遗传 ›› 2017, Vol. 39 ›› Issue (2): 127-134.doi: 10.16288/j.yczz.16-379

• 综述 • 上一篇    下一篇

高通量测序时代下持家基因定义的发展

魏凯(),马磊()   

  1. 石河子大学生命科学学院,石河子 832000
  • 收稿日期:2016-11-11 修回日期:2017-01-24 出版日期:2017-02-20 发布日期:2017-02-10
  • 作者简介:魏凯,硕士研究生,专业方向:遗传学。E-mail: weikai320722@163.com|马磊,博士,副教授,研究方向:生物信息学与分子遗传学。E-mail: malei1979@hotmail.com
  • 基金资助:
    国家自然科学基金项目(31560310,31272416);石河子大学青年教师与名师结对子培养计划项目(SDJDZ201504)

Concept development of housekeeping genes in the high-throughput sequencing era

Wei Kai(),Ma Lei()   

  1. College of Life Science, Shihezi University, Shihezi 832000, China
  • Received:2016-11-11 Revised:2017-01-24 Online:2017-02-20 Published:2017-02-10
  • Supported by:
    the National Natural Science Foundation of China(31560310,31272416);the Scholar Pair-training Program of Shihezi University(SDJDZ201504)

摘要:

持家基因一般指在所有细胞中均稳定表达的一类组成型基因,其产物是维持细胞基本生命活动所必需的。持家基因的定义及其发展,反映了其本质和时代特征。本文从基因表达水平量化技术的角度,结合作者利用转录组数据对猪持家基因的分析,讨论了持家基因的定义及其发展,尤其是技术的进步及数据量的剧增对持家基因的定义所引发的问题。本文总结发现,应该在研究技术的进步中,动态地认识持家基因定义所具有的层次性,从而对持家基因的定义不断赋予新的时代特征。

关键词: 持家基因, 转录组测序, 基因表达

Abstract:

Housekeeping genes are typically constitutive genes that are required for the maintenance of basic cellular functions, and are stably expressed in all cells of an organism under all conditions and during all developmental stages. The definition and development of housekeeping genes reflect their essence and characteristics at different times. The present study reviews the concept development of housekeeping genes based on the technologies that quantify gene expression levels. We also analyze pig housekeeping genes by RNA-seq data, and discuss conceptual issues raised by the rapid progress of technologies and the growth of data. Our present study summarizes that housekeeping genes should be defined at different levels, and changing times will witness its conceptual development.

Key words: housekeeping genes, transcriptome sequencing, gene expression