CRISPR/Cas9基因组编辑技术在农业动物中的应用

doi:10.16288/j.yczz.15-398

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HEREDITAS(Beijing) ›› 2016, Vol. 38 ›› Issue (3): 217-226.doi: 10.16288/j.yczz.15-398

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Application of CRISPR/Cas9 mediated genome editing in farm animals

Yuyun Xing, Qiang Yang, Jun Ren

State Key Laboratory for Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University, Nanchang 330045, China

Received:2015-09-21 Revised:2016-01-03 Online:2016-03-20 Published:2016-03-20
Supported by:
Supported by the National Science and Technology Major Project of China (No.2014ZX08006-005) and the National Natural Science Foundation of China (No.31560304)

Abstract

Abstract: CRISPR (Clustered regularly interspaced short palindromic repeats)/Cas (CRISPR associated proteins) is an acquired immune system found in bacteria and archaea that fight against invasion of viruses or plasmids. CRISPR/Cas systems are currently classified into three main types: I, II and III, of which type II has relatively simple components. The CRISPR/Cas9 technology modified from type II CRISPR/Cas system has been developed as an efficient genome editing tool. Since the initial application of the CRISPR/Cas9 technology in mammals in 2013, the reports of this system for genomic editing has skyrocketed. Farm animals are not only economically important animals, but also ideal animal models for human diseases and biomedical studies. In this review, we summarize the applications of CRISPR/Cas9 in farm animals, briefly describe the off-target effects and the main solutions, and finally highlight the future perspectives of this technology.

Key words: CRISPR/Cas9, genome editing, farm animals, off-target

Yuyun Xing, Qiang Yang, Jun Ren. Application of CRISPR/Cas9 mediated genome editing in farm animals[J]. HEREDITAS(Beijing), 2016, 38(3): 217-226.

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Application of CRISPR/Cas9 mediated genome editing in farm animals

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