玉米籽粒重金属铅(Pb2+)含量的QTL定位

doi:10.3724/SP.J.1005.2014.0821

遗传 ›› 2014, Vol. 36 ›› Issue (8): 821-826.doi: 10.3724/SP.J.1005.2014.0821

玉米籽粒重金属铅(Pb²⁺)含量的QTL定位

赵雄伟, 林海建, 张志明, 沈亚欧, 潘光堂

四川农业大学玉米研究所,农业部西南玉米生物学与遗传育种重点实验室,成都611130

收稿日期:2014-02-15 出版日期:2014-08-20 发布日期:2013-07-19
通讯作者: 潘光堂,博士,教授,研究方向：玉米生物技术与遗传育种。E-mail:pangt@sicau.edu.cn
作者简介:赵雄伟,硕士研究生,专业方向：玉米逆境分子生物学与遗传育种。E-mail:zhaoxw198866@163.com
基金资助:
国家自然科学基金项目(编号：31171567)资助

Mapping of QTLs controlling Pb²⁺ content in maize kernels under Pb²⁺stress

Xiongwei Zhao, Haijian Lin, Zhiming Zhang, Yaou Shen, Guangtang Pan

Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Institute of Sichuan Agricultural University, Chengdu 611130, China

Received:2014-02-15 Online:2014-08-20 Published:2013-07-19

摘要/Abstract

摘要：

铅(Pb²⁺)是现存环境最大量的有毒重金属污染元素,在我国特别是西南地区种植的玉米受重金属Pb²⁺污染日益严重,已严重影响到食物安全。文章利用玉米籽粒Pb²⁺低富集自交系178和籽粒Pb²⁺高富集自交系9782杂交衍生的重组自交系群体为作图群体,利用165对SSR多态性标记,构建了总长度为1499.85 cM、标记间平均距离为9.07 cM的分子遗传图谱,对玉米籽粒Pb²⁺含量性状进行了QTL定位分析,以期为选育籽粒低富集Pb²⁺的玉米新品种提供参考。结果表明,在Pb²⁺浓度为333.32 mg/kg胁迫下,共检测到2个与籽粒Pb²⁺含量相关的QTL,分别位于玉米第1、第4号染色体,其中qPC1位于标记区间umc1661~phi002h之间,表型贡献率为11.13%,加性效应为0.062;qPC4位于umc1117~nc005之间,表型贡献率为5.55%,加性效应为-0.044。性状相关分析结果表明,籽粒中Pb²⁺含量与穗长、穗粗、行粒数、穗重和百粒重等产量性状间均未达到显著水平,表明选育玉米籽粒Pb²⁺低富集的新自交系或杂交种不一定会影响到产量性状,而且籽粒Pb²⁺含量是一个相对独立的遗传性状。

关键词: 玉米, Pb2+胁迫, 籽粒Pb2+含量, QTL定位

Abstract:

Lead (Pb²⁺) is one of the most abundant heavy-metal elements in the environment. Pb²⁺ pollution has become increasingly serious in maize planting areas, especially in the southwest of China, which even threatens food security. In the present study, a RILpopulation derived from 178 (an inbred line with low accumulation of Pb²⁺ in the kernels) and 9782 (a Pb²⁺-hyperaccumulator in the kernels) was used for QTL mapping. A molecular genetic map with the length of 1499.85 cM and an average inter-marker distance of 9.07 cM was constructed with 165 pairs of SSR markers. QTLs controlling Pb²⁺ content in maize kernels were then analyzed to provide the basis for breeding elite maize varieties with low Pb²⁺in the kernels. Two QTLs, qPC1 and qPC4, related to Pb²⁺ content in maize kernels were identified on chromosome 1 and 4, respectively. qPC1 was located between markers umc1661 and phi002, accounting for 11.13% of phenotypic variance with an additive effect value of 0.062. While qPC4 was located between markers umc1117 and nc005, explaining 5.55% of the phenotypic variance with an additive effect value of -0.044. However, there was no significant correlation observed between Pb²⁺ content in the kernels and any of yield-related traits including ear length, ear diameter, kernel row number and weight of per-hundred kernels, indicating that yield-related traits would not be changed in the process of low-Pb²⁺maize breeding. This suggested that the Pb²⁺content in maize kernel under Pb²⁺stress was an independent genetic trait.

Key words: maize ( L.), Pb2+ stress, Pb2+ content in maize kernels, QTLs mapping

赵雄伟, 林海建, 张志明, 沈亚欧, 潘光堂. 玉米籽粒重金属铅(Pb²⁺)含量的QTL定位[J]. 遗传, 2014, 36(8): 821-826.

Xiongwei Zhao, Haijian Lin, Zhiming Zhang, Yaou Shen, Guangtang Pan. Mapping of QTLs controlling Pb²⁺ content in maize kernels under Pb²⁺stress[J]. HEREDITAS(Beijing), 2014, 36(8): 821-826.

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玉米籽粒重金属铅(Pb²⁺)含量的QTL定位

Mapping of QTLs controlling Pb²⁺ content in maize kernels under Pb²⁺stress

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