ANAC092参与调控花药发育的功能初探

doi:10.3724/SP.J.1005.2013.00913

遗传 ›› 2013, Vol. 35 ›› Issue (7): 913-922.doi: 10.3724/SP.J.1005.2013.00913

ANAC092参与调控花药发育的功能初探

李捷^1,2,3, 陈旭^2,3, 罗莉琼^{2, 3}, 于晶¹, 明凤^2,3

1. 上海师范大学生命与环境科学学院, 上海 200234 2. 复旦大学生命科学学院植物科学研究所, 上海 200433 3. 复旦大学遗传工程国家重点实验室, 上海 200433

收稿日期:2013-03-27 修回日期:2013-05-02 出版日期:2013-07-20 发布日期:2013-07-25
通讯作者: 于晶 E-mail:yujing@shnu.edu.cn
基金资助:
转基因生物新品种培育科技重大专项(编号：2011ZX08009-001)和上海市自然科学基金项目(编号：12ZR1402300)资助

Functions of ANAC092 involved in regulation of anther development in Arabidopsis thaliana

LI Jie^1,2,3, CHEN Xu^2,3, LUO Li-Qiong^2,3, YU Jing¹, MING Feng^2,3

1. College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, China 2. Institute of Plant Biology, School of Life Science, Fudan University, Shanghai 200433, China 3. State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China

Received:2013-03-27 Revised:2013-05-02 Online:2013-07-20 Published:2013-07-25

摘要/Abstract

摘要： NAC家族转录因子是高等植物特有的一类转录因子, 功能广泛, 这类蛋白在植物次生生长、细胞分裂、植物衰老、尤其在激素和信号途径起关键调控作用。ANAC092已报道参与侧根发育, 并与衰老相关。为研究ANAC092基因在花药发育过程中的功能, 文章构建了拟南芥ANAC092启动子的GUS载体, 结合原位杂交分析结果表明, ANAC092在花药发育过程中时序性表达, 在花药发育的8~11期绒毡层表达, 其中在9~10期的表达量达到最高值, 与AMS(Aborted microspores)的表达时期有重合。构建ANAC092过表达体系, 筛选出转基因纯合株系。与野生型相比, 过表达ANAC092转基因植株中花粉数量减少, 花粉粒的长度增加。qRT-PCR结果表明, 过表达株系中与花粉发育相关的基因SPL、EMS1、DYT1、AMS的表达量上调。结合生物信息学分析表明, ANAC092启动子序列中有7个AMS的结合位点, 因此推测ANAC092可能位于AMS的下游而参与花药发育过程。

关键词: 花药发育, 拟南芥, ANAC092, AMS

Abstract: NAC family is a class of transcription factors that have been typically found in plant with various functions. This type of genes plays a key regulatory role in secondary plant growth, cell division, senescence, especially in the hormone and signaling pathways. ANAC092 was reported to function in relation to lateral root development and senescence. The promoter and in situ hybridization analyses showed that ANAC092 was expressed temporally in the process of anther development. The gene was expressed in tapetum at stages 8–11 of anther development and reached the highest amount at stages 9–10, which was overlapped with the expression period of AMS (Aborted microspores). In this study, we constructed ANAC092 over-expression system, and identified homozygous transgenic lines. Compared with the wild type, the number of pollen grains in the transgenic line was decreased significantly, but the length of pollen grain was increased. qRT-PCR analysis showed that the expression of genes related to pollen development, e.g., SPL, EMS1, DYT1, and AMS was in-creased in the over-expression plants. Bioinformatics results showed that ANAC092 promoter sequence possessed seven AMS binding sites. All the results showed that ANAC092 is possibly located in the downstream of AMS and plays an im-portant role in the process of pollen development.

Key words: anther development, Arabidopsis, ANAC092, AMS

李捷陈旭罗莉琼于晶明凤. ANAC092参与调控花药发育的功能初探[J]. 遗传, 2013, 35(7): 913-922.

LI Jie CHEN Xu LUO Li-Qiong YU Jing MING Feng. Functions of ANAC092 involved in regulation of anther development in Arabidopsis thaliana[J]. HEREDITAS, 2013, 35(7): 913-922.

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ANAC092参与调控花药发育的功能初探

Functions of ANAC092 involved in regulation of anther development in Arabidopsis thaliana

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