遗传 ›› 1996, Vol. 18 ›› Issue (1): 15-18.

• 论文 • 上一篇    下一篇

五种野生稻叶绿体DNA多态性研究

张尚宏 ZHANG Shang-Hong   

  1. 中山大学生物工程研究中心, 广州 510275 Biotechnology Research Center,Zhongshan University,Guangzhou 510275
  • 收稿日期:1900-01-01 出版日期:1996-02-10 发布日期:1996-02-10

Restriction Fragment Analysis of the Chloroplast DNA of 5 WildRice Species

  • Received:1900-01-01 Online:1996-02-10 Published:1996-02-10

摘要: 对野生稻 5个种的18个材料的叶绿体DNA(cpDNA)进行了EcoRI的RFLP分析。
结果显示,共有10种酶切模式,不同种野生稻的cpDNA的RFLP类型都不同,而且在其中一些
种内也有变化,尤以O.rufipogon的种内多态性最为显著,并主要与地理来源有关。本研究还在O.punctata的材料中发现一种以往的分析都不曾描述过的多态性模式。通过对结果的分析,探讨了不同种类野生稻的叶绿体基因组之间以及它们与核基因组之间的进化关系。
Abstract:The polymorphisms of chloroplast DNA from 18 materials of 5 wild rice species were investigated using RFLP analysis.10 restriction patterns were obtained from the analysis of these materials.Different species had different of its RFLP patterns chloroplast DNA,and the polymorphisms existed even with species,especially in O.rufipogon varieties of different geographical origins.In O.punctata a new type of rice chloroplast DNA restriction pattern was discovered which had not been reported before.According to the results obtained,the evolutionary relationships among chlorplast genomes,and between chloroplast and nuclear genomes in different wild rice are discussed.

关键词: 叶绿体DNA, Oryza, RFLP
Key words,
野生稻, 稻属, 小麦染色体RAPD标记, RAPD分析, Langdon二体代换系, 小麦

Key words: Wheat RAPD assay Langdon disomic substitution lines RAPD markers for wheat chromosome