INCENP基因功能性单倍型可调控启动子活性并影响公牛精液品质

doi:10.16288/j.yczz.15-230

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HEREDITAS(Beijing) ›› 2016, Vol. 38 ›› Issue (1): 62-71.doi: 10.16288/j.yczz.15-230

• Research Articles • Previous Articles Next Articles

Functional haplotypes of INCENP affect promoter activity and bovine semen quality

Juan Liu^{1, 2}, Yan Sun², Qiang Jiang², Chunhong Yang², Jinming Huang², Jianbin Li², Minghai Hou², Jifeng Zhong², Changfa Wang², Baoshen Liu¹

1. College of Agronomic Sciences in Shandong Agricultural University, Taian 271000, China;
2. Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan 250131, China

Received:2015-05-26 Revised:2015-09-08 Online:2016-01-20 Published:2016-01-20
Supported by:
[Supported by the National Natural Science Foundation of China (No; 31401049, 31401050),the Well-Bred Program of Shandong Province (No; 2014LZ), the Program of National Cow Industrial Technology System (No; CARS-37), and Bovine Innovation team of Shandong Province Agricultural Technology System (No; SDAIT-12-011-02)]

Abstract

Abstract: To explore the association between single nucleotide polymorphisms (SNPs) in the promoter region of the inner centromere protein (INCENP) gene and bovine semen quality, the haplotypes in 250 Chinese Holstein bulls were detected using PCR-RFLP method in this study. Two SNPs (g.-556 G>T, rs 136823901 and g.-692 C>T, rs 211010999) and three haplotypes (CG, TT, TG) were identified in the promoter region of INCENP. The genotype frequency and allele frequency of these two SNPs as well as the correlation between different SNP haplotype combinations and bovine semen quality were then analyzed. Our results showed that fresh sperm motility of the GT genotype was significantly higher than that of the GG genotype (P<0.05) at the SNP site g.-556 G>T, while fresh and frozen-thawed sperm motilities of the haplotype combinations H1H1(CCGG), H1H3(CTGT), H2H3(TTGT) and H3H3(TTTT) were significantly higher than that of H1H2 (P<0.05). To further study the possible mechanisms by which g.-556 G>T and g.-692 C>T affect semen quality, three haplotype plasmids were respectively transfected into MLTC-1 cells. The TG haplotype demonstrated the highest luciferase activity, suggesting that g.-556 G>T and g.-692 C>T are functional mutations which could regulate INCENP gene expression by affecting promoter activity and thus affect semen quality.

Key words: INCENP, SNP, haplotype, promoter region, semen quality, gene expression

Juan Liu, Yan Sun, Qiang Jiang, Chunhong Yang, Jinming Huang, Jianbin Li, Minghai Hou, Jifeng Zhong, Changfa Wang, Baoshen Liu. Functional haplotypes of INCENP affect promoter activity and bovine semen quality[J]. HEREDITAS(Beijing), 2016, 38(1): 62-71.

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Functional haplotypes of INCENP affect promoter activity and bovine semen quality

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