遗传 ›› 2008, Vol. 30 ›› Issue (6): 755-759.doi: 10.3724/SP.J.1005.2008.00755

• 研究报告 • 上一篇    下一篇

SIM1基因第8外显子的SNP对猪背膘厚的影响

郭晓令; 陈哲; 赵晓枫; 徐宁迎   

  1. 浙江大学动物科学学院, 杭州 310029

  • 收稿日期:2007-10-27 修回日期:2008-01-20 出版日期:2008-06-10 发布日期:2008-06-10
  • 通讯作者: 徐宁迎

Effect of SNP in the exon 8 of SIM1 gene on backfat thickness in pigs

GUO Xiao-Ling;CHEN Zhe;ZHAO Xiao-Feng;XU Ning-Ying   

  1. College of Animal Science, Zhejiang University, Hangzhou 310029, China
  • Received:2007-10-27 Revised:2008-01-20 Online:2008-06-10 Published:2008-06-10
  • Contact: XU Ning-Ying

摘要:

采用PCR-SSCP方法检测了约克夏、杜洛克、皮特兰、长白猪、嘉兴黑猪和金华猪6个品种共169头猪的SIM1基因外显子8的SNP及其基因型频率。结果共发现CC、CT、TT 3种基因型, 其基因型频率在国内外猪品种之间具有较大差异。其中, 国内猪种嘉兴黑猪和金华猪只存在TT基因型, 而国外猪种约克夏、杜洛克、皮特兰、长白猪则都存在3种基因型。用最小二乘法分析SNP对长白猪、约克夏猪和杜洛克猪的背膘厚的效应的结果表明, 纯合基因型个体的背膘厚大于杂合基因型个体。SIM1基因型对国外猪种背膘厚有显著效应(P< 0.05), 并且不同部位效应不同。

关键词: 背膘厚, 猪, SNP, SIM1基因

Abstract:

SNP in exon 8 of the Single-minded 1 (SIM1) gene was detected using PCR-SSCP in 169 pigs of 6 pig breeds including Yorkshire, Duroc, Pietrain, Landrace, Jiaxinghei and Jinhua. The results showed three genotypes (CC, CT, TT) were found and their frequencies between domestic and foreign breeds were different. Only one genotype TT was found in Jiaxinghei and Jinhua pigs, but three genotypes were detected in each foreign breed. The effects of this SNP on backfat thickness in Landrace, Yorkshire and Duroc were estimated using least square method. The homozygote pigs had thicker backfat than the heterozygote ones. The significant effect of various genotypes was found in foreign breeds (P < 0.05). SIM1 had different effects on backfat thickness of different points.