陈绍荣;吕应堂;杨弘远 CHEN Shao-Rong;LU Ying-Tang;YANG Hong-Yuan
摘要： 利用RNA原位杂交技术, 对水稻受精前后雌蕊组织切片进行磷酸化酶基因表达的定位。结果显示, 磷酸化酶mRNA在柱头、花柱、子房壁以及维管束中大量表达, 而胚珠中除合点部位外表达很弱。受精前后的胚囊中各细胞磷酸化酶mRNA表达也很弱, 并且没有明显的时空变化。在积累淀粉的胚乳细胞中磷酸化酶mRNA大量分布。原胚中的磷酸化酶mRNA表达较弱, 到分化胚积累过渡性淀粉时才显著增加。本文首次对磷酸化酶mRNA在植物雌性器官发育过程中的时空分布动态作了初步研究。
Abstract:Phosphorylase gene expression was localized in tissue sections of rice pistils before and after fertilization byin situRNA hybridization. Phosphorylase mRNA was substantially expressed in the stigma, style, ovary wall and vascular bundle, but weakly in ovular tissues except chalazal portion. It was weakly expressed and did not show temporal and spatial changes in embryo sacs before and after fertilization. A great quantity of phosphorylase mRNA was distributed in endosperm cells accumulating starch grains. Phosphorylase mRNA was less in proembryos, but significantly increased in differentiation embryos accumulating transition starch. This report is, for the first time, a tentative investigation on the temporal and spatial expression patterns of phosphorylase mRNA in plant female organ development.