1RS-7DS.7DL小麦-黑麦小片段易位系的鉴定

doi:10.16288/j.yczz.14-358

遗传 ›› 2015, Vol. 37 ›› Issue (6): 590-598.doi: 10.16288/j.yczz.14-358

1RS-7DS.7DL小麦-黑麦小片段易位系的鉴定

李俊¹, 朱欣果^{1, 2}, 万洪深¹, 王琴¹, 唐宗祥², 符书兰², 杨足君³, 杨漫宇¹, 杨武云¹

1. 四川省农业科学院作物研究所,农业部西南地区小麦生物学与遗传育种重点实验室,成都 610066;
2. 四川农业大学农学院,四川农业大学植物遗传育种省级重点实验室,温江 611130;
3. 电子科技大学生命科学与技术学院,成都 610054

收稿日期:2014-10-19 修回日期:2015-03-06 出版日期:2015-06-20 发布日期:2015-03-26
通讯作者: 杨武云,博士,研究员,研究方向：小麦遗传育种。E-mail: yangwuyun@126.com
作者简介:李俊,博士,副研究员,研究方向：小麦资源创制与遗传育种。Tel: 028-84504259;E-mail: lijunchd@126.com朱欣果,硕士,研究方向：作物推广。E-mail: zhuxingguo5299@126.com李俊和朱欣果为并列第一作者。
基金资助:
国家科技支撑计划项目(编号：2013BAD01B02-7),四川省科技计划项目(编号：2011NZ0098-16,2014TD0014)和四川省财政基因工程项目(编号：2011QNJJ-007)资助

Identification of the 1RS-7DS.7DL wheat-rye small segment translocation lines

Jun Li¹, Xinguo Zhu^{1, 2}, Hongshen Wan¹, Qin Wang¹, Zongxiang Tang², Shulan Fu², Zujun Yang³, Manyu Yang¹, Wuyun Yang¹

1. Key Laboratory of Biology and Genetic Breeding in Wheat (Southwest) of Ministry of Agriculture, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;
2. Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University,Wenjiang 611130, China;
3. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China

Received:2014-10-19 Revised:2015-03-06 Online:2015-06-20 Published:2015-03-26

摘要/Abstract

摘要： 黑麦(Secale cereale L., RR)是改良普通小麦(Triticum aestivum L., AABBDD)的重要基因资源,将黑麦优异基因转移到普通小麦中,是小麦品种改良的有效途经之一。文章将四川地方品种蓬安白麦子(T. aestivum L., AABBDD) 与秦岭黑麦(S. cereale cv. Qinling, RR)杂交,染色体自动加倍获得八倍体小黑麦CD-13(AABBDDRR);通过顺序FISH和GISH分析,发现该八倍体小黑麦1RS端部与7DS的端部发生相互易位,是一个携带1RS-7DS.7DL小麦-黑麦小片段易位染色体的八倍体小黑麦。利用八倍体小黑麦CD-13与四川推广小麦品种川麦42杂交、连续自交,获得包含60个株系的F₅群体;对F₅群体的58个株系进行GISH和FISH分析发现,其中13个株系含有1RS-7DS.7DL小片段易位染色体。在这13个株系中,株系811染色体数目为2n=6x=42,是稳定的1RS-7DS.7DL小片段易位系;并且1RS特异分子标记和醇溶蛋白分析表明,1RS-7DS.7DL易位染色体1RS小片段的断裂点位于分子标记IB267-IAG95之间,不包含编码黑麦碱蛋白的Sec-1位点;同时1RS-7DS.7DL小片段易位系的千粒重与川麦42相当,远远高于八倍体小黑麦CD-13,对千粒重无负作用。因此,1RS-7DS.7DL小麦-黑麦小片段易位系可作为进一步深入研究1RS小片段上的优异基因及其遗传效应的重要材料。

关键词: 小麦, 黑麦, 1RS-7DS.7DL, 小片段易位

Abstract: Rye (Secale cereale L., RR) is a valuable genetic resource for the improvement of common wheat (Triticum aestivum L., AABBDD). Transferring alien rye genes into wheat by distant hybridization and automatic chromosome doubling is an important and efficient method to boost agronomic traits, disease resistance and widening the gene pool in wheat. In this study, an octoploid triticale CD-13 (AABBDDRR) was obtained via automatic chromosome doubling by crossing landrace Penganbaimaizi (T. aestivum L., AABBDD) and rye “Qinling rye” (S. cereale cv. Qinling, RR). GISH and FISH analyses indicated that CD-13 contained a 1RS-7DS.7DL wheat-rye small segment translocation chromosome. In order to transfer the 1RS-7DS small segment translocation into hexaploid wheat, 58 lines of the F₅ inbred population from the cross CD-13 x Chuanmai 42 were screened for rye chromosome segments by GISH and FISH analyses. The results showed that 13 lines contained the 1RS-7DS.7DL small segment translocation chromosome by reciprocal translocation between 1RS and 7DS. These translocation lines carrying 1RS small rye alien segment were tested for the translocation breakpoints and the presence of a storage protein locus Sec-1. The Sec-1 locus was absent in the line 811, a stable 1RS-7DS.7DL small segment translocation line. The translocation breakpoint of 1RS-7DS.7DL of this line was located in the interval of IB267-IAG95 around the telomere of 1RS chromosome. Thousand-kernel weight of the line 811 was much higher than the parent CD-13, but not significantly different from Chuanmai 42. This indicated that 1RS-7DS.7DL small segment translocation had no negative effect on thousand-kernel weight in the genetic background of Chuanmai 42. The line with 1RS-7DS.7DL translocation chromosomes can be used as a new genetic material for further studies of valuable genes and their genetic effect on 1RS small segment.

Key words: wheat, rye, 1RS-7DS.7DL, small segment translocation

李俊, 朱欣果, 万洪深, 王琴, 唐宗祥, 符书兰, 杨足君, 杨漫宇, 杨武云. 1RS-7DS.7DL小麦-黑麦小片段易位系的鉴定[J]. 遗传, 2015, 37(6): 590-598.

Jun Li, Xinguo Zhu, Hongshen Wan, Qin Wang, Zongxiang Tang, Shulan Fu, Zujun Yang, Manyu Yang, Wuyun Yang. Identification of the 1RS-7DS.7DL wheat-rye small segment translocation lines[J]. HEREDITAS(Beijing), 2015, 37(6): 590-598.

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1RS-7DS.7DL小麦-黑麦小片段易位系的鉴定

Identification of the 1RS-7DS.7DL wheat-rye small segment translocation lines

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