遗传 ›› 2006, Vol. 28 ›› Issue (11): 1396-1400.doi: 10.1360/yc-006-1396

• 研究报告 • 上一篇    下一篇

含有抗白粉病基因的黑麦染色体小片段向小麦的转移

符书兰1; 唐宗祥1; 张怀琼1; 杨足君2; 任正隆1,2   

  1. 1. 四川农业大学植物遗传和育种省级重点实验室, 雅安 625014; 2. 电子科技大学生命科学与技术学院, 成都 610054

  • 收稿日期:2005-10-31 修回日期:2006-01-06 出版日期:2006-11-05 发布日期:2006-11-05
  • 通讯作者: 任正隆

Transfer of a Rye Small Chromosomal Segment with Powdery Mil-dew- Resistant Gene(s) into Common Wheat (Triticum aestivum L.)

FU Shu-Lan1; TANG Zong-Xiang1; ZHANG Huai-Qiong1; YANG Zu-Jun2; REN Zheng-Long1,2

  

  1. 1. State Key Laboratory for Plant Genetics and Breeding, Sichuan Agricultural University, Ya’an 625014, China;
    2. School of life science and technology, University of Electronic Science and Technology of China, Chengdu 610054 ,China
  • Received:2005-10-31 Revised:2006-01-06 Online:2006-11-05 Published:2006-11-05
  • Contact: REN Zheng-Long

摘要:

利用感白粉病的小麦品种绵阳11的纯系和黑麦自交系R12杂交, 在其单体附加系自交后代的BC1F5株系中选择小麦-黑麦异源易位系。根据已报道的黑麦特异重复序列pSc20H设计了一对特异引物, 用PCR方法鉴定了300个单体附加系的自交BC1F5株系,发现其中70个株系含有黑麦染色体成分。一个来源于6R单体附加系的小麦株系96Ⅱ691-830-98表现了对白粉病的高度抗性, PCR方法鉴定证明其含有黑麦染色体成分。对该株系作进一步的基因组原位杂交(GISH)鉴定, 证明它的一对染色体的端部含有黑麦染色体的小片段。这一结果指出, 含有抗白粉病基因的黑麦染色体6R小片段被引入了小麦。研究表明利用单体附加诱导染色体小片段易位是一种有效的方法。利用PCR和GISH原位杂交相结合的方法可提高检测外源染色体小片段的准确性和选择效率。

关键词: 白粉病, 小片段易位, 小麦, 黑麦, GISH

Abstract:

The advanced progeny lines (BC1F5) from the monosomic addition lines between common wheat cultivar Mianyang 11, which is highly susceptible to powdery mildew, and an inbred rye line R12 were analyzed for selection of wheat-rye translocations. Based on a rye-specific repetitive sequence of pSc20H, which spread over all chromosomes of rye but did not existed in wheat, a set of PCR primer was designed and used to identify the rye chromosome segments in wheat. From 300 of the BC1F5 progeny lines 70 were found to contain chromosome composition of rye. An advanced line, 96Ⅱ691-830-98, originated from 6R monosomic addition line was observed to be immune to powdery mildew, different from its wheat parent Mianyang 11. A small segment of rye chromosome at telomere in a pair of wheat chromosome in the line was found by means of GISH. The results indicated that a small segment of rye chromosome 6R carrying the gene(s) for resistance to powdery mildew has been transferred into common wheat. In the progeny of monosomic addition lines a high frequency of wheat-alien species translocation with various segments of chromosomes could be found by application of both PCR and GISH technique.

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