水稻qGL3.4调控籽粒大小与株型

doi:10.16288/j.yczz.23-064

遗传 ›› 2023, Vol. 45 ›› Issue (9): 835-844.doi: 10.16288/j.yczz.23-064

水稻qGL3.4调控籽粒大小与株型

郑镇武^1,²(), 赵宏源^1,², 梁晓娅^1,², 王一珺^1,², 王驰航^1,², 巩高洋^1,², 黄金燕^1,², 张桂权^1,², 王少奎^1,²(), 刘祖培^1,²()

1.华南农业大学，广东省植物分子育种重点实验室，亚热带农业生物资源保护与利用国家重点实验室，广州 510642
2.广东省岭南现代农业重点实验室，广州 510630

收稿日期:2023-03-20 修回日期:2023-06-27 出版日期:2023-09-20 发布日期:2023-07-28
通讯作者: 王少奎,刘祖培 E-mail:958779674@qq.com;lzp2020@scau.edu.cn;shaokuiwang@scau.edu.cn
作者简介:郑镇武，硕士研究生，专业方向：作物遗传育种。E-mail: 958779674@qq.com
基金资助:
广东省自然科学基金面上项目(2023A1515012083);广州市科技计划项目(2023A04J1962);国家自然科学青年基金项目(32201742);双一流学科建设项目(2021B10564001)

qGL3.4 controls kernel size and plant architecture in rice

Zhenwu Zheng^1,²(), Hongyuan Zhao^1,², Xiaoya Liang^1,², Yijun Wang^1,², Chihang Wang^1,², Gaoyan Gong^1,², Jinyan Huang^1,², Guiquan Zhang^1,², Shaokui Wang^1,²(), Zupei Liu^1,²()

1. Guangdong Provincial Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China
2. Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510630, China

Received:2023-03-20 Revised:2023-06-27 Online:2023-09-20 Published:2023-07-28
Contact: Shaokui Wang,Zupei Liu E-mail:958779674@qq.com;lzp2020@scau.edu.cn;shaokuiwang@scau.edu.cn
Supported by:
Natural Science Foundation of Guangdong Province(2023A1515012083);Science and Technology Planning Project of Guangzhou(2023A04J1962);National Natural Science Foundation of China(32201742);Double First-class Discipline Promotion Project(2021B10564001)

摘要/Abstract

摘要：

籽粒大小与株型对水稻产量具有重要影响，因此其相关基因克隆与功能研究对培育高产水稻具有重大的意义。本研究从以短舌野生稻为供体、华粳籼74 (HJX74)为受体的染色体单片段代换系(SSSLs)中鉴定到一个新的调控籽粒大小与株型的QTL位点qGL3.4。与对照HJX74相比，近等基因系NIL-qGL3.4的粒长、粒宽、千粒重、穗长、穗粒数、一次枝梗数、单株产量与株高显著增加，而NIL-qGL3.4的分蘖数和二级枝梗数与HJX74对应值无显著差异。通过图位克隆，将qGL3.4定位在第3号染色体239.18 kb区间内。细胞学分析表明，NIL-qGL3.4通过调节颖壳细胞的生长进而调控籽粒的大小。分子机理研究表明，qGL3.4可能通过调控籽粒大小相关基因EXPANSINs、GS3、GL3.1、PGL1、GL7、OsSPL13和GS5的表达进而调控籽粒大小。本研究可能为高产与理想株型的水稻培育提供新的靶标位点。

关键词: 水稻, 籽粒大小, 株型, 单片段代换系

Abstract:

Kernel size and plant architecture play important roles in kernel yield in rice. Cloning and functional study of genes related to kernel size and plant architecture are of great significance for breeding high-yield rice. Using the single-segment substitution lines which developed with Oryza barthii as a donor parent and an elite indica cultivar Huajingxian74 (HJX74) as a recipient parent, we identified a novel QTL (quantitative trait locus), named qGL3.4, which controls kernel size and plant architecture. Compared with HJX74, the kernel length, kernel width, 1000-kernel weight, panicle length, kernels per plant, primary branches, yield per plant, and plant height of near isogenic line-qGL3.4 (NIL-qGL3.4) are increased, whereas the panicles per plant and secondary branches per panicle of NIL-qGL3.4 are comparable to those of HJX74. qGL3.4 was narrowed to a 239.18 kb interval on chromosome 3. Cell analysis showed that NIL-qGL3.4 controlled kernel size by regulating cell growth. qGL3.4 controls kernel size at least in part through regulating the transcription levels of EXPANSINS, GS3, GL3.1, PGL1, GL7, OsSPL13 and GS5. These results indicate that qGL3.4 might be beneficial for improving kernel yield and plant architecture in rice breeding.

Key words: rice, kernel size, plant architecture, single segment substitution line

郑镇武, 赵宏源, 梁晓娅, 王一珺, 王驰航, 巩高洋, 黄金燕, 张桂权, 王少奎, 刘祖培. 水稻qGL3.4调控籽粒大小与株型[J]. 遗传, 2023, 45(9): 835-844.

Zhenwu Zheng, Hongyuan Zhao, Xiaoya Liang, Yijun Wang, Chihang Wang, Gaoyan Gong, Jinyan Huang, Guiquan Zhang, Shaokui Wang, Zupei Liu. qGL3.4 controls kernel size and plant architecture in rice[J]. Hereditas(Beijing), 2023, 45(9): 835-844.

图/表 11

图1

图2

图3

图4

附图1

附图2

附图3

附图4

附表1

定位区间内注释基因信息"

登录号	是否克隆	功能
LOC_Os03g11790	未克隆	OsFBX79，含有F-box结构域的蛋白
LOC_Os03g11810	未克隆	表达蛋白
LOC_Os03g11819	未克隆	表达蛋白
LOC_Os03g11830	未克隆	转座子蛋白，假定
LOC_Os03g11840	未克隆	表达蛋白
LOC_Os03g11850	未克隆	逆转录转座子，Ty1-copia亚类，假定
LOC_Os03g11860	未克隆	表达蛋白
LOC_Os03g11874	未克隆	表达蛋白
LOC_Os03g11890	未克隆	马铃薯Y病毒VPg相互作用蛋白，假定
LOC_Os03g11900	已克隆	单糖转运蛋白，调节一系列单糖的跨膜运输
LOC_Os03g11910	未克隆	Dnak家族蛋白，假定
LOC_Os03g11930	未克隆	表达蛋白
LOC_Os03g11950	未克隆	SEC14胞质因子家族蛋白，假定
LOC_Os03g11960	未克隆	铜/锌超氧化物歧化酶，假定
LOC_Os03g11970	未克隆	含有微管蛋白/FtsZ结构域的蛋白质，假定
LOC_Os03g11980	未克隆	β-氨基己糖苷酶前体，假定
LOC_Os03g11990	未克隆	bolA，假定
LOC_Os03g12000	未克隆	DEAD-box ATP-dependent RNA解旋酶，假定
LOC_Os03g12010	未克隆	表达蛋白
LOC_Os03g12020	未克隆	50S核糖体蛋白L15，叶绿体前体，假定
LOC_Os03g12030	已克隆	编码脂肪酸延长酶，影响结实率和叶片角质层蜡的含量
LOC_Os03g12040	未克隆	表达蛋白
LOC_Os03g12050	未克隆	表达蛋白
LOC_Os03g12064	未克隆	表达蛋白
LOC_Os03g12080	未克隆	逆转录转座子蛋白，假定
LOC_Os03g12100	未克隆	逆转录转座子，假定
LOC_Os03g12110	未克隆	高半胱氨酸S-甲基转移酶蛋白，假定
LOC_Os03g12120	未克隆	无顶端分生组织蛋白，假定
LOC_Os03g12140	未克隆	葡聚糖内切-1，3-β-葡萄糖苷酶前体，假定
LOC_Os03g12150	未克隆	丝氨酸/苏氨酸蛋白激酶受体前体，假定
LOC_Os03g12160	未克隆	富含亮氨酸重复家族蛋白，假定
LOC_Os03g12170	未克隆	表达蛋白
LOC_Os03g12190	未克隆	OsFBX80，含有F-box结构域的蛋白
LOC_Os03g12200	未克隆	OsFBX81，含有F-box结构域的蛋白
LOC_Os03g12210	未克隆	表达蛋白

附表1

附表2

附表3

本研究所用引物列表"

引物名称	序列	目的
RM545F	CAATGGCAGAGACCCAAAAG	定位
RM545R	CTGGCATGTAACGACAGTGG	定位
OSR16F	AAAACTAGCTTGCAAAGGGGA	定位
OSR16R	TGCCGGCTGATCTTGTTCTC	定位
RM7576F	CTGCCCTGCCTTTTGTACAC	定位
RM7576R	GCGAGCATTCTTTCTTCCAC	定位
RM517F	GGCTTACTGGCTTCGATTTG	定位
RM517R	CGTCTCCTTTGGTTAGTGCC	定位
RM6291F	CGCTGGAACGAGACGAAC	定位
RM6291R	TGGGTTGGGCTCTACAAAAC	定位
PSM428F	GATAGAGCAGGGCTGGAACA	定位
PSM428R	ATGCTTGCCTTAGTGTCCG	定位
RM3766F	TTATAGAGCCAACACAACGG	定位
RM3766R	ATCGATCTCTCTCCTGGAAA	定位
RM3545F	TATCGGCATCAGGGTTCTTC	定位
RM3545R	ATAGCCGTCTTCACCGACAC	定位
RM5639F	GGAAGAACAGAGTTGCTCGG	定位
RM5639R	GTGCCATTTATTTCCGTCCC	定位
RM1338F	GAAGGGATTCAAGGGGTAGG	定位
RM1338R	TCCAGTCATCCCTGTGAAAG	定位
RM5944F	GAGCCGCATCAACCAGTTAC	定位
RM5944R	CAGTACAGCGCGCACTACAC	定位
RM282F	CTGTGTCGAAAGGCTGCAC	定位
RM282R	CAGTCCTGTGTTGCAGCAAG	定位
ZID16-F	AAAATGGAAGCAGGGAAAG	定位
ZID16-R	CTGCCACTACACGGGAGA	定位
ZID3-F	AGCAAGCCAATGGAGGGT	定位
ZID3-R	AACTGAACAAGACAGGGA	定位
ZID10-F	TTGTGCGCTGACCACAAT	定位
ZID10-R	GCCCAACCAATGCAAGTC	定位
ZID14-F	TCTTGTCACAGTGATCCACA	定位
ZID14-R	TTCCTTTAGCTAGTGAAGTTGT	定位
ZID-39F	AAGAGCCCTCGATGTTGA	定位
ZID-39R	GGTGGCAATGCAATGTTT	定位
PA5-F	ACTTCTGCCCGCCGAACAAC	RT-qPCR分析
PA5-R	GCCTTGAAGAGAGCGATGCG	RT-qPCR分析
EXPA13-F	CCTGGGAGAAGATCGGCATC	RT-qPCR分析
EXPA13-R	GAAGTAGTCGTGCCCGTTG	RT-qPCR分析
EXPA24-F	TCTACCGTGCTGGCATCATC	RT-qPCR分析
EXLA4-F	TAAGGTGCAGAGACCGGAG	RT-qPCR分析
EXLA4-R	TCCTCGGTGATGCGAAACTG	RT-qPCR分析
EXPB3-F	AAGATGCGTCAACCACCCTG	RT-qPCR分析
EXPB3-R	AGTGGTACTTGGAAACGGGG	RT-qPCR分析
EXPB6-F	GCCTCGACTCCAACCAC	RT-qPCR分析
EXPB6-R	CTAGCTGAACTGGACGATGG	RT-qPCR分析
EXPB7-F	TTCAAGGACGGCAAGGGATG	RT-qPCR分析
EXPB7-R	GGTACAGCGACACTGGGTAG	RT-qPCR分析
EXPB14-F	AGCTCCCAGTCCAATACGC	RT-qPCR分析
EXPB14-R	CGAGGTAGTACTGGTTGGAGC	RT-qPCR分析
qGS3F	CATCGGAGAAGCGAAGTCAT	RT-qPCR分析
qGS3R	TTGAGGTTGAAGGAGGAGGA	RT-qPCR分析
qGL7F	CCCCTAGCATCGACACCAAG	RT-qPCR分析
qGL7R	CGGGTTCCAGCACTCCTCT	RT-qPCR分析
qGS5F	AGTGGACTGCTTCCAGGGAAG	RT-qPCR分析
qGS5R	CACGCAGTACCGAGAACTGA	RT-qPCR分析
qPGL1F	GCGTCATGAACTTCACCTTCTTCTC	RT-qPCR分析
qPGL1R	ATCGACATCATCTGCACCTGCA	RT-qPCR分析
qGL3.1F	TCACAACTCCCAGGATAGG	RT-qPCR分析
qGL3.1R	TTTGTCTCGCTCGCTCAT	RT-qPCR分析
qOsSPL13F	AACCCGCCGTTCCAGATCAG	RT-qPCR分析
qOsSPL13R	AAGAAGGGACGTAGGTGGTG	RT-qPCR分析
qActin-F	GCTATGTACGTCGCCATCCAG	RT-qPCR分析
qActin-R	AATGAGTAACCACGCTCCGTCA	RT-qPCR分析
qLOC_Os03g11790-F	GCTTCTCCTTTGTTGGAGAC	RT-qPCR分析
qLOC_Os03g11790-R	AAGGCACTCAAGCGAAGTTG	RT-qPCR分析
qLOC_Os03g11810-F	GAGGGAAGCACGGCGATGGG	RT-qPCR分析
qLOC_Os03g11810-R	GTCCTCAGATATCGCTTTGC	RT-qPCR分析
qLOC_Os03g11819-F	GAGCCGGAAGAAATTTTGTC	RT-qPCR分析
qLOC_Os03g11819-R	AAACAACCGGAGACAAGATG	RT-qPCR分析
qLOC_Os03g11830-F	CCGTGACCGAGACTACTTCG	RT-qPCR分析
qLOC_Os03g11830-R	CTGCACCGTGCAAGTCGTCG	RT-qPCR分析
qLOC_Os03g11840-F	CACTCACTTCCGGAGGCAG	RT-qPCR分析
qLOC_Os03g11840-R	CACTGAGGCGACGCCTGATG	RT-qPCR分析
qLOC_Os03g11850-F	TGTACTAGAAGGTCAGGAGG	RT-qPCR分析
qLOC_Os03g11850-R	GCTAGCCAAGTACATCAATG	RT-qPCR分析
qLOC_Os03g11860-F	GATTTCATACATGGAAACG	RT-qPCR分析
qLOC_Os03g11860-R	TGGACTGCCGGGCTGCACGG	RT-qPCR分析
qLOC_Os03g11874-F	CCATATGTCTAAGGCAAATC	RT-qPCR分析
qLOC_Os03g11874-R	CAGACATCGATGTTGCCGTC	RT-qPCR分析
qLOC_Os03g11890-F	GGAGGAAGCGATGGCATTAG	RT-qPCR分析
qLOC_Os03g11890-R	CATTGCTTCCTTTACAGTGC	RT-qPCR分析
qLOC_Os03g11900-F	CCAGCTCAACGTCACCATCG	RT-qPCR分析
qLOC_Os03g11900-R	AGCAGCACGGCGATCACCAG	RT-qPCR分析
qLOC_Os03g11910-F	CTGACCCTTCAGGGAGCCTG	RT-qPCR分析
qLOC_Os03g11910-R	TCGACGGTGCCCTTTGCTGC	RT-qPCR分析
qLOC_Os03g11930-F	GGATTCTCTGCCGGTGAAAG	RT-qPCR分析
qLOC_Os03g11930-R	ACTGTCGCTGCCGTCACCAG	RT-qPCR分析
qLOC_Os03g11950-F	GATCGTCGCCGACTACTACC	RT-qPCR分析
qLOC_Os03g11950-R	GGCTTGAGGGTGTTGTCGTG	RT-qPCR分析
qLOC_Os03g11960-F	CCTCACAATAAGTCCCATGG	RT-qPCR分析
qLOC_Os03g11960-R	AGATCGAAGTCCAATGATAC	RT-qPCR分析
qLOC_Os03g11970-F	TCGAGCCCGCCAGCGTCATG	RT-qPCR分析
qLOC_Os03g11970-R	ACGCGCGCTTGGCGTACATG	RT-qPCR分析
qLOC_Os03g11980-F	CATCGTCCTCCCTACCGTCC	RT-qPCR分析
qLOC_Os03g11980-R	GCACCACCGGGTCGTCCTCC	RT-qPCR分析
qLOC_Os03g11990-F	GGCACTAGTGCTATGCTTTC	RT-qPCR分析
qLOC_Os03g11990-R	CATGAAGTCCTGAGTTCAAC	RT-qPCR分析
qLOC_Os03g12000-F	ATACGAGCAGCCAGGAGTTG	RT-qPCR分析
qLOC_Os03g12000-R	CTCAGTAGCTTTCATCCCAC	RT-qPCR分析
qLOC_Os03g12010-F	GGAATCCAATAGTGGCAGTG	RT-qPCR分析
qLOC_Os03g12010-R	GCTTGGCCAGCTTGATCCTC	RT-qPCR分析
qLOC_Os03g12020-F	TGGTGACGAGATATCATTGG	RT-qPCR分析
qLOC_Os03g12020-R	CTCGAGCTTCTCCTTGGCTG	RT-qPCR分析
qLOC_Os03g12030-F	CAAGCTCCGCAGCAACATCC	RT-qPCR分析
qLOC_Os03g12030-R	TGCGGACCACGTGCATCAGC	RT-qPCR分析
qLOC_Os03g12040-F	GCTGCCGTGACCACCACCTC	RT-qPCR分析
qLOC_Os03g12040-R	CATTAATGCATGTCAGTGG	RT-qPCR分析
qLOC_Os03g12050-F	CGCACATAATTTCGGAATCG	RT-qPCR分析
qLOC_Os03g12050-R	CACATCAAGGCAGGACCTTC	RT-qPCR分析
qLOC_Os03g12064-F	CCCATGTTTGTTGAAGAAAC	RT-qPCR分析
qLOC_Os03g12064-R	GTCCGATTCCTCGTAGGATG	RT-qPCR分析
qLOC_Os03g12080-F	TCCTGAGCGGCAGGGGTTAG	RT-qPCR分析
qLOC_Os03g12080-R	TAGAATCGAGGCGCTCCTCC	RT-qPCR分析
qLOC_Os03g12100-F	AGTCCTCTTTCCAACGGATC	RT-qPCR分析
qLOC_Os03g12100-R	TCCGTGGGTTGTTGATCTTGC	RT-qPCR分析
qLOC_Os03g12110-F	CAGGAGAGAGCTTTGCAGAG	RT-qPCR分析
qLOC_Os03g12110-R	ACAGGTCGTGCTCACCAGCC	RT-qPCR分析
qLOC_Os03g12120-F	TCTTGAGCGACCGGAATATG	RT-qPCR分析
qLOC_Os03g12120-R	AATACCCATTCATCAGGAGC	RT-qPCR分析
qLOC_Os03g12140-F	CGGACAACGTCGAGGCGCAC	RT-qPCR分析
qLOC_Os03g12140-R	ACGTCCCCGATCGCCCGGTG	RT-qPCR分析
qLOC_Os03g12150-F	GACCCTGACTTGGAGCCAAC	RT-qPCR分析
qLOC_Os03g12150-R	TTGACCACGGCATGAACCGG	RT-qPCR分析
qLOC_Os03g12160-F	GGATCCTTTGTTATTAGATC	RT-qPCR分析
qLOC_Os03g12160-R	TCTTCAGAGAACCACTTGAG	RT-qPCR分析
qLOC_Os03g12170-F	TCGTTGCGGTTGTACCTCTAC	RT-qPCR分析
qLOC_Os03g12170-R	GAGGTGGAGGCCTGTGGCTC	RT-qPCR分析
qLOC_Os03g12190-F	GACCCAAATACATTGGCGAGC	RT-qPCR分析
qLOC_Os03g12190-R	GTTGGCTACGGTTTGGATCGTCG	RT-qPCR分析
qLOC_Os03g12200-F	TGGCGAATCATCCGAAGTTAACG	RT-qPCR分析
qLOC_Os03g12200-R	CTGCCATAATATTTTTCAACGAG	RT-qPCR分析
qLOC_Os03g12210-F	TTGCATCCACGCGAGATACG	RT-qPCR分析
qLOC_Os03g12210-R	CCAGGTTGTCCTCGATCTCC	RT-qPCR分析

附表3

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季度	材料名称	粒长	粒宽	千粒重	株高	分蘖数	穗长	单株产量
2021晚季	HJX74	8.285±0.153	2.642±0.111	21.053±0.222	103.9±4.3	7.8±0.91	21.46±1.67	22.72±1.37
2021晚季	NIL-qGL3.4	8.649±0.224**	2.768±0.117*	23.837±0.318**	107.7±3.9**	8.2±1.02	23.47±1.19**	24.68±1.24**
2022早季	HJX74	8.326±0.176	2.673±0.134	22.993±0.492	102.5±5.2	6.8±1.13	21.64±1.16	23.62±2.18
2022早季	NIL-qGL3.4	8.721±0.159**	2.804±0.138**	25.673±0.632**	110.2±6.8**	7.4±1.02	24.63±2.27**	25.73±1.69**
2022晚季	HJX74	8.264±0.253	2.666±0.113	22.872±0.142	100.7±4.9	7.5±1.34	22.11±1.58	22.57±1.44
2022晚季	NIL-qGL3.4	8.678±0.231**	2.768±0.142*	25.028±0.337**	108.2±3.8**	7.4±1.22	23.89±1.26**	24.92±2.01**

水稻qGL3.4调控籽粒大小与株型

qGL3.4 controls kernel size and plant architecture in rice

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