遗传 ›› 2000, Vol. 22 ›› Issue (4): 214-216.
姜运良1;李宁2;习欠云3;吴常信4 JIANG Yun-liang1;LI Ning2;XI Qian-yun3;WU Chang-xin4
摘要: 雌激素受体基因(ESR)是控制猪高产仔数的主效基因之一,具有明显的基因效应:BB型比AA型母猪胎总产仔数和产活仔数分别高出1.40~3.37和0.63~3.58头,是目前商品猪育种和生产中重点检测的主要基因之一。常规采用PCR-RFLPs方法区分该基因由点突变造成的3种不同的基因型。本研究建立1种基于PCR的SSCP(single-stranded conformation polymorphism)方法对猪ESR该位置的点突变进行检测,具有操作简便、灵敏度高和不需要酶切等优点,可以在育种实践中广泛应用。
Abstract:ESR is a major gene controlling litter size in swine.The sows of BB genotype produce 1.40~3.37 more piglets of the total number born and 1.07~2.40 piglets of the number born alive respectively comparing with those of AA genotype.ESR has been one of the widely detected genes in pig breeding and production.Usually the point mutation of ESR gene was detected by PCR-RFLP approach.The present study established a novel method based on PCR-SSCP,with the advantage of easy maniputation,high sensitivity and no necessity for restriction enzyme digestion.This method may be applied for commercial detection of the point mutation of ESR gene in swine breeding.