遗传 ›› 2012, Vol. 34 ›› Issue (10): 1320-1327.doi: 10.3724/SP.J.1005.2012.01320

• 研究报告 • 上一篇    下一篇

鸡视网膜母细胞瘤基因1(RB1)多态性与体重性状的相关性

陈曦1,2, 张慧1,2, 王宇祥1,2, 王守志1,2, 程博涵1,2, 李辉1,2   

  1. 1. 农业部鸡遗传育种重点实验室, 哈尔滨150030 2. 东北农业大学动物科学技术学院, 哈尔滨150030
  • 收稿日期:2012-06-14 修回日期:2012-07-27 出版日期:2012-10-20 发布日期:2012-10-25
  • 通讯作者: 李辉 E-mail:lihui@neau.edu.cn
  • 基金资助:

    现代农业产业技术体系建设专项资金(编号:CARS-42), 国家重点基础研究发展计划(973计划)项目(编号:2009CB941604), 黑龙江省高等学校科技创新团队建设项目(编号:2010td02), 国家高技术研究发展计划(863)项目(编号:2011AA100301)资助

Association of single nucleotide polymorphism of RB1 gene with body weight traits in chicken

CHEN Xi1,2, ZHANG Hui1,2, WANG Yu-Xiang1,2, WANG Shou-Zhi1,2, CHENG Bo-Han1,2, LI Hui1,2   

  1. 1. Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China 2. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
  • Received:2012-06-14 Revised:2012-07-27 Online:2012-10-20 Published:2012-10-25

摘要: 为探讨鸡视网膜母细胞瘤基因1(Retinoblastoma1, RB1)多态性对体重性状的影响, 文章以东北农业大学高、低脂双向选择品系肉鸡为实验材料, 采用MALDI-TOF-MS、PCR-RFLP方法进行基因多态性检测和个体基因型分析, 共获得27个SNP位点的基因型数据。采用滑动窗口法构建单倍型, 进而利用单位点和单倍型分别与鸡体重性状进行关联分析。结合单位点和单倍型分析结果, 确定了RB1基因上4个显著影响1周龄体重的SNP位点, 2个显著影响1、3周龄体重的SNP位点。研究结果表明RB1基因是影响鸡早期体重性状的重要候选基因。

关键词: 鸡, 体重, RB1基因, 多态性, 关联分析

Abstract: The Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) were used in the current study to investigate the effects of Retinoblastoma1 (RB1) gene on chicken body weight (BW). The single nucleotide polymorphisms (SNPs) of the individuals were detected by MALDI-TOF-MS and PCR-RFLP methods and the genotypes of 27 SNPs were obtained. Haplotypes were constructed by liding window approach. Association analysis between single SNPs and haplotypes and body weight were conducted, respectively. Based on the results of single SNPs and haplotype association analyses. Four SNPs with significant effects on body weight at 1 week of age (BW1) and 2 SNPs with significant effects on BW1 and BW3 were determined. These results suggested that RB1 is an important candidate gene that affects chicken early growth and development.

Key words: body weight, RB1 gene, polymorphism, association analysis, chicken