着丝粒蛋白Fta2磷酸化对减数分裂的影响

doi:10.16288/j.yczz.24-038

遗传 ›› 2024, Vol. 46 ›› Issue (7): 552-559.doi: 10.16288/j.yczz.24-038

着丝粒蛋白Fta2磷酸化对减数分裂的影响

倪子涵¹^,²(), 闵羽¹^,², 马玲玲¹^,², 渡边嘉典¹^,²()

1.江南大学未来食品科学中心，无锡 214122
2.江南大学生物工程学院，无锡 214122

收稿日期:2024-03-14 修回日期:2024-05-06 出版日期:2024-07-20 发布日期:2024-05-22
通讯作者: 渡边嘉典，教授，研究方向：染色体分离机制。E-mail: ywatanabe@jiangnan.edu.cn
作者简介:倪子涵，硕士研究生，专业方向：染色体分离机制。E-mail: 641752174@qq.com

The effect of centromere protein Fta2 phosphorylation during meiosis

Zihan Ni¹^,²(), Yu Min¹^,², Lingling Ma¹^,², Yoshinori Watanabe¹^,²()

1. Science Center for Future Foods, Jiangnan University, Wuxi 214122, China
2. Biotechnology School, Jiangnan University,Wuxi 214122,China

Received:2024-03-14 Revised:2024-05-06 Published:2024-07-20 Online:2024-05-22

摘要/Abstract

摘要：

在减数分裂过程中，黏连蛋白(cohesin)在着丝粒区域定位出现缺陷时会导致一系列疾病的产生。黏连蛋白的正确定位离不开装载复合体Mis4-Ssl3的参与，现已知黏连蛋白在装载复合体的帮助下完成装载过程，但是其如何在着丝粒区域定位仍不清楚。基于已有研究报道黏连蛋白在着丝粒的定位由着丝粒蛋白的磷酸化介导，本研究从Sim4着丝粒蛋白复合体组分Fta2蛋白着手，通过生物信息学手段寻找潜在的磷酸化位点，在裂殖酵母(Schizosaccharomyces pombe)中构建了fta2-9A和fta2-9D突变体，并通过噻苯咪唑(thiabendazole，TBZ)敏感度测试和荧光显微定位对其表型进行检测。结果显示，Fta2蛋白的磷酸化对有丝分裂没有影响，但对减数分裂染色体分离存在影响。本研究表明Fta2的磷酸化对减数分裂非常重要，很可能与减数分裂特有的黏连蛋白定位有关。

关键词: 减数分裂, 黏连蛋白, Fta2, 磷酸化, 装载复合体

Abstract:

During meiosis, defects in cohesin localization within the centromere region can result in various diseases. Accurate cohesin localization depends on the Mis4-Ssl3 loading complex. Although it is known that cohesin completes the loading process with the help of the loading complex, the mechanisms underlying its localization in the centromere region remain unclear. Previous studies suggest cohesin localization in the centromere is mediated by phosphorylation of centromeric proteins. In this study, we focused on the Fta2 protein, a component of the Sim4 centromere protein complex. Using bioinformatics methods, potential phosphorylation sites were identified, and fta2-9A and fta2-9D mutants were constructed in Schizosaccharomyces pombe. The phenotypes of these mutants were characterized through testing thiabendazole (TBZ) sensitivity and fluorescent microscopy localization. Results indicated that Fta2 phosphorylation did not impact mitosis but affected chromosome segregation during meiosis. This study suggests that Fta2 phosphorylation is vital for meiosis and may be related to the specific localization of cohesin during this process.

Key words: meiosis, cohesin, Fta2, phosphorylation, loading complex

倪子涵, 闵羽, 马玲玲, 渡边嘉典. 着丝粒蛋白Fta2磷酸化对减数分裂的影响[J]. 遗传, 2024, 46(7): 552-559.

Zihan Ni, Yu Min, Lingling Ma, Yoshinori Watanabe. The effect of centromere protein Fta2 phosphorylation during meiosis[J]. Hereditas(Beijing), 2024, 46(7): 552-559.

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菌株名称	基因型
Y17	h⁺
Y355	h^- leu1 imr1L-GFP mes1-B44
Y491	h⁺ mes1^-B44
N1	h^- leu1 imr1L-GFP mes1-B44 fta2-nat
N2	h⁺ mes1-B44 Fta2-nat
N3	h^- leu1 imr1L-GFP mes1-B44 rec8-2A-kan rec12::hyg
N4	h⁺ mes1-B44 rec8-2A-kan rec12::hyg
N5	h^- leu1 imr1L-GFP mes1-B44 rec8-2A-kan rec12::hyg fta2-nat
N6	h^- leu1 imr1L-GFP mes1-B44 rec8-2A-kan rec12::hyg fta2-nat-9A
N7	h^- leu1 imr1L-GFP mes1-B44 rec8-2A-kan rec12::hyg fta2-nat-9D
N8	h⁺ mes1-B44 rec8-2A-kan rec12::hyg fta2-nat
N9	h⁺ mes1-B44 rec8-2A-kan rec12::hyg fta2-nat-9A
N10	h⁺ mes1-B44 rec8-2A-kan rec12::hyg fta2-nat-9D
N11	h⁺ fta2-nat
N12	h⁺ fta2-nat-9A
N13	h⁺ fta2-nat-9D
N53	h⁺ swi6::kanr

CCAN	S. pombe	S. cerevisiae
C	Cnp3	Mif2
O	Mal2	Mcm21
P	Fta2	Ctf19
Q	Fta7	Okp1
U	Mis17	Ame1
H	Fta3	Mcm16
I	Mis6	Ctf3
K	Sim4	Mcm22
T	Cnp20	Cnn1
W	SPAC17G8.15 (new1)	Wip1
S	SPBC2D10.16	Mhf1
X	SPCC576.12c	Mhf2
N	Mis15	Chl4
L	Fta1	Iml3

着丝粒蛋白Fta2磷酸化对减数分裂的影响

The effect of centromere protein Fta2 phosphorylation during meiosis

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