遗传 ›› 1991, Vol. 18 ›› Issue (3): 235-238.

• 论文 • 上一篇    

玉米花粉单倍体植株染色体上异染色质的变异

谷明光;林 侠   

  1. 中国科学院遗传研究所,北京100101
  • 收稿日期:1900-01-01 修回日期:1900-01-01

Variation of Heterochromatin on the Chromosomes of the Maize Pollen Haploid Plan ts

Gu Mingguang, Lin Xia   

  1. Lnstitute of Gentics,Academia Sinica,Beijing100101
  • Received:1900-01-01 Revised:1900-01-01

摘要: 我们用Giemsa BSG C-带技术检查了玉米花药培养获得的花粉单倍体植株根尖细胞染色体上 异染色质的变异,观察结果表明,有的植株所显示的C-带数目是与供体植株的相一致,有的 植株所显示的C-带数目则发生了显著变化,其中有的增加,有的减少。并讨论了异染色质发 生变异的可能原因。还相应地观察到间期核中染色中心的变化是与中期染色体上C-带数目的 变化相一致。

关键词: 异染色质带, 变异, 玉米花粉单倍体植株, 染色体, 玉米花粉单倍体植株, 染色体, 异染色质带, 变异

Abstract: In this paper we studied changes of heterochromatin on the chromosomes of maize pollen haploid plants with Giemsa BSG C-banding technique.The main results abtai ned were as follows: The Giemsa C-banding of some haploid plants showed to be the same as that of donor.They had prominent heterochromatic bands on five of the ten chromosomes,w hich is half of the banding number of donor plants.Because the pollen haploid pl ants possessed only one set of chromosomes,the donor diploid plants possessed tw o sets of chromosomes.The other haploid plants had 3,4,6 and 7 bands,respectivel y.It is,therefore,considred that in invitro culture there are not only visible c hanges of chromosome number and structure,but also changes of chromosome heteroc hromatin.It showed that the number of heterochromatic chromacentres in nuclei at interphase stage was close to that of number of banding on chromosomes at metap hase stage.

Key words: Variation, Maize pollen haploid plant, Chromosome, Geterochromatin banding