遗传 ›› 2008, Vol. 30 ›› Issue (10): 1326-1332.doi: 10.3724/SP.J.1005.2008.01326

• 研究报告 • 上一篇    下一篇

应用生物信息学寻找山羊新的microRNA分子及其实验验证

陈海漩1, 2; 严忠海1, 2; 龙健儿1, 2; 颜景斌1, 2; 黄英1, 2

  

  1. 1. 上海交通大学医学遗传研究所, 上海 200040;
    2. 卫生部医学胚胎分子生物学重点实验室, 上海市胚胎与生殖工程重点实验室, 上海 200040

  • 收稿日期:2008-03-02 修回日期:2008-04-02 出版日期:2008-10-10 发布日期:2008-10-10
  • 通讯作者: 黄英

Computational and experimental identification of novel microRNAs in goat

CHEN Hai-Xuan1, 2; YAN Zhong-Hai1, 2; LONG Jian-Er1, 2; YAN Jing-Bin1, 2; HUANG Ying1, 2

  

  1. 1. Shanghai Institute of Medical Genetics, Shanghai Jiao Tong University, Shanghai 200040, China;
    2. Key Lab of Medical Embryo Molecular Biology, the Ministry of Health; Shanghai Laboratory of Embryo and Reproduction Engineering, Shanghai 200040, China
  • Received:2008-03-02 Revised:2008-04-02 Online:2008-10-10 Published:2008-10-10
  • Contact: HUANG Ying

摘要:

摘要: microRNA(miRNA)是一类长约22个碱基的非编码RNA分子, 在转录后水平调节基因的表达及其在细胞的增殖、分化、凋亡等过程中起着重要的调控作用。根据miRNA分子具有一定的保守性, 文章将人、小鼠、牛、猪和狗5种哺乳动物已知的miRNA分子与NCBI公布的与山羊具有极高同源性的绵羊基因组序列对比, 获得11条miRNA候选分子, 然后通过逆转录聚合酶链反应(RT-PCR)验证, 发现在山羊脑组织中这11条分子均有表达, 肝脏组织中有5条分子表达, 初步确定为山羊新的miRNA分子, 为寻找山羊miRNA提供了新的 思路。

关键词: miRNA, 山羊, 生物信息学

Abstract:

Abstract: microRNA (miRNA) is a class of non-coding small RNA molecules with roughly 22 nucleotides in length, regu-lating gene expression on post-transcriptional level and playing an important role in cell proliferation, differentiation and apoptosis process. Based on the conservation of miRNAs sequence, we compared the known miRNAs among five mam-mals, i.e., human, mouse, cattle, pig and dog with the sequence of sheep genome that is highly homologous to goat genome, published on the NCBI, and 11 candidate miRNAs were eventually obtained. RT-PCR analysis showed the expression of the 11 miRNAs in brain and 5 in liver, indicating that they might be novel miRNAs. The methodology provides an alternative approach to the exploration of new miRNAs in goat.