遗传 ›› 2004, Vol. 26 ›› Issue (3): 0-308.

• 论文 •    下一篇

鸡杂种与纯种间CIDH基因的差异表达 及其与杂种优势的关系

王 栋; 张 沅; 孙东晓; 俞 英; 徐桂云; 李俊英WANG Dong1;2; ZHANG Yuan1; SUN Dong-Xiao1; YU Ying1; XU Gui-Yun1; LI Jun-Ying1   

  1. 1.中国农业大学动物科技学院,北京,100094 College of Animal Science and Technology, China Agricultural University, Beijing 100094 China
  • 收稿日期:1900-01-01 出版日期:2004-06-10 发布日期:2004-06-10

Differential Expression of CIDH Gene Between Purebreds and Hybrids in Chicken and Relationship with Heterosis

  • Received:1900-01-01 Published:2004-06-10 Online:2004-06-10

摘要:

本研究运用银染mRNA差异显示技术进行鸡杂种优势分子遗传机理研究。在检测白洛克肉鸡(EE)和 丝毛乌骨鸡(CC)纯种及其正反交杂种间的mRNA差异表达情况时,发现一个只在杂种表达的475bp的cDNA片段:CE15A15。经过反向Northern印记杂交和RT-PCR验证,发现该片段在杂种中表达增强。序列比对结果表明该片段与鸡部分cDNA序列(ID :AW198493)的相似程度高达97%, 而这一序列又相似于墨西哥野鼠的细胞质异柠檬酸脱氢酶(CIDH)基因(ID:AF048831),说明CE15A15为鸡CIDH基因的部分cDNA。表型数据的统计分析表明,丝毛乌骨鸡(CC)和白洛克肉鸡(EE)正反交杂种(CE、EC)的活重和屠体性状与同期纯种相比都具有负优势,而脂肪性状则具有不同程度的正优势。由于CIDH直接参与了动物机体脂肪酸合成代谢,由此推断,该cDNA片段在鸡纯种和杂种中的差异表达可能与杂种优势形成有关。

Abstract : The technique of silver staining mRNA Differential Display was used to check the molecular genetic mechanism of heterosis in chicken. A differentially expressed fragment CE15A15, which was only expressed in two crossbreds, was found between purebreds and crossbreds. It is proved that the fragment intensively expresses in crossbreds through reverse Northern dot blot and RT-PCR. It shares 97% of sequence identity with a partial cDNA sequence of chicken that has been deposited in GenBank (AW198493) and believed to be identity with the cytosolic isocitrate dehydrogenase (CIDH) gene(ID:AF048831)of Microtus mexicanus. This suggests that the identified fragment may be a partial cDNA sequence of CIDH gene. The statistic analyses of phenotypes showed that the body weight and the carcass traits in crossbred of Silk Chicken (CC)×White Polymouth Rock (EE) and White Polymouth Rock (EE)×Silk Chicken (CC) had negative heterosis, while their fatty traits had positive heterosis. CIDH directly participates in the biosynthesis of fatty acids. This indicates that differential expression of the fragment between purebreds and crossbreds might be closely related to heterosis of crossbreds.

关键词: chicken, CIDH, 杂种优势
Key words,
CIDH,