绵羊发情周期不同组织Cry1 mRNA转录水平相对定量研究

doi:10.3724/SP.J.1005.2013.00085

遗传 ›› 2013, Vol. 35 ›› Issue (1): 85-92.doi: 10.3724/SP.J.1005.2013.00085

绵羊发情周期不同组织Cry1 mRNA转录水平相对定量研究

高磊^1,2, 甘尚权^1,2, 杨井泉^1,2, 杨剑波^1,2, 梁耀伟^1,2, 阿不都艾尼·努拉洪³, 沈敏^1,2

1. 新疆农垦科学院畜牧兽医研究所, 石河子 832000; 2. 新疆兵团绵羊繁育生物技术重点实验室, 石河子 832000; 3. 新疆兵团农三师四十四团兽医站, 图木舒克市 843900;

收稿日期:2012-07-06 修回日期:2012-09-27 出版日期:2013-01-20 发布日期:2013-01-25
通讯作者: 沈敏 E-mail:shenmin0993@sina.com
基金资助:
973计划前期研究专项(编号：2009CB125907), 国家自然科学基金项目(编号：30960249)和兵团种质资源创新与功能基因发掘及利用专项(编号：2012BB044)资助

Relative quantification of mRNA transcription of Cry1 in dif-ferent tissues of sheep in oestrous cycle by real-time quantitative PCR

GAO Lei^1,2, GAN Shang-Quan^1,2, YANG Jin-Quan^1,2, YANG Jian-Bo^1,2, LIANG Yao-Wei^1,2, Abdulla Aini·Nula Hong³, SHEN Min^1,2

1. Animal Husbandry and Veterinary Institute, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China; 2. Key Laboratory of sheep Breeding and Development Technology of Xinjiang Production and Construction Crops, Shihezi 832000, China; 3. Agriculture Third Division forty-four regiment Veterinary Station of Xinjiang Production and Construction Corps, Tumushuke City 843900, China

Received:2012-07-06 Revised:2012-09-27 Online:2013-01-20 Published:2013-01-25

摘要/Abstract

摘要： 研究表明, 时钟基因Cry1在哺乳动物季节性繁殖内分泌过程中可能发挥了重要作用。文章以多浪羊(非季节性繁殖)和中国美利奴羊(季节性繁殖)为研究对象, 采用实时荧光定量PCR技术对下丘脑-垂体-性腺轴主要组织Cry1在发情周期不同阶段的表达变化情况进行了跟踪检测。结果表明：绵羊Cry1在所检测组织中均有表达, 其中松果体和甲状腺Cry1的表达水平较高; 不同绵羊品种Cry1在发情周期不同阶段的组织表达谱基本一致, 除下丘脑外, 卵巢、子宫、松果体、垂体和甲状腺中Cry1的表达水平均是在发情前期达到峰值; 卵巢、子宫、垂体和松果体Cry1在发情前期和发情期的表达变化幅度存在显著的品种间差异。研究结果表明：Cry1可能具有启动发情和季节性繁殖的重要作用。

关键词: Cry1基因, 发情周期, 下丘脑-垂体-性腺轴, 绵羊

Abstract: Studies have shown that clock gene Cry1 may have important roles in the endocrine process of seasonal reproduction in mammals. In this study, Duolang sheep (non-seasonal reproduction sheep breed) and Chinese Merino (seasonal reproduction sheep breed) were used to determine the expression change of Cry1 in hypothala-mus-pituitary-ovary axis in different stage of estrous cycle by quantitative real-time PCR. The results showed that the Cry1 mRNA was expressed in all tested tissues, in which the expression levels in pineal gland and thyroid gland were higher than in other tissues. As far as different sheep breeds were concerned, the tissue expression profiles of Cry1 at different stage of estrous cycle were broadly similar. Besides hypothalamus, the expression levels of Cry1 in ovary, uterus, pineal gland, pituitary gland, and thyroid gland were all reached to peak in proestrus. The differences of expression change extent for Cry1 in vary, uterus, pineal gland, and pituitary gland in proestrus and oestrus were significant between different sheep breeds. The results suggested that Cry1 may play roles in switching on the estrus and seasonal reproduction.

Key words: Cry1 gene, estrous cycle, hypothalamus-pituitary-ovary axis, sheep

高磊，甘尚权，杨井泉，杨剑波，梁耀伟，阿不都艾尼·努拉洪，沈敏. 绵羊发情周期不同组织Cry1 mRNA转录水平相对定量研究[J]. 遗传, 2013, 35(1): 85-92.

GAO Lei GAN Shang-Quan YANG Jin-Quan YANG Jian-Bo LIANG Yao-Wei Abdulla Aini·Nula Hong SHEN Min. Relative quantification of mRNA transcription of Cry1 in dif-ferent tissues of sheep in oestrous cycle by real-time quantitative PCR[J]. HEREDITAS, 2013, 35(1): 85-92.

参考文献

[1] King DP, Takahashi JS. Molecular genetics of circadian rhythms in mammals. Annu Rev Neurosci, 2000, 23(1): 713-742.
[2] Ralph MR, Menaker M. A mutation of the circadian system in golden hamsters. Science, 1988, 241(4870): 1225-1227.
[3] Lincoln G, Messager S, Andersson H, Hazlerigg D. Temporal expression of seven clock genes in the suprachiasmatic nucleus and the pars tuberalis of the sheep: evidence for an internal coincidence timer. Proc Natl Acad Sci USA, 2002, 99(21): 13890-13895.
[4] Miyamoto Y, Sancar A. Vitamin B₂-based blue-light photoreceptors in the retinohypothalamic tract as the photoactive pigments for setting the circadian clock in mammals. Proc Natl Acad Sci USA, 1998, 95(11): 6097-102.
[5] Lincoln GA, Andersson H, Loudon A. Clock genes in calendar cells as the basis of annual timekeeping in mammals-a unifying hypothesis. J Endocrinol, 2003, 179(1): 1-13.
[6] van der Horst GT, Muijtjens M, Kobayashi K, Takano R, Kanno S, Takao M, de Wit J, Verkerk A, Eker AP, van Leenen D, Buijs R, Bootsma D, Hoeijmakers JH, Yasui A. Mammalian Cry1 and Cry2 are essential for maintenance of circadian rhythms. Nature, 1999, 398(6728): 627-630.
[7] Park K, Kang HM. Cloning and circadian expression of rat Cry1. Mol Cells, 2004, 18(2): 256-260.
[8] Hazlerigg DG, Andersson H, Johnston JD, Lincoln G. Mo-lecular characterization of the long-day response in the Soay sheep, a seasonal mammal. Curr Biol, 2004, 14(4): 334-339.
[9] Vitaterna MH, Selby CP, Todo T, Niwa H, Thompson C, Fruechte EM, Hitomi K, Thresher RJ, Ishikawa T, Miya-zaki J, Takahashi JS, Sancar A. Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2. Proc Natl Acad Sci USA, 1999, 96(21): 12114-12119.
[10] Shearman LP, Sriram S, Weaver DR, Maywood ES, Chaves I, Zheng BH, Kume K, Lee CC, van der Horst GTJ, Hastings MH, Reppert SM. Interacting molecular loops in the mammalian circadian clock. Science, 2000, 288(5468): 1013-1019.
[11] 高磊, 甘尚权, 杨井泉, 沈敏. 多浪羊发情周期不同阶段阴道细胞变化观察. 黑龙江畜牧兽医, 2012, (21): 35-37.
[12] Mulsant P, Lecerf F, Fabre S, Schibler L, Monget P, Lanneluc I, Pisselet C, Riquet J, Monniaux D, Callebaut I, Cribiu E, Thimonier J, Teyssier J, Bodin L, Cognié Y, Chitour N, Elsen JM. Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Mérino ewes. Proc Natl Acad Sci USA, 2001, 98(9): 5104-5109.
[13] van der Spek PJ, Kobayashi K, Bootsma D, Takao M, Eker APM, Yasui A. Cloning, tissue expression, and mapping of a human photolyase homolog with similarity to plant blue-light receptors. Genomics, 1996, 37(2): 177-182.
[14] Kobayashi K, Kanno SI, Takao M, Yasui A, Smit B, van der Horst GTJ. Characterization of photolyase/blue-light receptor homologs in mouse and human cells. Nucleic Acids Res, 1998, 26 (22): 5086-5092.
[15] Rataiczak CK, Herzoq ED, Muqlia LJ. Clock gene ex-pression in gravid uterus and extra- embryonic tissues during late gestation in the mouse. Reprod Fertil Dey, 2010, 22(5): 743- 750.
[16] Lincoln GA, Andersson H, Hazlerigg D. Clock genes and the long-term regulation of prolactin secretion: evidence for a photoperiod/circannual timer in the pars tuberalis. J Neuroendocrinol, 2003, 15(4): 390-397.
[17] Johnston JD, Tournier BB, Andersson H, Masson-Pévet M, Lincoln GA, Hazlerigg DG. Multiple effects of melatonin on rhythmic clock gene expression in the mammalian pars tuberalis. Endocrinology, 2006, 147(2): 959-965.
[18] Barrett P, Ebling FJP, Schuhler S, Wilson D, Ross AW, Warner A, Jethwa P, Boelen A, Visser TJ, Ozanne DM, Archer ZA, Mercer JG, Mrgan PJ. Hypothalamic thyroid hormone catabolism acts as a gatekeeper for the seasonal control of body weight and reproduction. Endocrinology, 2007, 148 (8): 3608-36

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

绵羊发情周期不同组织Cry1 mRNA转录水平相对定量研究

Relative quantification of mRNA transcription of Cry1 in dif-ferent tissues of sheep in oestrous cycle by real-time quantitative PCR

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	龚一鸣, 王翔宇, 贺小云, 刘玉芳, 余平, 储明星, 狄冉. 绵羊FecB突变对BMPR1B活性及BMP/SMAD通路的影响研究进展[J]. 遗传, 2023, 45(4): 295-305.
[2]	王海涛, 李亭亭, 黄勋, 马润林, 刘秋月. 遗传修饰技术在绵羊分子设计育种中的应用[J]. 遗传, 2021, 43(6): 580-600.
[3]	何晓红, 蒋琳, 浦亚斌, 赵倩君, 马月辉. 牛、绵羊角的遗传定位及遗传机制研究进展[J]. 遗传, 2021, 43(1): 40-51.
[4]	王冰源, 牟玉莲, 李奎, 刘志国. 农业动物干细胞研究进展[J]. 遗传, 2020, 42(11): 1073-1080.
[5]	赵志达,张莉. 基因组选择在绵羊育种中的应用[J]. 遗传, 2019, 41(4): 293-303.
[6]	胡广东,郝科兴,黄涛,曾维斌,谷新利,王静. 绵羊高效转基因通用型piggyBac转座子载体构建及功能验证[J]. 遗传, 2018, 40(8): 647-656.
[7]	夏青, 刘秋月, 王翔宇, 胡文萍, 李春艳, 贺小云, 储明星, 狄冉. 绵羊季节性繁殖分子机制及休情季节诱导绵羊发情配种技术[J]. 遗传, 2018, 40(5): 369-377.
[8]	赵永欣, 李孟华, 赵要风. 中国绵羊起源、进化和遗传多样性研究进展[J]. 遗传, 2017, 39(11): 958-973.
[9]	王伟, 王玉霜, 黄兰兰, 简子健, 王新华, 刘守仁, 皮文辉. siRNA干扰绵羊胚胎成纤维细胞Lig4基因增加同源重组载体重连修复效率[J]. 遗传, 2016, 38(9): 831-839.
[10]	陈天直, 赵兵令, 刘宇, 赵园园, 王海东, 范瑞文, 王鹏超, 董常生. GPR143在绵羊皮肤组织中的表达及定位分析[J]. 遗传, 2016, 38(7): 658-665.
[11]	高磊,沈敏,甘尚权,杨井泉,张译元. 绵羊CCNG1基因克隆及表达分析[J]. 遗传, 2015, 37(4): 374-381.
[12]	王春玲孟晨玲曹少先张俊孟春花王慧利方永飞朱冬冬茆达干. 湖羊、东湖杂种羊POMC基因外显子3单核苷酸多态性及其与生长性状的关联分析[J]. 遗传, 2013, 35(9): 1095-1100.
[13]	杨小亮，白大章，邱巍，董慧琴，李大全，陈芳，马润林，Hugh T Blair，高剑峰. 以绵羊MHC区段BAC克隆酶切片段为探针杂交筛选绵羊混合组织cDNA文库[J]. 遗传, 2012, 34(7): 887-894.
[14]	杨剑波，甘尚权，杨永林，张红琳，宋天增，冯静，杨井泉，高磊，石国庆，沈敏. 绵羊ILK基因的克隆及其在毛囊生长期的表达[J]. 遗传, 2012, 34(6): 719-726.
[15]	李洪涛何新周志勇赵松华张文祥刘钢赵宗胜贾斌. Slc7a11基因在不同被毛颜色哈萨克羊皮肤组织中的表达分析[J]. 遗传, 2012, 34(10): 1314-1319.