高等哺乳动物LEM结构域蛋白家族的研究进展

doi:10.16288/j.yczz.14-351

遗传 ›› 2015, Vol. 37 ›› Issue (2): 128-139.doi: 10.16288/j.yczz.14-351

高等哺乳动物LEM结构域蛋白家族的研究进展

胡清霞,高昂,曾炜佳,王妍馨,董金堂,朱正茂

南开大学生命科学学院,天津市蛋白质科学重点实验室,天津 300071

收稿日期:2014-10-14 修回日期:2015-01-05 出版日期:2015-02-20 发布日期:2015-01-19
通讯作者: 朱正茂,博士,副教授,研究方向：肿瘤发生和发展的分子机制研究,核膜组装与细胞周期调控。E-mail: zhuzhengmao@nankai.edu.cn E-mail:zhuzhengmao@nankai.edu.cn
作者简介:胡清霞,在读硕士,专业方向：细胞生物学。E-mail: 1150724550@qq.com
基金资助:
国家自然科学基金面上项目(编号：81470118)和南开大学教学改革项目“遗传学本科教学激励机制的研究与实践”

Structure and biological functions of mammalian LEM-Domain proteins

Qingxia Hu,Ang Gao,Weijia Zeng,Yanxin Wang,Jintang Dong,Zhengmao Zhu

Tianjin Key Laboratory of Protein Sciences, Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China

Received:2014-10-14 Revised:2015-01-05 Online:2015-02-20 Published:2015-01-19

摘要/Abstract

摘要： 在高等动物细胞开放式有丝分裂过程中,细胞核膜会发生高度有序的周期性去组装和装配的动态变化。近年的研究结果表明是LEM家族蛋白成员通过与BAF因子相互作用介导了内核膜、核纤层蛋白以及染色体之间的相互作用。LEM蛋白、核纤层蛋白以及BAF因子直接相互作用形成的三元复合体在结构与功能上是相互依赖的,在此结构与功能上组成的网络体系是形成细胞核的一些基本生物学过程的重要条件。该复合体在调控有丝分裂M期后期和末期染色体的正常分离、有丝分裂后核膜的重组装,细胞分裂间期细胞核及核膜形态维持,调控DNA复制和DNA损伤修复,调节基因表达和信号通路以及逆转录病毒感染等方面发挥着重要的生物学功能。并且LEM蛋白相关基因的异常对核纤层疾病和肿瘤的发生发展具有重要的影响。文章主要针对LEM蛋白家族成员的结构以及功能研究进展进行了详细的综述。

关键词: LEM蛋白, 核纤层蛋白, BAF, 有丝分裂, 核膜组装, 核膜核纤层病, 肿瘤发生

Abstract: During the process of open mitosis in higher eukaryotic cells, the nuclear envelope (NE) is disassembled and reassembled with highly organized and periodical dynamic morphological changes. Recent studies demonstrated that LEM-domain protein family mediates interactions among inner nuclear membrane, nuclear lamina protein and chromatin by interacting with barrier-to-autointegration-factor (BAF). The structure and function of the ternary complex formed by LEM-domain protein, nuclear lamina protein and BAF are dependent on each other. Moreover, the network formation based on this structure and function is critical for the development of basic biological processes of nuclear, and it plays important roles in chromatin separation in late metaphase and anaphase, NE reassembly after mitosis, morphological maintenance of nuclear and NE in interphase, regulation of DNA replication and DNA damage repair, regulation of gene expression and signaling pathway, and infection of retrovirus. Mutations in genes encoding LEM family proteins have important impacts on development and progression of laminopathic diseases and tumorigenesis. This review provides a detailed summary of structural and functional studies of the LEM family proteins.

Key words: LEM-domain protein, lamina, BAF, mitosis, NE disassembly and reassembly, laminopathies, tumorigenesis

胡清霞,高昂,曾炜佳,王妍馨,董金堂,朱正茂. 高等哺乳动物LEM结构域蛋白家族的研究进展[J]. 遗传, 2015, 37(2): 128-139.

Qingxia Hu,Ang Gao,Weijia Zeng,Yanxin Wang,Jintang Dong,Zhengmao Zhu. Structure and biological functions of mammalian LEM-Domain proteins[J]. HEREDITAS(Beijing), 2015, 37(2): 128-139.

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高等哺乳动物LEM结构域蛋白家族的研究进展

Structure and biological functions of mammalian LEM-Domain proteins

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