关键词: 猪, 胰岛素样生长因子2, 遗传效应, 错配PCR-RFLP

Abstract:

A mispairing PCR-RFLP technique was applied in this study to determine the Insulin-like Growth Factor 2(IGF2) gene intron3 G3072A mutation in an outbred Landrace and Large White, and the gelded boars from Landrace×Large White cross. The difference of corresponding traits and the genetic effects of the boars inherited from parental A allele and inherited from parental G allele were analyzed. The results indicated that comparing with the boars inherited from parental G allele, the boars inherited from parental A allele increased significantly in the circumference 3.06% (P<0.05)and index of body 3.01% (P<0.05), respectively. The boars inherited from parental A allele had a significantly less average buttock fat thickness (15.31%, P<0.01) ,thorax-waist fat thickness (23.74%, P<0.01), skin thickness9.38% (P<0.01), fiber density (20.03%, P<0.01) and had more less 6th～7th rib fat thickness (20.27%, P<0.05), tendernce (17.32%, P<0.05), and had more thick shoulder fat thickness (7.97%, P<0.05), and had bigger the loin eye area (22.58%, P<0.01) and fiber cross-sectional area (32.70%, P<0.01) and fiber diameter (15.38%, P<0.01) and lean meat (2.18%, P<0.01)than the boars inherited from parental G allele. The results were suggested that the parental A allele has highly significant genetic effects in improving pig body development and carcass lean percent by increasing fiber diameter and the loin eye area, and decreasing the skin thicknes and fat percent.