绿色杜氏藻不同β-胡萝卜素羟化酶基因家族胁迫应答模式研究

doi:10.16288/j.yczz.16-327

遗传 ›› 2017, Vol. 39 ›› Issue (2): 156-165.doi: 10.16288/j.yczz.16-327

绿色杜氏藻不同β-胡萝卜素羟化酶基因家族胁迫应答模式研究

朱帅旗(),龚一富(),章丽,俞凯,王何瑜,严小军

宁波大学海洋学院,宁波 315211

收稿日期:2016-09-22 修回日期:2016-12-04 出版日期:2017-02-17 发布日期:2017-02-10
作者简介:朱帅旗,在读硕士研究生,专业方向：藻类生物化学与分子生物学。E-mail: ljlzj2009@163.com|龚一富,博士,副教授,研究方向：植物生物化学与分子生物学。E-mail: gongyifu@163.com
基金资助:
浙江省科技厅重点科技创新团队项目(2012R10029-07, 2010R50029);宁波科技攻关项目(2013C10018);宁波大学优秀学位论文培育基金资助项目(py2014020)

Expression analysis of different β-carotenoid hydroxylase gene families in stress response in Dunaliella viridis

Zhu Shuaiqi(),Gong Yifu(),Zhang Li,Yu Kai,Wang Heyu,Yan Xiaojun

School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received:2016-09-22 Revised:2016-12-04 Online:2017-02-17 Published:2017-02-10
Supported by:
Key Scientific and Technological Innovation Team Project of Zhejiang Province Department of Science and Technology(2012R10029-07, 2010R50029);Foundation of Ningbo Science and Technology(2013C10018);the Outstanding (Postgraduate) Dissertation Growth Foundation of Ningbo University(py2014020)

摘要/Abstract

摘要：

β-胡萝卜素羟化酶(β-carotenoid hydroxylase, CHYB)是植物类胡萝卜素生物合成途径中的一个重要限速酶。本研究对绿色杜氏藻转录组测序数据进行分析,获得2条β-胡萝卜素羟化酶家族基因chyb1和chyb2。采用染色体步移法分别克隆并获得了绿色杜氏藻chyb1和chyb2基因的启动子序列,全长分别为1080 bp(GenBank登录号：KY012338)和1155 bp (GenBank登录号：KY012339)。利用Plantcare软件分析两个启动子的顺式作用元件,结果表明绿色杜氏藻chyb1基因启动子含有与甲基茉莉酸、花生四烯酸、水杨酸等非生物胁迫相关的顺式作用元件,而绿色杜氏藻chyb2基因启动子含有与光照相关的顺式作用元件。通过qRT-PCR分析了绿色杜氏藻CHYB基因家族在不同胁迫下的基因表达水平,结果表明该基因家族的基因表达水平与启动子调控相关,且不同的家族基因应答不同的胁迫。

关键词: 绿色杜氏藻, β-胡萝卜素羟化酶, 基因家族, 启动子, 胁迫

Abstract:

β-carotenoid hydroxylase (CHYB) is an important rate-limiting enzyme in the biosynthesis of plant carotenoid. In this study, chyb1 and chyb2, two gene families in Dunaliella viridis were obtained by RNA-seq. The fragment of promoters of CHYB family genes, 1 080 bp for chyb1 (GenBank No. KY012338) and 1 155 bp for chyb2 (GenBank No. KY012339) were cloned by the Genome Walking Technology, respectively. Cis-acting elements of two promoters were analyzed by Plantcare soft. The results show that the chyb1 gene promoter contains more cis-acting elements in responses to abiotic stresses, such as methyl jasmonate, arachidonic acid, acetylsalicylic acid, and so on. On the other hand, the chyb2 promoter contains more cis-acting elements in response to light stress. qRT-PCR results show that the mRNA expression levels of CHYBs are modulated by their promoters, and different CHYB gene families response to distinct stresses.

Key words: Dunaliella viridis, β-carotenoid hydroxylase, gene family, promoter, stress

朱帅旗,龚一富,章丽,俞凯,王何瑜,严小军. 绿色杜氏藻不同β-胡萝卜素羟化酶基因家族胁迫应答模式研究[J]. 遗传, 2017, 39(2): 156-165.

Zhu Shuaiqi,Gong Yifu,Zhang Li,Yu Kai,Wang Heyu,Yan Xiaojun. Expression analysis of different β-carotenoid hydroxylase gene families in stress response in Dunaliella viridis[J]. Hereditas(Beijing), 2017, 39(2): 156-165.

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绿色杜氏藻不同β-胡萝卜素羟化酶基因家族胁迫应答模式研究

Expression analysis of different β-carotenoid hydroxylase gene families in stress response in Dunaliella viridis

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