lncRNA TCONS_00815878对猪骨骼肌卫星细胞分化的影响

doi:10.16288/j.yczz.19-146

遗传 ›› 2019, Vol. 41 ›› Issue (12): 1119-1128.doi: 10.16288/j.yczz.19-146

lncRNA TCONS_00815878对猪骨骼肌卫星细胞分化的影响

黄子莹¹, 李龙¹, 李倩倩¹, 刘向东^1,², 李长春^1,²()

^1. 华中农业大学,农业动物遗传育种与繁殖教育部重点实验室,武汉 430070
^2. 广西扬翔股份有限公司生产中心,贵港 537131

收稿日期:2019-05-21 修回日期:2019-11-19 出版日期:2019-12-20 发布日期:2019-12-05
通讯作者: 李长春 E-mail:lichangchun@mail.hzau.edu.cn
作者简介:黄子莹,在读硕士研究生,专业方向：动物遗传育种大数据分析。E-mail: 327620138@qq.com
基金资助:
国家自然科学基金项目编号(31872322);中央高校创新研究基金项目编号资助(2662017PY030)

The effect of lncRNA TCONS_00815878 on differentiation of porcine skeletal muscle satellite cells

Ziying Huang¹, Long Li¹, Qianqian Li¹, Xiangdong Liu^1,², Changchun Li^1,²()

^1. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China
^2. Guangxi Yangxiang Co., Ltd. Production Center, Guigang 537131, China

Received:2019-05-21 Revised:2019-11-19 Online:2019-12-20 Published:2019-12-05
Contact: Li Changchun E-mail:lichangchun@mail.hzau.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China No(31872322);Central University Innovation Research Fund No(2662017PY030)

摘要/Abstract

摘要：

猪骨骼肌发育是一个复杂的生物学过程,其中骨骼肌卫星细胞分化是影响骨骼肌发育的重要环节。近年来发现长链非编码RNA (long non-coding RNA, lncRNA)在骨骼肌卫星细胞分化中具有重要作用。为探究lncRNA TCONS_00815878对猪骨骼肌卫星细胞分化的影响,本研究利用qRT-PCR技术检测出生7 d内大白仔猪6种组织(心脏、脾脏、肺脏、肾脏、背肌和腿肌)及从胚胎期到出生后5个不同时间点(35 d、45 d、55 d胚胎及产后第7 d和第200 d后腿肌肉组织) TCONS_00815878的表达情况;利用反义核苷酸(antisense oligonucleotides, ASO)在猪骨骼肌卫星细胞中敲低TCONS_00815878,检验分化标记基因MyoD、MyoG和MyHC表达情况;通过生物信息学分析预测TCONS_00815878靶基因,并利用DAVID软件在线预测其靶基因的功能与通路。结果表明：TCONS_00815878在猪心肌和腿肌中高表达;仔猪出生后7 d内,TCONS_00815878在猪肌肉组织中表达量不断升高,第7 d达到高峰;在猪骨骼肌卫星细胞增殖和分化过程中,TCONS_00815878在分化期表达量不断上升,且在分化30 h表达量达到峰值;敲低TCONS_00815878后,MyoD、MyoG和MyHC基因表达量降低,其中MyoD表达量显著下降(P<0.05)。此外,功能预测结果发现,其靶基因富集到糖酵解和丙酮酸代谢等与骨骼肌卫星细胞分化相关的多个生物学过程。本研究推测,lncRNA TCONS_00815878可能对猪骨骼肌卫星细胞的分化起促进作用。

关键词: lncRNA TCONS_00815878, 猪骨骼肌卫星细胞, 分化, 敲低, 靶基因

Abstract:

Porcine skeletal muscle development is a complex biological process, and differentiation of skeletal muscle satellite cells is an important part of skeletal muscle development. In recent years, it has been found that lncRNA plays an important role in the differentiation of skeletal muscle satellite cells. Here we investigate the effect of lncRNA TCONS_00815878 on the differentiation of porcine skeletal muscle satellite cells. We first used qRT-PCR to detect the expression levels of TCONS_00815878 in six tissues (heart, spleen, lung, kidney, back muscles and leg muscles) of Yorkshire piglets within seven days of birth. At the same time, the expression levels of TCONS_00815878 at five different time points from the embryonic stage to the postnatal stage (35 d, 45 d, 55 d of embryos, and 7 d, 200 d of postpartum leg muscles) were examined. The expression of the differentiation marker genes MyoD, MyoG and MyHC was examined by knocking down TCONS_00815878 in porcine skeletal muscle satellite cells using antisense oligonucleotides (ASO). The target gene of TCONS_00815878 was predicted by bioinformatics analysis, and the function and pathway of its target gene were predicted online using DAVID software. The results showed that TCONS_00815878 had the highest expression level in pig myocardium and leg muscles. Within seven days after birth, TCONS_00815878 increased in the muscle tissue of pigs, and reached the peak of expression level on the 7th day. During the process of proliferation and differentiation of porcine skeletal muscle satellite cells, the expression level of TCONS_00815878 increased during the differentiation stage and peaked at 30 h of differentiation. After knocking down TCONS_00815878, the expression levels of MyoD, MyoG and MyHC were decreased, but the expression level of MyoD was significantly decreased (P<0.05). In addition, functional predictions revealed that the target gene of TCONS_00815878 is enriched in multiple biological processes, such as glycolysis and pyruvate metabolism, related to skeletal muscle satellite cell differentiation. This study speculates that lncRNA TCONS_00815878 may promote the differentiation of porcine skeletal muscle satellite cells.

Key words: lncRNA TCONS_00815878, porcine skeletal muscle satellite cells, differentiation, knockdown, target gene

黄子莹, 李龙, 李倩倩, 刘向东, 李长春. lncRNA TCONS_00815878对猪骨骼肌卫星细胞分化的影响[J]. 遗传, 2019, 41(12): 1119-1128.

Ziying Huang, Long Li, Qianqian Li, Xiangdong Liu, Changchun Li. The effect of lncRNA TCONS_00815878 on differentiation of porcine skeletal muscle satellite cells[J]. Hereditas(Beijing), 2019, 41(12): 1119-1128.

图/表 7

表1

图1

图2

图3

图4

附表1

靶基因列表"

基因名称	基因名称	基因名称	基因名称	基因名称	基因名称
ARFGAP3	ADGRL4	POLR3K	SLC25A26	LAMC2	ARHGEF10
IFT27	PGM1	KCTD5	MITF	INTS7	PLXNA3
PRPF40B	KIF2C	ADRA2B	GXYLT2	LPGAT1	REEP1
IGFBP6	SMOC2	MAP4K4	PPARG	HHAT	TECTA
NCKAP1L	CNKSR3	TBC1D8	GK5	MGAT5	SLC16A3
STAT2	NT5DC1	CHST10	P2RY14	ADGRA2	ADAMTS2
BAZ2A	SNAP91	ANKRD23	SENP5	HERC2	ELOVL5
RASSF8	PSTPIP2	SMYD1	PARP14	SCN3A	CES3
KCNJ8	CTIF	TMSB10	CD47	CALCRL	KDM4B
P3H3	ZNF106	DOK1	OTC	TFPI	BBS9
GAPDH	TYRO3	DGUOK	ATP6AP2	TMEFF2	MPI
TNFRSF1A	RPAP1	PREB	SLC9A7	PLCL1	PPIP5K2
CRACR2A	ATP10A	NBAS	WDR45	CARF	GREB1
ITFG2	ZNF516	CD38	SYNJ2	PARD3B	PTPN6
BID	SERPINB2	KLHL2	PGK1	BARD1	PXDC1
SLC22A23	DPP8	GUCY1B1	ZMAT1	CYP27A1	KDSR
SERPINB1	ARF6	SLC4A4	ELF4	PRKAG3	ST6GAL1
JARID2	KANK1	ARHGAP10	ENOX2	GPC1	NOA1
PHF1	NPR2	ANXA5	FMR1	HDLBP	IGFBP5
VEGFA	TMEM8B	STPG2	TMEM185A	SNED1	SRPK2
ZXDC	ZCCHC7	MAPK10	RENBP	NEB	SLC25A40
TMEM266	FKTN	PAQR3	NADSYN1	CAV2	SCAP
LINGO1	OLFML2A	FGF9	BRMS1	DBNL	ACSM5
EDC3	DNM1	EBPL	EHBP1L1	ZNF282	PADI2
AKAP5	EXD3	PHF11	MAP4K2	CDK3	DCUN1D2
GALNT16	FBXL6	MPHOSPH8	MARK2	GALK1	CARHSP1
IFT43	MYC	DNAH10	DTX4	TANC2	B4GALT2
VASH1	NCOA2	TMEM120B	KBTBD4	FKBP10	CTC1
IRF2BPL	SCYL3	PPTC7	ABTB2	TBKBP1	LMAN2L
SETD3	F5	ACAD10	LMO2	TSPOAP1	CASP10
WDR20	PBX1	HSPB8	MPPED2	ABR	STK36
MBTPS1	CD84	NOS1	TEAD1	ANKFY1	BDKRB2
THAP11	SLAMF8	MIF	SCAMP4	SMTNL2	MED31
SLC9A5	SYT11	LZTR1	ANKRD24	ENO3	CENPH
YIF1B	CERS2	DGCR2	CHAF1A	BCL6B	PANK4
ARHGEF1	FMO5	SIPA1L2	PNPLA6	MYH8	ESYT3
IRF2BP1	ZNF697	ARID5B	ZNF608	SLC5A10	OBSL1
PLEKHA4	TTF2	SPOCK2	PDLIM4	ARHGAP23	CASP8
BCAT2	CTTNBP2NL	CEP55	IL5	PFKM	FLYWCH1
BAX	ARHGAP29	TBC1D12	TRPC7	OXTR	VPS37C
CACNG6	GLMN	SORBS1	FZD4	KCNA5	PARVA
PUSL1	TGFBR3	ZDHHC6	ARHGAP42	CEP19	PHACTR4
MMEL1	GBP2	EIF3A	HINFP	DUS2	RASIP1
CLCN6	HSPA12B	PPP1R12B	SORL1	DIS3L2	OLFM1
NPPA	TTI1	RPP25L	NTM	HS6ST1	CCDC112
VPS13D	KCNB1	CACNB2	PRELP	STK38L	HS1BP3
DHRS3	GUSB	CREM	SRI	WDR73	AHR
FHAD1	CTF1	TRAK1	CALCR	IL20RB	TBC1D23
HMGN2	SETD1A	PTH1R	CROT	CALM3	LYSMD4
EPB41	REXO5	TEX264	PHTF2	WDR4	PDIK1L
CLSPN	ZNF174	NISCH	LAMB1	NFIA
MOCOS	RHBDF1	GLT8D1	KIAA1614	TIA1

附表1

附图1

参考文献 30

[1]	Groenen MAM, Archibald AL, Uenishi H, Tuggle CK, Takeuchi Y, Rothschild MF, Rogel-Gaillard C, Park C, Milan D, Megens HJ, Li ST, Larkin DM, Kim H, Frantz LAF, Caccamo M, Ahn H, Aken BL, Anselmo A, Anthon C, Auvil L, Badaoui B, Beattie CW, Bendixen C, Berman D, Blecha F, Blomberg J, Bolund L, Bosse M, Botti S, Bujie Z, Bystrom M, Capitanu B, Silva DC, Chardon P, Chen C, Cheng R, Choi SH, Chow W, Clark RC, Clee C, Crooijmans RPMA, Dawson HD, Dehais P, De Sapio F, Dibbits B, Drou N, Du ZQ, Eversole K, Fadista J, Fairley S, Faraut T, Faulkner GJ, Fowler KE, Fredholm M, Fritz E, Gilbert JGR, Giuffra E, Gorodkin J, Griffin DK, Harrow JL, Hayward A, Howe K, Hu ZL, Humphray SJ, Hunt T, Hornshøj H, Jeon JT, Jern P, Jones M, Jurka J, Kanamori H, Kapetanovic R, Kim J, Kim JH, Kim KW, Kim TK, Larson G, Lee K, Lee KT, Leggett R, Lewin HA, Li YR, Liu WS, Loveland JE, Lu Y, Lunney JK, Ma J, Madsen O, Mann K, Matthews L, McLaren S, Morozumi T, Murtaugh MP, Narayan J, Nguyen DT, Ni PX, Oh SJ, Onteru S, Panitz F, Park EW, Park HS, Pascal G, Paudel Y, Perez-Enciso M, Ramirez-Gonzalez R, Reecy JM, Zas SR, Rohrer GA, Rund L, Sang YM, Schachtschneider K, Schraiber JG, Schwartz J, Scobie L, Scott C, Searle S, Servin B, Southey BR, Sperber G, Stadler P, Sweedler JV, Tafer H, Thomsen B, Wali R, Wang J, Wang J, White S, Xu X, Yerle M, Zhang GJ, Zhang JG, Zhang J, Zhao SH, Rogers J, Churcher C, Schook LB . Analyses of pig genomes provide insight into porcine demography and evolution. Nature, 2012,491(7424):393-398. doi: 10.1038/nature11622
[2]	Almada AE, Wagers AJ . Molecular circuitry of stem cell fate in skeletal muscle regeneration, ageing and disease. Nat Rev Mol Cell Biol, 2016,17(5):267-279. doi: 10.1038/nrm.2016.7 pmid: 26956195
[3]	Sacco A, Doyonnas R, Kraft P, Vitorovic S, Blau HM . Self-renewal and expansion of single transplanted muscle stem cells. Nature, 2008,456(7221):502-506. doi: 10.1038/nature07384 pmid: 18806774
[4]	Allen RE, Merkel RA, Young RB . Cellular aspects of muscle growth: myogenic cell proliferation. J Anim Sci, 1979,49(1):115-127. doi: 10.2527/jas1979.491115x pmid: 500507
[5]	Buckingham M . Myogenic progenitor cells and skeletal myogenesis in vertebrates. Curr Opin Genet Dev, 2006,16(5):525-532. doi: 10.1016/j.gde.2006.08.008
[6]	Jathar S, Kumar V, Srivastava J, Tripathi V . Technological developments in lncRNA biology. Adv Exp Med Biol, 2017,1008:283-323. doi: 10.1007/978-981-10-5203-3_10 pmid: 28815544
[7]	Yang F, Yi F, Cao HQ, Liang ZC, Du Q . The emerging landscape of long non-coding RNAs. Hereditas(Beijing), 2014,36(5):456-468. doi: 10.3724/SP.J.1005.2014.0456
	杨峰, 易凡, 曹慧青, 梁子才, 杜权 . 长链非编码RNA研究进展. 遗传, 2014,36(5):456-468. doi: 10.3724/SP.J.1005.2014.0456
[8]	Lu C, Huang YH . Progress in long non-coding RNAs in animals. Hereditas(Beijing), 2017,39(11):1054-1065. doi: 10.16288/j.yczz.17-120 pmid: 29254923
	路畅, 黄银花 . 动物长链非编码RNA研究进展. 遗传, 2017,39(11):1054-1065. doi: 10.16288/j.yczz.17-120 pmid: 29254923
[9]	Weikard R, Demasius W, Kuehn C . Mining long noncoding RNA in livestock. Anim Genet, 2017,48(1):3-18. doi: 10.1111/age.12493 pmid: 27615279
[10]	Li H, Feng JC, Li GL, Wang X, Li MZ, Liu HF . The effect of lnc-RAP3 on 3T3-L1 preadipocyte differentiation in mouse. Hereditas(Beijing), 2018,40(9):758-766. doi: 10.16288/j.yczz.18-053 pmid: 30369479
	李欢, 冯晋川, 李贵林, 王讯, 李明洲, 刘海峰 . Lnc- RAP3对小鼠3T3-L1前脂肪细胞分化的影响. 遗传, 2018,40(9):758-766. doi: 10.16288/j.yczz.18-053 pmid: 30369479
[11]	Xie SH . Integrated analysis of miRNA and mRNA expression profiles of muscle development in Changbai and Lantang pigs[Dissertation]. Sun Yat-Sen University, 2017.
	谢水华 . 长白猪和蓝塘猪肌肉发育差异miRNA和mRNA表达谱的整合分析[学位论文]. 中山大学, 2017.
[12]	Tang ZL, Wu Y, Yang YL, Yang YCT, Wang ZS, Yuan JP, Yang Y, Hua CJ, Fan XH, Niu GL, Zhang YB, Lu ZJ, Li K . Comprehensive analysis of long non-coding RNAs highlights their spatio-temporal expression patterns and evolutional conservation in Sus scrofa. Sci Rep, 2017,7:43166. doi: 10.1038/srep43166 pmid: 28233874
[13]	Zhou ZY, Li AM, Adeola AC, Liu YH, Irwin DM, Xie HB, Zhang YP . Genome-Wide identification of long intergenic noncoding RNA genes and their potential association with domestication in pigs. Genome Biol Evol, 2014,6(6):1387-1392. doi: 10.1093/gbe/evu113 pmid: 24891613
[14]	Yun L, Liu JP, Zhuang ZX, Yang LQ, Zhang RL, Ye XM, Cheng JQ . Real-time RT-PCR gene expression relative quantification REST ^© software analysis compared with 2 ^(-ΔΔCT)method . J Trop Med, 2007,7(10):956-958.
	庾蕾, 刘建平, 庄志雄, 杨淋清, 张仁利, 叶小明, 程锦泉 . 实时RT-PCR基因表达相对定量REST ^©软件分析与2 ^(-ΔΔCT)法比较 . 热带医学杂志, 2007,7(10):956-958.
[15]	Chen G, Shi TL, Shi LM . Characterizing and annotating the genome using RNA-seq data. Sci China Life Sci, 2017,60(2):116-125. doi: 10.1007/s11427-015-0349-4 pmid: 27294835
[16]	Djebali S, Davis CA, Merkel A, Dobin A, Lassmann T, Mortazavi A, Tanzer A, Lagarde J, Lin W, Schlesinger F, Xue C, Marinov GK, Khatun J, Williams BA, Zaleski C, Rozowsky J, Röder M, Kokocinski F, Abdelhamid RF, Alioto T, Antoshechkin I, Baer MT, Bar NS, Batut P, Bell K, Bell I, Chakrabortty S, Chen X, Chrast J, Curado J, Derrien T, Drenkow J, Dumais E, Dumais J, Duttagupta R, Falconnet E, Fastuca M, Fejes-Toth K, Ferreira P, Foissac S, Fullwood MJ, Gao H, Gonzalez D, Gordon A, Gunawardena H, Howald C, Jha S, Johnson R, Kapranov P, King B, Kingswood C, Luo OJ, Park E, Persaud K, Preall JB, Ribeca P, Risk B, Robyr D, Sammeth M, Schaffer L, See LH, Shahab A, Skancke J, Suzuki AM, Takahashi H, Tilgner H, Trout D, Walters N, Wang H, Wrobel J, Yu Y, Ruan X, Hayashizaki Y, Harrow J, Gerstein M, Hubbard T, Reymond A, Antonarakis SE, Hannon G, Giddings MC, Ruan Y, Wold B, Carninci P, Guigó R, Gingeras TR . Landscape of transcription in human cells. Nature, 2012,489(7414):101-108. doi: 10.1038/nature11233
[17]	Deniz E, Erman B . Long noncoding RNA (lincRNA), a new paradigm in gene expression control. Funct Integr Genomics, 2017,17(2-3):135-143. doi: 10.1007/s10142-016-0524-x pmid: 27681237
[18]	Jandura A, Krause HM . The new RNA world: growing evidence for long noncoding RNA functionality. Trends Genet, 2017,33(10):665-676. doi: 10.1016/j.tig.2017.08.002 pmid: 28870653
[19]	Li YY, Chen XN, Sun H, Wang HT . Long non-coding RNAs in the regulation of skeletal myogenesis and muscle diseases. Cancer Lett, 2018,417:58-64. doi: 10.1016/j.canlet.2017.12.015 pmid: 29253523
[20]	Flynn RA, Chang HY . Long noncoding RNAs in cell-fate programming and reprogramming. Cell Stem Cell, 2014,14(6):752-761. doi: 10.1016/j.stem.2014.05.014
[21]	Qin CY, Cai H, Qing HR, Li L, Zhang HP . Recent advances on the role of long non-coding RNA H19 in regulating mammalian muscle growth and development. Hereditas(Beijing), 2017,39(12):1150-1157. doi: 10.16288/j.yczz.17-193 pmid: 29258985
	秦辰雨, 蔡禾, 卿涵睿, 李利, 张红平 . 长链非编码RNA H19对哺乳动物肌肉生长发育的调控. 遗传, 2017,39(12):1150-1157. doi: 10.16288/j.yczz.17-193 pmid: 29258985
[22]	Zhou R, Wang YX, Long KR, Jiang AA, Jin L . Regulatory mechanism for lncRNAs in skeletal muscle development and progress on its research in domestic animals. Hereditas(Beijing), 2018,40(4):292-304.
	周瑞, 王以鑫, 龙科任, 蒋岸岸, 金龙 . LncRNA调控骨骼肌发育的分子机制及其在家养动物中的研究进展. 遗传, 2018,40(4):292-304.
[23]	Li MX, Huang T, Ma LP, Liu Y, Li T, Gong HB, Qiu MY, Xie S, Sun XM . Cloning of porcine lncRNA-ENSSSCT00000018610 and its expression pattern in porcine ovarian follicles. Acta Vet Et Zootech Sin, 2018,49(9):1830-1839.
	李梦寻, 黄涛, 马力鹏, 刘乙, 李涛, 公红斌, 邱梅玉, 谢苏, 孙晓梅 . 猪lncRNA-ENSSSCT00000018610的克隆及其在猪卵泡中的表达. 畜牧兽医学报, 2018,49(9):1830-1839.
[24]	Picard B, Lefaucheur L, Berri C, Duclos MJ . Muscle fibre ontogenesis in farm animal species. Reprod Nutr Dev, 2002,42(5):415-431. doi: 10.1051/rnd:2002035 pmid: 12537254
[25]	Lefaucheur L, Vigneron P . Post-natal changes in some histochemical and enzymatic characteristics of three pig muscles. Meat Sci, 1986,16(3):199-216. doi: 10.1016/0309-1740(86)90026-4 pmid: 22054929
[26]	Zammit PS, Heslop L, Hudon V, Rosenblatt JD, Tajbakhsh S, Buckingham ME, Beauchamp JR, Partridge TA . Kinetics of myoblast proliferation show that resident satellite cells are competent to fully regenerate skeletal muscle fibers. Exp Cell Res, 2002,281(1):39-49. doi: 10.1006/excr.2002.5653 pmid: 12441128
[27]	Liao Q, Liu CN, Yuan XY, Kang SL, Miao RY, Xiao H, Zhao GG, Luo HT, Bu DC, Zhao HT, Skogerbø G, Wu ZD, Zhao Y . Large-scale prediction of long non-coding RNA functions in a coding-non-coding gene co-expression network. Nucleic Acids Res, 2011,39(9):3864-3878. doi: 10.1093/nar/gkq1348 pmid: 21247874
[28]	Sanoudou D, Haslett JN, Kho AT, Guo SQ, Gazda HT, Greenberg SA, Lidov HGW, Kohane IS, Kunkel LM, Beggs AH . Expression profiling reveals altered satellite cell numbers and glycolytic enzyme transcription in nemaline myopathy muscle. Proc Natl Acad Sci USA, 2003,100(8):4666-4671. doi: 10.1073/pnas.0330960100 pmid: 12677001
[29]	Mangano KM, Sahni S, Kerstetter JE, Kenny AM, Hannan MT . Polyunsaturated fatty acids and their relation with bone and muscle health in adults. Curr Osteoporos Rep, 2013,11(3):203-212. doi: 10.1007/s11914-013-0149-0 pmid: 23857286
[30]	Lambadiari V, Triantafyllou K, Dimitriadis GD . Insulin action in muscle and adipose tissue in type 2 diabetes: The significance of blood flow. World J Diabetes, 2015,6(4):626-633. doi: 10.4239/wjd.v6.i4.626 pmid: 25987960

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

lncRNA TCONS_00815878对猪骨骼肌卫星细胞分化的影响

The effect of lncRNA TCONS_00815878 on differentiation of porcine skeletal muscle satellite cells

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 30

相关文章 15

编辑推荐

Metrics

目的基因	引物序列( 5′→3′)	复性温度(℃)	产物大小(bp)
TCONS_00815878	F: ACACCCTTTCCCAAAATCAA	56	93
TCONS_00815878	R: GCAGACTGTCCAAATCTACCCT	56	93
PFKM	F: GAGAGCGTTTCCATGATGCTTC	60	221
PFKM	R: AATCAAAGAGGGTGCCATCCAT	60	221
ELOVL5	F: ATATGAAGATCATCCGCGTGCT	60	59
ELOVL5	R: GTGATCTGGTGGTTGTTCTTGC	60	59
MBTPS1	F: CCTCAACAGTGGTGGAATACGA	60	179
MBTPS1	R: TTGGGATGATCTTCAAGCGTCA	60	179
MyoD	F: GGCTGCCCAAGGTGGAAATC	60	170
MyoD	R: TGCGTCTGAGTCACCGCTGTAG	60	170
MyoG	F: ATGAGACATCCCCCTACTTCTACCA	60	160
MyoG	R: GTCCCCAGCCCCTTATCTTCC	60	160
Pax7	F: GGAGTACAAGAGGGAGAACCC	60	122
Pax7	R: TTCTGAGCACGCGGCTAATC	60	122
Myf5	F: GAATGCCATCCGCTACAT	60	125
Myf5	R: AACTGCTGCTCTTTCTGG	60	125
MyHC	F: GTTCAGAGAAAGGCATCCCAAA	60	135
MyHC	R: GAGAGTGACCGACACCACAAGTG	60	135
FKBP10	F: ATGTGTCCTGGAGAGAGAAGGA	60	209
FKBP10	R: AATCAAAGAGGGTGCCATCCAT	60	209
18S rRNA	F: TCCCGACGTGACTGCTC	60	133
18S rRNA	R: GGTGACAGCGGGGTGG	60	133

[1]	吴玲玲, 张小玉, 李晓, 靳建军, 杨公社, 史新娥. miR-196b-5p促进成肌细胞增殖分化[J]. 遗传, 2023, 45(5): 435-446.
[2]	赵欢, 周斌. 胰岛beta细胞再生研究进展[J]. 遗传, 2022, 44(5): 370-382.
[3]	余志鑫, 李鹏宇, 李凯, 缪时英, 王琳芳, 宋伟. 精原干细胞微环境研究进展[J]. 遗传, 2022, 44(12): 1103-1116.
[4]	邹礼平, 潘铖, 王梦馨, 崔林, 韩宝瑜. 激素调控植物成花机理研究进展[J]. 遗传, 2020, 42(8): 739-751.
[5]	杜坤, 毛初阳, 任安勇, 吴雪梅, 李庆玲, 陈婷婷, 陈仕毅, 赖松家. 家兔前体脂肪细胞分化不同时期基因表达谱分析[J]. 遗传, 2020, 42(3): 309-320.
[6]	陈万银, 颜一丹, 栾晓瑾, 王敏, 方杰. CG8005基因在果蝇睾丸生殖细胞中的功能分析[J]. 遗传, 2020, 42(11): 1122-1132.
[7]	杨科, 薛征, 吕湘. 细胞终末分化过程中三维基因组结构与功能调控的分子机制[J]. 遗传, 2020, 42(1): 32-44.
[8]	李欢, 冯晋川, 李贵林, 王讯, 李明洲, 刘海峰. Lnc-RAP3对小鼠3T3-L1前脂肪细胞分化的影响[J]. 遗传, 2018, 40(9): 758-766.
[9]	任岚,肖茹丹,张倩,娄晓敏,张昭军,方向东. KLF1和KLF9对K562细胞红系分化的协同调控作用[J]. 遗传, 2018, 40(11): 998-1006.
[10]	杨熳,卢冰婕,段媛媛,陈晓峰,马建岗,郭燕. 骨质疏松症易感基因BDNF的遗传学关联分析及功能研究[J]. 遗传, 2017, 39(8): 726-736.
[11]	亢逸,关桂君,洪云汉. 用模式生物青鳉概观硬骨鱼性别决定及性分化研究进展[J]. 遗传, 2017, 39(6): 441-454.
[12]	徐妙云, 朱佳旭, 张敏, 王磊. 植物miR169/NF-YA调控模块研究进展[J]. 遗传, 2016, 38(8): 700-706.
[13]	贾振伟. 线粒体与多潜能干细胞功能[J]. 遗传, 2016, 38(7): 603-611.
[14]	吴骏,张俊红,黄蒙慧,朱敏慧,童再康. 光皮桦miR164及其靶基因NAC1在低氮胁迫中的表达分析[J]. 遗传, 2016, 38(2): 155-162.
[15]	周学, 杜宜兰, 金萍, 马飞. 癌症相关microRNA与靶基因的生物信息学分析[J]. 遗传, 2015, 37(9): 855-864.