普通野生稻中增产相关QTL的发掘

doi:10.3724/SP.J.1005.2012.00215

遗传 ›› 2012, Vol. 34 ›› Issue (2): 215-222.doi: 10.3724/SP.J.1005.2012.00215

普通野生稻中增产相关QTL的发掘

吴比¹, 韩忠民¹, 李志新², 邢永忠¹

1. 华中农业大学作物遗传改良国家重点实验室, 国家植物基因中心(武汉), 武汉 430070 2. 长江大学农学院, 湖北荆州434025

收稿日期:2011-05-23 修回日期:2011-07-20 出版日期:2012-02-20 发布日期:2012-02-25
通讯作者: 邢永忠 E-mail:yzxing@mail.hzau.edu.cn
基金资助:
国家自然科学基金项目(编号：30971749), 国家重点基础研究发展规划(973计划)项目(编号：2010CB125901)和转基因生物新品种培育重大专项(编号：2009ZX08009-103B)资助

Discovery of QTLs increasing yield related traits in common wild rice

WU Bi¹, HAN Zhong-Min¹, LI Zhi-Xin², XING Yong-Zhong¹

1. National Key Laboratory of Crop Genetic Improvement, National Plant Gene Research Center (Wuhan), Huazhong Agricultural University, Wuhan 430070, China 2. Agricultural College of Yangtze University, Jingzhou 434025, China

Received:2011-05-23 Revised:2011-07-20 Online:2012-02-20 Published:2012-02-25

摘要/Abstract

摘要： 普通野生稻(Oryza Rufipogon)是重要的遗传资源, 发掘其优良等位基因将对水稻遗传改良产生重要影响。文章从以珍汕97为轮回亲本, 普通野生稻为供体的BC₂F₁群体中选择一个与珍汕97表型明显不同的单株BC₂F₁-15, 经过连续自交获得回交重组自交系BC₂F₅群体。均匀分布于12条染色体的126个多态性SSR (Simple sequence repeats) 标记基因型分析, 发现BC₂F₁-15单株在30%的标记位点为杂合基因型; 利用该群体共检测到4个抽穗期、3个株高、4个每穗颖花数、2个千粒重和1个单株产量QTL。在第7染色体RM481-RM2区间, 检测到抽穗期、每穗颖花数和产量QTL, 野生稻等位基因表现增效作用; 其他3个每穗颖花数QTL位点, 野生稻等位基因也均具有增效作用。结果表明野生稻携带有增产相关的等位基因, 这些有利等位基因无疑是水稻遗传改良可资利用的新资源。

关键词: 普通野生稻, 产量性状, QTL, 增效作用

Abstract: Common wild rice (Oryza rufipogon) is an important genetic resource. Discovery of desirable alleles in wild rice will make important contributions to rice genetic improvement. In this study, Zhenshan 97 as the recurrent parent and wild rice as the donor parent were used to develop a BC₂F₁ population. One plant BC₂F₁-15 in the population showed distinct phenotype from Zhenshan 97 was selected to produce a population of BC₂F₅ by continuous self-crossing. The genotype assay of the plant BC₂F₁-15 with 126 polymorphic SSR markers evenly distributed on 12 chromosomes showed that it was heterozygous at 30% of the control marker loci. Four, 3, 4, 2, and 1 QTLs were detected for heading date, plant height, spikelets per panicle, grain weight, and single plant yield in the BC₂F₅ popula-tion, respectively. One QTL region flanked by the marker interval of RM481-RM2 on chromosome 7 had pleiotropic effects on heading date, spikelets per panicle, and grain yield per plant, and the alleles of wild rice increased phenotypic values. At the other 3 QTLs for spikelets per panicle, common wild rice had positive effects. These results clearly showed that com-mon wild rice carried desirable alleles for yield related traits. The favorable alleles from common wild rice are new valuable genes for rice breeding.

Key words: common wild rice, yield traits, QTL, positive effects

吴比，韩忠民，李志新，邢永忠. 普通野生稻中增产相关QTL的发掘[J]. 遗传, 2012, 34(2): 215-222.

WU Bi, HAN Zhong-Min, LI Zhi-Xin, XING Yong-Zhong. Discovery of QTLs increasing yield related traits in common wild rice[J]. HEREDITAS, 2012, 34(2): 215-222.

参考文献

[1] 杨守仁, 张龙步, 陈温福, 徐正进, 王进民. 水稻超高产育种的理论和方法. 中国水稻科学, 1996, 10(2): 115-120.
[2] 邹江石, 吕川根. 水稻超高产育种的实践与思考. 作物学报, 2005, 31(2): 254-258.
[3] 钟代彬, 罗利军, 应存山. 野生稻有利基因转移研究进展. 中国水稻科学, 2000, 14(2): 103-106.
[4] Xiao J, Li J, Grandillo S, Ahn SN, Yuan L, Tanksley SD, McCouch SR. Identification of trait-improving quantita-tive trait loci alleles from a wild rice relative, Oryza rufipogon. Genetics, 1998, 150(2): 899-909.
[5] 李德军, 孙传清, 付永彩, 李晨, 朱作峰, 陈亮, 才宏伟, 王象坤. 利用AB-QTL法定位江西东乡野生稻中的高产基因. 科学通报, 2002, 47(11): 854-858.
[6] Fu Q, Zhang P, Tan L, Zhu Z, Ma D, Fu Y, Zhan X, Cai H, Sun C. Analysis of QTLs for yield-related traits in Yuan-jiang common wild rice (Oryza rufipogon Griff.). J Genet Genomics, 2010, 37(2): 147-157.
[7] Thomson MJ, Tai TH, McClung AM, Lai XH, Hinga ME, Lobos KB, Xu Y, Martinez CP, McCouch SR. Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet, 2003, 107(3): 479-493.
[8] Xie XB, Jin FX, Song MH, Suh JP, Hwang HG, Kim YG, McCouch SR, Ahn SN. Fine mapping of a yield-enhancing QTL cluster associated with transgressive variation in an Oryza sativa ×O. rufipogon cross. Theor Appl Genet, 2007, 116(5): 613-622.
[9] Tan LB, Liu FX, Xue W, Wang GJ, Ye S, Zhu ZF, Fu YC, Wang XK, Sun CQ. Development of Oryza rufipo-gon and O. sativa introgression lines and assessment for yield-related quantitative trait loci. J In-tegr Plant Biol, 2007, 49(6): 871-884.
[10] Jing ZB, Qu YY, Yu C, Pan DJ, Fan ZL, Chen JY, Li C. QTL analysis of yield-related traits using an advanced backcross population derived from common wild rice (Oryza rufipogon L). Mol Plant Breed, 2010, 1(1): 1-10.
[11] Moncada P, Martínez CP, Borrero J, Chatel M, Gauch H Jr, Guimaraes E, Tohme J, McCouch SR. Quantitative trait loci for yield and yield components in an Oryza sativa ×Oryza rufipogon BC₂F₂ population evaluated in an upland environment. Theor Appl Genet, 2001, 102(1): 41-52.
[12] Tian F, Li DJ, Fu Q, Zhu ZF, Fu YC, Wang XK, Sun CQ. Construction of introgression lines carrying wild rice (Oryza rufipogon Griff.) segments in cultivated rice (Oryza sativa L.) background and characterization of introgressed segments associated with yield-related traits. Theor Appl Genet, 2006, 112(3): 570-580.
[13] Tian F, Zhu ZF, Zhang BS, Tan LB, Fu YC, Wang XK, Sun CQ. Fine mapping of a quantitative trait locus for grain number per panicle from wild rice (Oryza rufipogon Griff.). Theor Appl Genet, 2006, 113(4): 619-629.
[14] Yoon DB, Kang KH, Kim HJ, Ju HG, Kwon SJ, Suh JP, Jeong OY, Ahn SN. Mapping quantitative trait loci for yield components and morphological traits in an advanced backcross population between Oryza grandiglumis and the O. sativa japonica cultivar Hwaseongbyeo. Theor Appl Genet, 2006, 112(6): 1052-1062.
[15] Murray MG, Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res, 1980, 8(19): 4321-4326.
[16] Temnykh S, Park WD, Ayres N, Cartinhour S, Hauck N, Lipovich L, Cho YG, Ishii T, McCouch SR. Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theor Appl Genet, 2000, 100(5): 697-712.
[17] McCouch SR, Teytelman L, Xu Y, Lobos KB, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res, 2002, 9(6): 199-207.
[18] Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L. MAPMAKER: an interactive comp

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普通野生稻中增产相关QTL的发掘

Discovery of QTLs increasing yield related traits in common wild rice

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