天然嵌合基因的结构特性及其对基因设计的启示

doi:10.16288/j.yczz.17-268

遗传 ›› 2018, Vol. 40 ›› Issue (2): 135-144.doi: 10.16288/j.yczz.17-268

天然嵌合基因的结构特性及其对基因设计的启示

李迎侠(),张婷婷(),马磊()

石河子大学生命科学学院，石河子 832000

收稿日期:2017-08-08 修回日期:2017-12-22 出版日期:2018-02-20 发布日期:2017-12-25
作者简介:作者简介: 李迎侠,硕士研究生,专业方向：遗传学。E-mail: lyx1991a2a2@163.com|张婷婷,硕士,讲师,研究方向：分子遗传学。E-mail: zting@shzu.edu.cn|通讯作者: 马磊,博士,副教授,研究方向：生物信息学与分子遗传学。E-mail: malei1979@hotmail.com
基金资助:
国家自然科学基金(31272416,31560310);人社部留学人员科技活动择优资助[Supported by(31272416,31560310)

Structural characteristics of natural chimeric genes and their implications for gene design

Li Yingxia(),Zhang Tingting(),Ma Lei()

College of Life Science, Shihezi University, Shihezi 832000, China

Received:2017-08-08 Revised:2017-12-22 Online:2018-02-20 Published:2017-12-25
Supported by:
the National Natural Science Foundation of China(31272416,31560310);Scientific Research Foundation of the MHRSS of China for the Returned Overseas Chinese Scholars(31272416,31560310)

摘要/Abstract

摘要：

天然嵌合基因(natural chimeric gene)是由两个或两个以上的独立基因天然融合而成的新基因,该类型基因的发现,突破了“一个基因对应一个染色体座位”的经典认知,扩展了基因的概念。在人类癌症研究过程中,诸多的嵌合基因可导致肿瘤相关疾病,并作为癌症分子的诊断标志而受到人们的广泛关注。本文基于嵌合基因生物信息学方面的相关研究,以癌基因为切入点,从天然嵌合基因的融合特点、转录、调控,以及融合蛋白的结构域组合形式和功能等方面,结合本研究组前期的相关工作,综述了嵌合基因融合结构和功能的研究进展,探讨了当前研究工作的困难与挑战,并对嵌合规律在新基因设计的应用作了展望。

关键词: 嵌合基因, 嵌合RNA, 嵌合蛋白, 基因表达

Abstract:

The natural chimeric gene is a novel gene fused by two or more independent genes. Its discovery extends the classical gene concept that one gene resides in one chromosomal locus. During tumorigenesis, many chimeric genes lead to tumor-related diseases; therefore, it is one molecular marker for cancer diagnosis. In this review, we summarize the research progress of chimeric genes in terms of their feature, structure, transcription, regulation and function. We pinpoint the difficulties and challenges in the current research work, and discuss the application of chimeric gene in the new gene design.

Key words: chimeric gene, chimeric RNA, chimeric protein, gene expression

李迎侠, 张婷婷, 马磊. 天然嵌合基因的结构特性及其对基因设计的启示[J]. 遗传, 2018, 40(2): 135-144.

Li Yingxia, Zhang Tingting, Ma Lei. Structural characteristics of natural chimeric genes and their implications for gene design[J]. Hereditas(Beijing), 2018, 40(2): 135-144.

参考文献

[1]	Ma L , Zhang TT . Chimeric Gene: The Brige in Biological Molecular Network. Beijing: Jiuzhou Press, 2016.
[1]	马磊, 张婷婷 . 嵌合基因——生物分子网络的桥梁. 北京: 九州出版社, 2016. [DOI]
[2]	Mertens F , Antonescu CR , Mitelman F . Gene fusions in soft tissue tumors: recurrent and overlapping pathogenetic themes. Genes Chromosomes Cancer, 2016, 55( 4): 291- 310.
[3]	Leyten GH , Hessels D , Jannink SA , Smit FP, de Jong H, Cornel EB, de Reijke TM, Vergunst H, Kil P, Knipscheer BC, van Oort IM, Mulders PF, Hulsbergen-van de Kaa CA, Schalken JA. Prospective multicentre evaluation of PCA3 and TMPRSS2-ERG gene fusions as diagnostic and prognostic urinary biomarkers for prostate cancer. Eur Urol, 2014, 65( 3): 534- 542.
[4]	Minca EC , Portier BP , Wang Z , Lanigan C , Farver CF , Feng Y , Ma PC , Arrossi VA , Pennell NA , Tubbs RR . ALK status testing in non-small cell lung carcinoma: correlation between ultrasensitive IHC and FISH. J Mol Diagn, 2013, 15( 3): 341- 346.
[5]	Kumar-Sinha C , Kalyana-Sundaram S , Chinnaiyan AM . Landscape of gene fusions in epithelial cancers: seq and ye shall find. Genome Med, 2015, 7: 129.
[6]	Eck MJ , Manley PW . The interplay of structural information and functional studies in kinase drug design: insights from BCR-Abl. Curr Opin Cell Biol, 2009, 21( 2): 288- 295.
[7]	Gorohovski A , Tagore S , Palande V , Malka A , Raviv-Shay D , Frenkel-Morgenstern M . ChiTaRS-3.1-the enhanced chimeric transcripts and RNA-seq database matched with protein-protein interactions. Nucleic Acids Res, 2017, 45( D1): D790- D795.
[8]	Nowell PC , Hungerford DA . A minute chromosome in human chronic granulocytic leukaemia. Science, 1960, 132( 3438): 1497.
[9]	Fox Chase Cancer Center. 50th Anniversary of the Discovery of the Philadelphia Chromsome.[DOI]
[10]	Rowley JD . Letter: a new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining. Nature, 1973, 243( 5405): 290- 293.
[11]	Stephens PJ , McBride DJ, Lin ML, Varela I, Pleasance ED, Simpson JT, Stebbings LA, Leroy C, Edkins S, Mudie LJ, Greenman CD, Jia MM, Latimer C, Teague JW, Lau KW, Burton J, Quail MA, Swerdlow H, Churcher C, Natrajan R, Sieuwerts AM, Martens JW, Silver DP, Langer?d A, Russnes HEG, Foekens JA, Reis-Filho JS, van't Veer L, Richardson AL, B?rresen-Dale AL, Campbell PJ, Futreal PA, Stratton MR. Complex landscapes of somatic rearrangement in human breast cancer genomes. Nature, 2009, 462( 7276): 1005- 1010.
[12]	Muhle R , Trentacoste SV , Rapin I . The genetics of autism. Pediatrics, 2004, 113( 5): e472- e486.
[13]	Kim J , Kim S , Ko S , In YH , Moon HG , Ahn SK , Kim MK , Lee M , Hwang JH , Ju YS , Kim JI , Noh DY , Kim S , Park JH , Rhee H , Kim S , Han W . Recurrent fusion transcripts detected by whole-transcriptome sequencing of 120 primary breast cancer samples. Genes Chromosomes Cancer, 2015, 54( 11): 681- 691.
[14]	Robinson DR , Kalyana-Sundaram S , Wu YM , Shankar S , Cao XH , Ateeq B , Asangani IA , Iyer M , Maher CA , Grasso CS , Lonigro RJ , Quist M , Siddiqui J , Mehra R , Jing XJ , Giordano TJ , Sabel MS , Kleer CG , Palanisamy N , Natrajan R , Lambros MB , Reis-Filho JS , Kumar-Sinha C , Chinnaiyan AM . Functionally recurrent rearrangements of the MAST kinase and Notch gene families in breast cancer. Nat Med, 2011, 17( 12): 1646- 1651.
[15]	Edgren H , Murumagi A , Kangaspeska S , Nicorici D , Hongisto V , Kleivi K , Rye IH , Nyberg S , Wolf M , Borresen-Dale AL , Kallioniemi O . Identification of fusion genes in breast cancer by paired-end RNA-sequencing. Genome Biol, 2011, 12( 1): R6.
[16]	Seo JS , Ju YS , Lee WC , Shin JY , Lee JK , Bleazard T , Lee J , Jung YJ , Kim JO , Shin JY , Yu SB , Kim J , Lee ER , Kang CH , Park IK , Rhee H , Lee SH , Kim JI , Kang JH , Kim YT . The transcriptional landscape and mutational profile of lung adenocarcinoma. Genome Res, 2012, 22( 11): 2109- 2119.
[17]	Teles Alves I , Hartjes T , McClellan E, Hiltemann S, B?ttcher R, Dits N, Temanni MR, Janssen B, van Workum W, van der Spek P, Stubbs A, de Klein A, Eussen B, Trapman J, Jenster G. Next-generation sequencing reveals novel rare fusion events with functional implication in prostate cancer. Oncogene, 2015, 34( 5): 568- 577.
[18]	Pflueger D , Terry S , Sboner A , Habegger L , Esgueva R , Lin PC , Svensson MA , Kitabayashi N , Moss BJ , MacDonald TY, Cao XH, Barrette T, Tewari AK, Chee MS, Chinnaiyan AM, Rickman DS, Demichelis F, Gerstein MB, Rubin MA. Discovery of non-ETS gene fusions in human prostate cancer using next-generation RNA sequencing. Genome Res, 2011, 21( 1): 56- 67.
[19]	Kannan K , Wang L , Wang J , Ittmann MM , Li W , Yen L . Recurrent chimeric RNAs enriched in human prostate cancer identified by deep sequencing. Proc Natl Acad Sci USA, 2011, 108( 22): 9172- 9177.
[20]	Luo JH , Liu S , Zuo ZH , Chen R , Tseng GC , Yu YP . Discovery and classification of fusion transcripts in prostate cancer and normal prostate tissue. Am J Pathol, 2015, 185( 7): 1834- 1845.
[21]	Kim HP , Cho GA , Han SW , Shin JY , Jeong EG , Song SH , Lee WC , Lee KH , Bang D , Seo JS , Kim JI , Kim TY . Novel fusion transcripts in human gastric cancer revealed by transcriptome analysis. Oncogene, 2014, 33( 47): 5434- 5441.
[22]	Steidl C , Shah SP , Woolcock BW , Rui LX , Kawahara M , Farinha P , Johnson NA , Zhao YJ , Telenius A , Neriah SB , McPherson A, Meissner B, Okoye UC, Diepstra A, van den Berg A, Sun M, Leung G, Jones SJ, Connors JM, Huntsman DG, Savage KJ, Rimsza LM, Horsman DE, Staudt LM, Steidl U, Marra MA, Gascoyne RD. MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers. Nature, 2011, 471( 7338): 377- 381.
[23]	Pane F , Intrieri M , Quintarelli C , Izzo B , Muccioli GC , Salvatore F . BCR/ABL genes and leukemic phenotype: from molecular mechanisms to clinical correlations. Oncogene, 2002, 21( 56): 8652- 8667.
[24]	Tomlins SA , Laxman B , Dhanasekaran SM , Helgeson BE , Cao XH , Morris DS , Menon A , Jing XJ , Cao Q , Han B , Yu JD , Wang L , Montie JE , Rubin MA , Pienta KJ , Roulston D , Shah RB , Varambally S , Mehra R , Chinnaiyan AM . Distinct classes of chromosomal rearrangements create oncogenic ETS gene fusions in prostate cancer. Nature, 2007, 448( 7153): 595- 599.
[25]	Milhausen M , Nelson RG , Sather S , Selkirk M , Agabian N . Identification of a small RNA containing the trypanosome spliced leader: a donor of shared 5° sequences of trypanosomatid mRNAs? Cell, 1984, 38( 3): 721- 729.
[26]	Koller B , Fromm H , Galun E , Edelman M . Evidence for in vivo trans splicing of pre-mRNAs in tobacco chloroplasts. Cell, 1987, 48( 1): 111- 119.
[27]	Kück U , Choquet Y , Schneider M , Dron M , Bennoun P . Structural and transcription analysis of two homologous genes for the P700 chlorophyll a-apoproteins in Chlamydomonas reinhardii: evidence for in vivo trans-splicing. EMBO J, 1987, 6( 8): 2185- 2195.
[28]	Dandekar T , Sibbald PR . Trans-splicing of pre-mRNA is predicted to occur in a wide range of organisms including vertebrates. Nucleic Acids Res, 1990, 18( 16): 4719- 4725.
[29]	Caudevilla C , Serra D , Miliar A , Codony C , Asins G , Bach M , Hegardt FG . Natural trans-splicing in carnitine octanoyltransferase pre-mRNAs in rat liver. Proc Natl Acad Sci USA, 1998, 95( 21): 12185- 12190.
[30]	Frantz SA , Thiara AS , Lodwick D , Ng LL , Eperon IC , Samani NJ . Exon repetition in mRNA. Proc Natl Acad Sci USA, 1999, 96( 10): 5400- 5405.
[31]	Rigatti R , Jia JH , Samani NJ , Eperon IC . Exon repetition: a major pathway for processing mRNA of some genes is allele-specific. Nucleic Acids Res, 2004, 32( 2): 441- 446.
[32]	Honeyman JN , Simon EP , Robine N , Chiaroni-Clarke R , Darcy DG , Lim IIP , Gleason CE , Murphy J , Rosenberg BR , Teegan L , Takacs CN , Botero S , Belote R , Germer S , Emde AK , Vacic V , Bhanot U , LaQuaglia MP, Simon SM. Detection of a recurrent DNAJB1-PRKACA chimeric transcript in fibrolamellar hepatocellular carcinoma. Science, 2014, 343( 6174): 1010- 1014.
[33]	Ma L , Yang SL , Zhao WM , Tang ZL , Zhang TT , Li K . Identification and analysis of pig chimeric mRNAs using RNA sequencing data. BMC Genomics, 2012, 13: 429.
[34]	Roukos V , Misteli T . The biogenesis of chromosome translocations. Nat Cell Biol, 2014, 16( 4): 293- 300.
[35]	Ortiz de Mendíbil I , Vizmanos JL , Novo FJ . Signatures of selection in fusion transcripts resulting from chromosomal translocations in human cancer. PLoS One, 2009, 4( 3): e4805.
[36]	Yoshihara K , Wang Q , Torres-Garcia W , Zheng S , Vegesna R , Kim H , Verhaak RGW . The landscape and therapeutic relevance of cancer-associated transcript fusions. Oncogene, 2015, 34( 37): 4845- 4854.
[37]	Frenkel-Morgenstern M , Valencia A . Novel domain combinations in proteins encoded by chimeric transcripts. Bioinformatics, 2012, 28( 12): i67- i74.
[38]	Lam K , Zhang DE . RUNX1 and RUNX1-ETO: roles in hematopoiesis and leukemogenesis. Front Biosci (Landmark Ed), 2012, 17: 1120- 1139.
[39]	Peterson LF , Zhang DE . The 8;21 translocation in leukemogenesis. Oncogene, 2004, 23( 24): 4255- 4262.
[40]	Ptasinska A , Assi SA , Mannari D , James SR , Williamson D , Dunne J , Hoogenkamp M , Wu M , Care M , McNeill H, Cauchy P, Cullen M, Tooze RM, Tenen DG, Young BD, Cockerill PN, Westhead DR, Heidenreich O, Bonifer C. Depletion of RUNX1/ETO in t(8;21) AML cells leads to genome-wide changes in chromatin structure and transcription factor binding. Leukemia, 2012, 26( 8): 1829- 1841.
[41]	Kim P , Yoon S , Kim N , Lee S , Ko M , Lee H , Kang H , Kim J , Lee S . ChimerDB 2.0—a knowledgebase for fusion genes updated. Nucleic Acids Res, 2010, 38( Suppl. 1): D81- D85.
[42]	Li X , Zhao L , Jiang HF , Wang W . Short homologous sequences are strongly associated with the generation of chimeric RNAs in eukaryotes. J Mol Evol, 2009, 68( 1): 56- 65.
[43]	Frenkel-Morgenstern M , Lacroix V , Ezkurdia I , Levin Y , Gabashvili A , Prilusky J, del Pozo A, Tress M, Johnson R, Guigo R, Valencia A. Chimeras taking shape: potential functions of proteins encoded by chimeric RNA transcripts. Genome Res, 2012, 22( 7): 1231- 1242.
[44]	Zayed A , Couban S , Hayne O , Sparavalo N , Shawwa A , Sadek I , Greer W . Acute promyelocytic leukemia: a novel PML/RARα fusion that generates a frameshift in the RARα transcript and ATRA resistance. Leuk Lymphoma, 2006, 48( 3): 489- 496.
[45]	Shugay M , Ortiz de Mendíbil I, Vizmanos JL, Novo FJ. Genomic hallmarks of genes involved in chromosomal translocations in hematological cancer. PLoS Comput Biol, 2012, 8( 12): e1002797.
[46]	Pawson T , Warner N . Oncogenic re-wiring of cellular signaling pathways. Oncogene, 2007, 26( 9): 1268- 1275.
[47]	Prensner JR , Chinnaiyan AM . The emergence of lncRNAs in cancer biology. Cancer Discov, 2011, 1( 5): 391- 407.
[48]	Nakamura Y , Takahashi N , Kakegawa E , Yoshida K , Ito Y , Kayano H , Niitsu N , Jinnai I , Bessho M . The GAS5 (growth arrest-specific transcript 5) gene fuses to BCL6 as a result of t(1;3)(q25;q27) in a patient with B-cell lymphoma. Cancer Genet Cytogenet, 2008, 182( 2): 144- 149.
[49]	Shahrouki P , Larsson E . The non-coding oncogene: a case of missing DNA evidence? Front Genet, 2012, 3: 170.
[50]	Ren SC , Peng ZY , Mao JH , Yu YW , Yin CJ , Gao X , Cui ZL , Zhang JB , Yi K , Xu WD , Chen C , Wang FB , Guo XW , Lu J , Yang J , Wei M , Tian ZJ , Guan YH , Tang L , Xu CL , Wang LH , Gao X , Tian W , Wang J , Yang HM , Wang J , Sun YH . RNA-seq analysis of prostate cancer in the Chinese population identifies recurrent gene fusions, cancer-associated long noncoding RNAs and aberrant alternative splicings. Cell Res, 2012, 22( 5): 806- 821.
[51]	Zhu YS , Ren SC , Jing TL , Cai XB , Liu YW , Wang FB , Zhang W , Shi XL , Chen R , Shen J , Lu J , Xu CL , Wang HQ , Wang HF , Wang Y , Liu B , Li YM , Fang ZY , Guo F , Qiao M , Shen D , Lu X , Gao X , Hou JG , Sun YH . Clinical utility of a novel urine-based gene fusion TTTY15-USP9Y in predicting prostate biopsy outcome. Urol Oncol: Semin Orig Investig, 2015, 33(9): 384.e9-384.e20.
[52]	Shi J , Li YM , Fang XD . The mechanism and clinical significance of long noncoding RNA-mediated gene expression via nuclear architecture. Hereditas (Beijing), 2017, 39( 3): 189- 199.
[52]	施剑, 李艳明, 方向东 . 长链非编码RNA通过细胞核高级结构调控真核基因表达及其临床意义. 遗传, 2017, 39( 3): 189- 199.
[53]	Lai J , An JY , Seim I , Walpole C , Hoffman A , Moya L , Srinivasan S , Perry-Keene JL , Australian Prostate Cancer Bioresource, Wang CW, Lehman ML, Nelson CC, Clements JA, Batra J. Fusion transcript loci share many genomic features with non-fusion loci. BMC Genomics, 2015, 16: 1021.
[54]	Qin FJ , Song ZG , Babiceanu M , Song YS , Facemire L , Singh R , Adli M , Li H . Discovery of CTCF-sensitive cis- spliced fusion RNAs between adjacent genes in human prostate cells. PLoS Genet, 2015, 11( 2): e1005001.
[55]	Gingeras TR . Implications of chimaeric non-co-linear transcripts. Nature, 2009, 461( 7261): 206- 211.
[56]	Houseley J , Tollervey D . Apparent non-canonical trans- splicing is generated by reverse transcriptase in vitro. PLoS One, 2010, 5( 8): e12271.
[57]	Yu CY , Liu HJ , Hung LY , Kuo HC , Chuang TJ . Is an observed non-co-linear RNA product spliced in trans, in cis or just in vitro? Nucleic Acids Res, 2014, 42( 14): 9410- 9423.
[58]	Akiva P , Toporik A , Edelheit S , Peretz Y , Diber A , Shemesh R , Novik A , Sorek R . Transcription-mediated gene fusion in the human genome. Genome Res, 2006, 16( 1): 30- 36.
[59]	Parra G , Reymond A , Dabbouseh N , Dermitzakis ET , Castelo R , Thomson TM , Antonarakis SE , Guigó R . Tandem chimerism as a means to increase protein complexity in the human genome. Genome Res, 2006, 16( 1): 37- 44.
[60]	Denoeud F , Kapranov P , Ucla C , Frankish A , Castelo R , Drenkow J , Lagarde J , Alioto T , Manzano C , Chrast J , Dike S , Wyss C , Henrichsen CN , Holroyd N , Dickson MC , Taylor R , Hance Z , Foissac S , Myers RM , Rogers J , Hubbard T , Harrow J , Guigó R , Gingeras TR , Antonarakis SE , Reymond A . Prominent use of distal 5' transcription start sites and discovery of a large number of additional exons in ENCODE regions. Genome Res, 2007, 17( 6): 746- 759.
[61]	Djebali S , Lagarde J , Kapranov P , Lacroix V , Borel C , Mudge JM , Howald C , Foissac S , Ucla C , Chrast J , Ribeca P , Martin D , Murray RR , Yang X , Ghamsari L , Lin C , Bell I , Dumais E , Drenkow J , Tress ML , Gelpí JL , Orozco M , Valencia A, van Berkum NL, Lajoie BR, Vidal M, Stamatoyannopoulos J, Batut P, Dobin A, Harrow J, Hubbard T, Dekker J, Frankish A, Salehi-Ashtiani K, Reymond A, Antonarakis SE, Guigó R, Gingeras TR. Evidence for transcript networks composed of chimeric RNAs in human cells. PLoS One, 2012, 7( 1): e28213.
[62]	Casado-Vela J , Lacal JC , Elortza F . Protein chimerism: novel source of protein diversity in humans adds complexity to bottom-up proteomics. Proteomics, 2013, 13( 1): 5- 11.
[63]	Li H , Wang JL , Ma XY , Sklar J . Gene fusions and RNA trans-splicing in normal and neoplastic human cells. Cell Cycle, 2009, 8( 2): 218- 222.
[64]	Rickman DS , Pflueger D , Moss B , VanDoren VE, Chen CX, de la Taille AK, Kuefer R, Tewari AK, Setlur SR, Demichelis F, Rubin MA. SLC45A3-ELK4 is a novel and frequent erythroblast transformation-specific fusion transcript in prostate cancer. Cancer Res, 2009, 69( 7): 2734- 2738.
[65]	Nome T , Hoff AM , Bakken AC , Rognum TO , Nesbakken A , Skotheim RI . High frequency of fusion transcripts involving TCF7L2 in colorectal cancer: novel fusion partner and splice variants. PLoS One, 2014, 9( 3): e91264.
[66]	Long MY , Langley CH . Natural selection and the origin of Jingwei, a chimeric processed functional gene in Drosophila. Science, 1993, 260( 5104): 91- 95.
[67]	Lynch M , Conery JS . The evolutionary fate and consequences of duplicate genes. Science, 2000, 290( 5494): 1151- 1155.

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

天然嵌合基因的结构特性及其对基因设计的启示

Structural characteristics of natural chimeric genes and their implications for gene design

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	禹奇超,宋彬,邹轩轩,王岭,刘德权,李波,马昆. 乳腺癌癌旁组织特异性表达基因分析[J]. 遗传, 2019, 41(7): 625-633.
[2]	石田培,张莉. 全转录组学在畜牧业中的应用[J]. 遗传, 2019, 41(3): 193-205.
[3]	宁椿游,何梦楠,唐茜子,朱庆,李明洲,李地艳. 基于Hi-C技术哺乳动物三维基因组研究进展[J]. 遗传, 2019, 41(3): 215-233.
[4]	丁庆倩,王小婷,胡利琴,齐欣,葛林豪,徐伟亚,徐兆师,周永斌,贾冠清,刁现民,闵东红,马有志,陈明. 谷子MYB类转录因子SiMYB42提高转基因拟南芥低氮胁迫耐性[J]. 遗传, 2018, 40(4): 327-338.
[5]	禚建树, 荆晓燕, 杜欣, 杨秀芹. 哺乳动物嵌合RNAs产生机制：cis-SAGe[J]. 遗传, 2018, 40(2): 145-154.
[6]	马磊, 张婷婷. 应用嵌合基因实例拓展遗传学染色体畸变的教学[J]. 遗传, 2018, 40(12): 1129-1135.
[7]	徐宗昌,孔英珍. 普通烟草CESA基因家族成员的鉴定、亚细胞定位及表达分析[J]. 遗传, 2017, 39(6): 512-524.
[8]	施剑,李艳明,方向东. 长链非编码RNA通过细胞核高级结构调控真核基因表达及其临床意义[J]. 遗传, 2017, 39(3): 189-199.
[9]	魏凯,马磊. 高通量测序时代下持家基因定义的发展[J]. 遗传, 2017, 39(2): 127-134.
[10]	许璟瑾, 张文娟, 王静怡, 姚丽云, 潘裕添, 欧一新, 薛钰, . 金线莲抑制斑马鱼黑色素形成的活性组分筛选及机理研究[J]. 遗传, 2017, 39(12): 1178-1187.
[11]	路畅, 黄银花. 动物长链非编码RNA研究进展[J]. 遗传, 2017, 39(11): 1054-1065.
[12]	崔婷婷, 邢天宇, 褚衍凯, 李辉, 王宁. PPARγ在脂肪生成中的遗传和表观遗传调控[J]. 遗传, 2017, 39(11): 1066-1077.
[13]	向小华, 吴新儒, 晁江涛, 杨明磊, 杨帆, 陈果, 刘贯山, 王元英. 普通烟草WRKY基因家族的鉴定及表达分析[J]. 遗传, 2016, 38(9): 840-856.
[14]	翟亚男, 许泉, 郭亚, 吴强. 原钙粘蛋白基因簇调控区域中成簇的CTCF结合位点分析[J]. 遗传, 2016, 38(4): 323-336.
[15]	刘娟, 孙艳, 姜强, 杨春红, 黄金明, 李建斌, 侯明海, 仲跻峰, 王长法, 刘保申. INCENP基因功能性单倍型可调控启动子活性并影响公牛精液品质[J]. 遗传, 2016, 38(1): 62-71.