遗传 ›› 2007, Vol. 29 ›› Issue (3): 259-259―264.doi: 10.1360/yc-007-0259

• 专论与综述 • 上一篇    下一篇

标记辅助导入的原理与应用

白俊艳1, 张勤2, 贾小平3   

  1. 1. 河南科技大学动物科技学院, 洛阳 471003;
    2. 中国农业大学动物科学技术学院, 农业部畜禽遗传育种重点实验室, 北京 100094;
    3. 中国农业大学生物学院, 北京 100094

  • 收稿日期:2006-07-02 修回日期:2006-10-24 出版日期:2007-03-01 发布日期:2007-03-01
  • 通讯作者: 张勤

Theory and application of marker-assisted introgression

BAI Jun-Yan1, ZHANG Qin2, JIA Xiao-Ping3

  

  1. 1. Animal Science and Technology, Henan Science and Technology University, Luoyang 471003, China; 2. Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Chinese Agriculture University, Beijing 100094, China; 3. College of Biological Science, China Agricultural University, Beijing 100094, China
  • Received:2006-07-02 Revised:2006-10-24 Online:2007-03-01 Published:2007-03-01
  • Contact: ZHANG Qin

摘要:

标记辅助导入是分子遗传信息应用于动物育种的一个重要方面, 其目的是在标记信息的辅助下将一个品种(供体)中的一个或多个优良基因导入另一个品种(受体), 同时还要尽可能地保持受体群体原有的遗传背景。在标记辅助导入过程中, 标记信息既可用于辅助前景选择, 即对目标基因携带者的选择, 以保证目标基因的正确导入, 也可用于辅助背景选择, 即对受体基因组的选择, 以加快受体遗传背景的恢复。本文介绍了标记辅助导入的原理和基本方法, 综述了目前已提出的不同前景选择和背景选择方法以及消除遗传累赘(与目标基因连锁的不利基因)的方法, 同时列举了标记辅助导入在动物上的一些成功应用。

关键词: 标记辅助导入, 前景选择, 动物, 背景选择

Abstract:

Marker-assisted introgression (MAI) is one of the major applications of molecular information in animal breeding aiming at introgression of one or more favorable genes from a line (donor) to another (recipient), while keeping the genetic background of the recipient as much as possible. It consists of three phases. Firstly, cross donor and recipient lines to produce F1; secondly, repeated backcross the F1 individuals and the subsequent progenies with the recipient to recover its genetic background; and thirdly, intercross the backcrossed progenies to fix the introgressed genes in the population. Many factors affect the efficiency of MAI, among which the methods of foreground and background selection are very critical.