对家蚕第18连锁群隐性基因elp、ch-2和mln测交系的分子定位分析

doi:10.3724/SP.J.1005.2013.00373

遗传 ›› 2013, Vol. 35 ›› Issue (3): 373-378.doi: 10.3724/SP.J.1005.2013.00373

对家蚕第18连锁群隐性基因elp、ch-2和mln测交系的分子定位分析

刘先方¹, 马晓³ , 侯成香², 李冰¹, 李木旺^1,2

1. 江苏科技大学蚕业研究所, 镇江212018 2. 中国农业科学院蚕业研究所, 镇江212018 3. 江苏科技大学生物技术与环境工程学院, 镇江212018

收稿日期:2012-10-11 修回日期:2012-11-21 出版日期:2013-03-20 发布日期:2013-03-25
通讯作者: 李木旺 E-mail:muwang_li@hotmail.com
基金资助:
国家自然科学基金项目(编号：30825007, 30972145), 江苏省自然科学基金项目(编号：BK2009220)和江苏省青蓝工程项目、江苏科技大学研究生科技创新计划资助

Molecular mapping of test mapping strain for 18th linkage group re-cessive genes elp, ch-2 and mln in silkworm (Bombyx mori)

LIU Xian-Fang¹, MA Xiao³, HOU Cheng-Xiang², LI Bing¹, LI Mu-Wang^1,2

1. Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China 2. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China 3. College of Biotechnology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China

Received:2012-10-11 Revised:2012-11-21 Online:2013-03-20 Published:2013-03-25

摘要/Abstract

摘要： 家蚕长形卵(elp)、第二隐性赤蚁(ch-2)、暗化型(mln)均为第18染色体上的隐性突变, 在经典连锁图谱上的顺序和遗传距离已经排定。文章采用正常卵、正常黑蚁及正常白蛾品种P50与包含此3个隐性突变的三隐性测交系W18组配正反交群体, F1回交W18后获得回交群体(P50×W18)♀×W18♂ 和W18♀×(P50×W18)♂, 分别记作BC1F和BC1M, 利用已构建的家蚕SSR分子连锁图谱和根据家蚕基因组精细图设计的STS标记, 对这3个突变基因elp、ch-2、mln进行了分子定位研究, 并根据家蚕基因组精细图, 将第18连锁群的经典遗传图、分子连锁图和基因组物理图进行了对应。整合后的图谱遗传距离为94.2 cM, 突变基因和分子标记的排列顺序分别与形态标记连锁图和基因组精细图相一致, 研究结果对家蚕第18 染色体上其他突变的定位与克隆有重要的借鉴作用。

关键词: 家蚕, 第18连锁群, 突变, 分子标记, 基因定位

Abstract: The ellipsoid egg, the second recessive gene of chocolate larvae, and melanism are controlled by three recessive genes, elp, ch-2, and mln in silkworm, respectively. Their order and genetic distance have been scheduled in established linkage group. Owing to lack of crossing over in females, the reciprocal backcrossed F1(BC1) progenies were bred for linkage analysis using the wild type silkworm strain P50(+elp+ch-2+mln /+elp+ch-2+mln) and W18 with ellipsoid egg, the second recessive gene of chocolate larvae, and melanism (elp ch-2 mln / elp ch-2 mln). In this research, we mapped three mutant genes, elp, ch-2, and mln on the chromosome 18 based on the SSR linkage map and STS markers designed based on silkworm genome sequence. The established linkage group, molecular linkage group, and the physic map of chromosome 18 had been corresponded. The genetic distance for this chromosome in this research was 94.2 cM, and the order of the mutants and molecular markers were consistent with the established silkworm linkage maps and the fine ge-nome sequences. This research will lay important bases for map-based cloning for other mutants on chromosome 18.

Key words: Bombyx mori, 18th chromosome, mutant, molecular marker, gene mapping

刘先方，马晓，侯成香，李冰，李木旺. 对家蚕第18连锁群隐性基因elp、ch-2和mln测交系的分子定位分析[J]. 遗传, 2013, 35(3): 373-378.

LIU Xian-Fang MA Xiao HOU Cheng-Xiang LI Bing LI Mu-Wang. Molecular mapping of test mapping strain for 18th linkage group re-cessive genes elp, ch-2 and mln in silkworm (Bombyx mori)[J]. HEREDITAS, 2013, 35(3): 373-378.

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对家蚕第18连锁群隐性基因elp、ch-2和mln测交系的分子定位分析

Molecular mapping of test mapping strain for 18th linkage group re-cessive genes elp, ch-2 and mln in silkworm (Bombyx mori)

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