遗传 ›› 2020, Vol. 42 ›› Issue (6): 519-523.doi: 10.16288/j.yczz.20-125

• 前沿聚焦 •    下一篇

Prime editing引导植物基因组精确编辑新局面

秦瑞英, 魏鹏程()   

  1. 安徽省农业科学院水稻研究所,安徽省水稻遗传育种重点实验室,合肥 230031
  • 收稿日期:2020-05-02 修回日期:2020-05-20 出版日期:2020-06-20 发布日期:2020-05-28
  • 通讯作者: 魏鹏程 E-mail:weipengcheng@gmail.com
  • 作者简介:秦瑞英,硕士,副研究员,研究方向:作物遗传与育种。E-mail: laorui_1023@163.com
  • 基金资助:
    国家转基因专项资助编号(2019ZX08010003-001-008);国家转基因专项资助编号(2016ZX08010-002-008)

Prime editing creates a novel dimension of plant precise genome editing

Ruiying Qin, Pengcheng Wei()   

  1. Rice Institute, Anhui Academy of Agriculture Science, Anhui Key Laboratory of Rice Genetics and Breeding, Hefei 230031, China
  • Received:2020-05-02 Revised:2020-05-20 Online:2020-06-20 Published:2020-05-28
  • Contact: Wei Pengcheng E-mail:weipengcheng@gmail.com
  • Supported by:
    Supported by the Genetically Modified Breeding Major Projects Nos(2019ZX08010003-001-008);Supported by the Genetically Modified Breeding Major Projects Nos(2016ZX08010-002-008)

摘要:

由于植物细胞内同源重组频率较低、供体传递受限等原因,对植物基因组进行精准编辑十分困难。近期,中国科学院遗传与发育生物学研究所高彩霞团队构建了适用于植物的引导编辑器(plant prime editor, PPE)系统,并在重要作物水稻和小麦中完成了引导编辑。该系统不产生DNA双链断裂,仍可高度准确实现所有可能的12种单碱基替换、多碱基替换及片段缺失插入,从而为植物基因组精确编辑提供了多用途工具。本文介绍了PPE的组成结构和编辑能力,同时也结合其他研究组随后发表的报告综述了植物引导编辑器的优化探索,为合理使用PPEs和继续开展优化工作提供帮助。

关键词: 引导编辑, 精确编辑, CRISPR, 基因编辑, 作物

Abstract:

The precise genome editing has not been well established in plants, largely because of the limited frequency of homology recombination and the delivery barrier of donor templates. Recently, Dr. Caixia Gao’s group from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, developed a series of plant prime editors (PPEs), which mediats the prime editing in the genomes of rice and wheat. The PPE systems are able to generate all 12 kinds of programmable base substitutions, as well desired multiplex nucleotide substitutions and small deletions or insertions without DNA double-strand breaks, thus providing versatile tools for precise plant genome editing. Herein, we introduce the structure and the editing capacity of the PPEs. The attemp on efficiency enhancements of PPEs and other PPEs are also discussed, which may provide a reference for appropriate application of PPEs in plants and also for continuous optimization of the editing tools.

Key words: prime editing, precise editing, CRISPR, genome editing, crop