遗传 ›› 2000, Vol. 22 ›› Issue (4): 214-216.

• 论文 • 上一篇    下一篇

猪雌激素受体基因(ESR)点突变的PCR-SSCP检测

姜运良1;李宁2;习欠云3;吴常信4 JIANG Yun-liang1;LI Ning2;XI Qian-yun3;WU Chang-xin4   

  1. 1.山东农业大学动物科技学院,泰安 271018 2.中国农业大学农业生物技术国家重点实验室,北京 100094 3.江西农业大学动物科技学院,南昌 330045 4.中国农业大学动物科技学院,北京 100094 1.College of Animal Science and Technology,Shandong Agricultural University,Taian 271018,China 2.National Laboratory for Agro?biotechnology,China Agricultural University,Beijing 100094,China 3.College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China 4.College of Animal Science and Technology,China Agricultural University,Beijing 100094,China
  • 收稿日期:1900-01-01 出版日期:2000-08-10 发布日期:2000-08-10

Detection of Point Mutation of Porcine Estrogen Receptor Gene(ESR) by PCR-SSCP Approach

  • Received:1900-01-01 Online:2000-08-10 Published:2000-08-10

摘要: 雌激素受体基因(ESR)是控制猪高产仔数的主效基因之一,具有明显的基因效应:BB型比AA型母猪胎总产仔数和产活仔数分别高出1.40~3.37和0.63~3.58头,是目前商品猪育种和生产中重点检测的主要基因之一。常规采用PCR-RFLPs方法区分该基因由点突变造成的3种不同的基因型。本研究建立1种基于PCR的SSCP(single-stranded conformation polymorphism)方法对猪ESR该位置的点突变进行检测,具有操作简便、灵敏度高和不需要酶切等优点,可以在育种实践中广泛应用。
Abstract:ESR is a major gene controlling litter size in swine.The sows of BB genotype produce 1.40~3.37 more piglets of the total number born and 1.07~2.40 piglets of the number born alive respectively comparing with those of AA genotype.ESR has been one of the widely detected genes in pig breeding and production.Usually the point mutation of ESR gene was detected by PCR-RFLP approach.The present study established a novel method based on PCR-SSCP,with the advantage of easy maniputation,high sensitivity and no necessity for restriction enzyme digestion.This method may be applied for commercial detection of the point mutation of ESR gene in swine breeding.

关键词: 猪, ESR基因, pig, PCR-SSCP
Key words,
点突变