陆地棉超矮杆突变体基因的初步定位

doi:10.3724/SP.J.1005.2011.01393

遗传 ›› 2011, Vol. 33 ›› Issue (12): 1393-1397.doi: 10.3724/SP.J.1005.2011.01393

陆地棉超矮杆突变体基因的初步定位

景超¹, 马晓杰², 狄佳春², 陈旭升²

1. 南京农业大学农学院, 南京210095 2. 江苏省农业科学院, 农业部长江下游棉花与油菜重点实验室, 南京210014

收稿日期:2011-04-03 修回日期:2011-10-21 出版日期:2011-12-20 发布日期:2011-12-25
通讯作者: 陈旭升 E-mail:njcxs@126.com
基金资助:
转基因生物新品种培育科技重大专项子课题(编号：2008ZX08005-001), 江苏省农业科技自主创新资金项目(编号：CX 09-115)资助

Gene mapping of an ultra-dwarf mutant in upland cotton

JING Chao¹, MA Xiao-Jie², DI Jia-Chun², CHEN Xu-Sheng²

1. Agronomy College of Nanjing Agricultural University, Nanjing 210095, China 2. Key Laboratory of Cotton and Rape in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Jiangsu Academy of Agri-cultural Sciences, Nanjing 210014, China

Received:2011-04-03 Revised:2011-10-21 Online:2011-12-20 Published:2011-12-25

摘要/Abstract

摘要： 陆地棉超矮杆是一种新发现的突变体材料。前期对其遗传规律的研究表明, 超矮杆突变性状是受一对完全隐性基因du控制的质量性状。文章以“超矮1号”和“新陆早16”配置的F₂为作图群体, 通过对双亲以及近等基因池的筛选, 在1 350对SSR引物中共获得70对多态性引物。而后检测F₂作图群体每个单株的基因型, 显示有36个标记可以连锁, 分布在8个连锁群上, 其中超矮杆突变性状位于连锁群LG01。与目标基因du连锁的分子标记有7个：NAU2679、NAU2749、NAU905、NAU2838、NAU5373、NAU2238和NAU4946, 它们皆为共显性标记。依据现有的棉花遗传图谱, 标记NAU4946、NAU2238、NAU905、NAU5373和NAU2679位于第6染色体上, 而目标基因du位于NAU2238和NAU4946之间, 遗传距离分别为3.3 cM和1.4 cM, 由此推定du在第6条染色体上。

关键词: 陆地棉, 超矮杆突变体, 基因定位, SSR

Abstract: A ultra-dwarf mutant was newly found in upland cotton (Gossypium hirsutum L.). This mutant was controlled by single recessive gene du. Seventy pairs of polymorphic primers were selected from 1350 primers, which covered all the identified chromosomes by screening in parents and near-isogenic lines. Gene du was mapped using an F₂ population derived from intraspecific crosses between “Ultra-dwarf 1” and “Xinluzao 16”. Thirty-six primers were distributed in eight linkage groups, and du was linked to LG01. Seven co-dominant markers linked to du were NAU2679, NAU2749, NAU905, NAU2838, NAU5373, NAU2238, and NAU4946. Based on the known genetic map of tetraploid cotton, the markers NAU4946, NAU2238, NAU905, NAU5373, and NAU2679 were located on chromosome 6, and the target gene du was located between NAU2238 and NAU4946 with the genetic distances of 3.3 cM and 1.4 cM, respectively. Hence, gene du was located on chromosome 6.

Key words: upland cotton (G. hirsutum L.), ultra-dwarf mutant, gene location, SSR

景超，马晓杰，狄佳春，陈旭升. 陆地棉超矮杆突变体基因的初步定位[J]. 遗传, 2011, 33(12): 1393-1397.

JING Chao, MA Xiao-Jie, DI Jia-Chun, CHEN Xu-Sheng. Gene mapping of an ultra-dwarf mutant in upland cotton[J]. HEREDITAS, 2011, 33(12): 1393-1397.

参考文献

[1] 嵇怡, 缪曼珉, 陈学好. 植物矮生性状的分子遗传研究进展. 分子植物育种, 2006, 4(6): 753-77l.
[2] Khush GS. Green revolution: preparing for the 21st century. Genome, 1999, 42(4): 646-655.
[3] Korzun V, Röder M, Worland AJ, Börner A. Intrachromosomal mapping of genes for dwarfing (Rht12) and vernalization response (Vrn1) in wheat by using RFLP and microsatellite markers. Plant Breed, 1997, 116(3): 227-232.
[4] Korzun V, Roder MS, Ganal MW, Worland AJ, Law CN. Genetic analysis of the dwarfing gene (Rht8) in wheat, Part I. Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat (Triticum aestivum L.). Theor Appl Genet, 1998, 96(8): 1104-1109.
[5] 张京. 大麦矮杆基因uz的SSR标记. 作物学报, 2003, 29(4): 637-640.
[6] 车俐晓, 陈杰, 展霞, 桑燕, 刘敏, 刘保申. 水稻矮秆突变体sde(t)的遗传分析与基因初步定位. 分子植物育种, 2008, 6(4): 760-764.
[7] 袁运动, 程祝宽, 刘保申, 李传友. 水稻矮秆突变体ipd1的遗传分析及其基因精细定位. 山东农业大学学报 (自然科学版), 2008, 40(1): 1-6.
[8] 梅德圣, 王汉中, 李云昌, 胡琼, 李英德, 徐育松. 甘蓝型油菜矮杆突变材料99CDAM的发现及遗传分析. 遗传, 2006, 28(7): 851-857.
[9] 姜亮, 郭龙彪, 姜华, 曾大力, 胡江, 胡立文, 高振宇, 钱前. 一个矮杆叶尖枯水稻突变体的遗传分析和精细定位. 遗传学报, 2008, 35(12): 715-721.
[10] Harland SC. On the genetics of crinkled dwarf rogues in Sea Island cotton. West Ind Bull, 1918, 16(1): 82.
[11] Harland SC. The genetics of cotton. Part IX. Further experience on the inheritance of the crinkled dwarf mutant of G. barbadense. L. in interspecific cross and their bearing on Fisher theory of dominance. J Genet, 1933, 28(2): 315-325.
[12] Hutchinson JB, Ghose RU. On the occurrence of “crinkled dwarf” in Gossypium hirsutum. L. J Genet, 1937, 34(3): 437-446.
[13] Abzalov MF, Zhumaev FK. Revelation in the cotton plant of chromosomes responsible for the regulation of plant growth. Chem Natl Compounds, 1995, 31(1): 111-113.
[14] 何鉴星, 孙传渭, 姜茹琴, 王志龙, 张欣雪, 李爱国, 李赠书, 赵国忠. 棉花矮化突变体的遗传分析. 遗传学报, 1996, 23(5): 397-402.
[15] 陈旭升. 棉花矮杆突变体. 中国棉花, 2004, 31(1): 269.
[16] 陈旭升, 狄佳春, 许乃银, 肖松华, 刘剑光. 陆地棉超矮杆突变性状质量遗传规律分析. 遗传, 2007, 29(4): 471-474.
[17] Paterson AH, Brubaker C, Wendel JF. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol Rep, 1993, 11(2): 122-127.
[18] 张天真, 袁有禄, 郭旺珍, Yu J, Kohel RJ. 棉花高强纤维QTLs的微卫星标记筛选. 中国农业科学, 2001, 34(4): 363-366.
[19] 张军, 武耀廷, 郭旺珍, 张天真. 棉花微卫星标记的PAGE/银染快速检测. 棉花学报, 2000, 12(5): 267-269.
[20] Michelmore RW, Paran L, Kesseli RV. Identification of markers linked to disease-resistance genes by bulked seg-regant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88(21): 9828-9832.
[21] Guo WZ, Cai CP, Wang CB, Han ZG, Song XL, Wang K, Niu XW, Wang C, Lu KY, Shi B, Zhang TZ. A microsa-tellite-based, gene-rich linkage map reveals genome structure, function and evolution in Gossypium. Ge-netics, 2007, 176(1): 527-541.

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陆地棉超矮杆突变体基因的初步定位

Gene mapping of an ultra-dwarf mutant in upland cotton

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