遗传 ›› 2002, Vol. 24 ›› Issue (2): 146-148.

• 论文 • 上一篇    下一篇

猪H-FABP基因多态片段的序列分析

曹红鹤;张桂香;王立贤;李宏滨;郑友民 CAO Hong-he;ZHANG Gui-xiang;WANG Li-xian;LI Hong-bin;ZHENG You-min   

  1. 中国农业科学院畜牧研究所,北京 100094 Institute of Animal Science,Chinese Academy of Agricultural Sciences,100094 Beijing,China
  • 收稿日期:1900-01-01 出版日期:2002-04-10 发布日期:2002-04-10

Sequence Analysis of Polymorphic Fragments of Porcine H-FABP Gene

  • Received:1900-01-01 Online:2002-04-10 Published:2002-04-10

摘要: 利用PCR-RFLP方法在猪H-FABP基因内确定了3个变异酶切位点,分别为5′-上游区HinfI-RFLP、内含子2的HaeIII-RFLP和HinfI*-RFLP(HinfI和HinfI*代表不同区域的HinfI酶切反应)。对每个多态片段进行克隆测序分析,结果表明,5′-上游区HinfI-RFLP是由于1324位的碱基T→C的突变引起;在内含子2中,HaeIII-RFLP变异酶切位点在1811位,发生了C到G的突变;HinfI*-RFLP是由于1970位发生T→C的碱基替换。
Abstract:Three variant restriction sites of porcine H-FABP gene,including HinfI-RFLP in 5′-upsream,HaeIII-RFLP and HinfI*-RFLP in intron 2,were confirmed by PCR-RFLP method.The polymorphic fragments were cloned and sequenced.The results revealed a single nucleotide substitution of T→C at position 1324 for HinfI-RFLP,C→G at position 1811 for HaeIII-RFLP and T→C at position 1970 for HinfI*-RFLP,respectively.

关键词: 猪, H-FABP基因, H-FABP gene, pig, 序列分析
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