遗传 ›› 2007, Vol. 29 ›› Issue (10): 1256-1256―1262.doi: 10.1360/yc-007-1256

• 研究报告 • 上一篇    下一篇

利用基于PCR的分子标记区分普通小麦-提莫菲维小麦渐渗系

纪剑辉; 曹爱忠; 王海燕; 覃碧; 王苏玲; 孔芳; 陈佩度; 刘大钧; 王秀娥   

  1. 南京农业大学作物遗传与种质创新国家重点实验室细胞遗传所, 南京 210095

  • 收稿日期:2007-01-29 修回日期:2007-04-05 出版日期:2007-10-10 发布日期:2007-10-10
  • 通讯作者: 王秀娥

Discrimination of the Triticum aestivum-T. timopheevii introgression lines using PCR-based molecular markers

JI Jian-Hui; CAO Ai-Zhong; WANG Hai-Yan; QIN Bi; WANG Su-Ling; KONG Fang; CHEN Pei-Du; LIU Da-Jun; WANG Xiu-E

  

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China

  • Received:2007-01-29 Revised:2007-04-05 Online:2007-10-10 Published:2007-10-10
  • Contact: wang xiu-e

摘要:

采用定位于小麦2B染色体上的72对分子标记对含小麦抗白粉病基因Pm6的8份普通小麦(T.aestivum L.)-提莫菲维(T.timopheevii zhuk.)渐渗系材料进行分析, 通过分子标记标图确定8份材料中渗入的提莫菲维小麦染色体片段的大小, 同时结合连锁图谱对这些材料进行了遗传和物理标图。参考本研究所用的分子标记在染色体2B上的定位结果, Pm6基因被位于2B 染色体长臂近末端2BL-6区域, 提莫菲维小麦2G染色体渐渗片断长度由短到长排列顺序为: IGV1-465Pm6基因的克隆奠定了基础。

关键词: Pm6, 白粉病, 渐渗系, 提莫菲维小麦, 小麦

Abstract:

In order to determine the fragment size of Triticum timopheevii chromosome segments introduced into wheat background and physically map the Pm6 gene, a total of 72 primers (including SSR and STS primers) were used to analyze the eight introduced introgression lines containing Pm6 gene. Referring to the available mapping information of the analyzed markers on chromosome 2B, Pm6 was physically located in distal part of the long arm of chromosome 2B at the region of Bin 2BL-6. The introgressed fragment sizes of the chromosome 2G in different introgression lines was determined to be as follow (from short to long): IGV1-465Pm6 gene.