺基因的mRNA表达相比, 在泌乳期这些基因的mRNA表达均有显著上调(P<0.05), 并且ACACA、SCD、FASN、AGPAT6DGAT等脂肪合成酶基因的表达在泌乳中期(12 d)最高, 而在泌乳初期(6 d)和泌乳末期(18 d)较低, 呈现低-高-低的表达模式。转录因子固醇调节元件结合蛋白(SREBF)基因在泌乳开始时mRNA表达增加, 在泌乳中期(12 d)表达有10倍上调, 其变化规律与脂肪合成酶基因的表达模式相同, 说明SREBF基因在小鼠乳腺脂肪合成酶基因的表达调控中发挥重要调节作用。

关键词: 脂肪合成, 乳腺组织, 基因表达, 固醇调节元件结合蛋白

Abstract: To investigate the expression pattern of lipogenic genes in mammary gland of mouse in different lactation stages, the relative mRNA abundance and expression of 20 genes involved in milk fat synthesis and secretion of lactating mammary tissues were assessed using real-time PCR. Results revealed the significant upregulation of mRNA expression for both high abundant genes (abundance > 5%), such as LPL, ACACA, SCD, XDH, BTN, and ADFP and moderate abundant genes (5% > abundance > 1%), such as CD36, FASN, AGPAT6, and DGAT in lactation stages compared to pregnancy (P < 0.05). The mRNA expression of lipogenic enzyme genes for ACACA, SCD, FASN, AGPAT6, and DGAT exhibited lower expression lever in early (6 d) and late (18 d) lactation stages but higher expression level at middle stage (12 d), demonstrating a low-high-low pattern. Besides, the mRNA levels of some gene regulators were also measured. The mRNA expression of SREBF gene increased gradually along with the lactation, which showed a 10-fold elevation at middle stage (12 d). The expression pattern of SREBF gene was the same as lipogenic enzyme genes, suggesting that SREBF may play an important role in the regulation of lipogenic enzyme genes in the lactating mammary gland.

Key words: lipid synthesis, mammary tissue, gene expression, SREBF