摘要： 为了探讨白血病抑制因子（LIF）基因在习惯性流产患者子宫蜕膜的表达与其病因的关系，本文利用半定量逆转录聚合酶链反应（RT-PCR）技术，对32例原因不明性习惯性流产患者（病例组）和36例正常妊娠妇女（对照组）子宫蜕膜LIF mRNA进行了研究。结果发现对照组LIF mRNA表达水平为1.314±0.457,病例组表达水平为0.512±0.219，两组相比较，差异有极显著性（P＜0.01）。病例组中有3例患者完全缺乏LIF基因表达。这些研究结果提示 LIF基因表达缺失或减弱，可能是导致原因不明习惯性流产的原因之一，妊娠早期子宫蜕膜LIF基因表达检测可作为诊断原因不明习惯性流产病因的指标之一。
Abstract:To evaluate the relationship beween habitual aborion of unknown etiology and expression of leukemia inhibitory factor(LIF) gene in the decidua,the expression of LIF gene in the decidua were detected in 32 cases of habitual abortion of unknown etiology(abortion group)and 36 cases of normal pregnancy(control group) by reverse transcription-polymerase chain reacion(RT-PCR).The results showed that the level of LIF mRNA expression of the decidua in abortion group was 0.512±0.219,significantly lower than those in the control group(1.314±0.457,P＜0.01).The findings indicated that the low expression of LIF gene in abortion group might be one of the causes of habitual abortion.Analysis on the LIF gene expression of decidua can be used as a index for diagnosis of the habitual abortion.