防御素的生物学特性及其抗病基因工程

doi:10.3724/SP.J.1005.2011.00512

遗传 ›› 2011, Vol. 33 ›› Issue (5): 512-519.doi: 10.3724/SP.J.1005.2011.00512

防御素的生物学特性及其抗病基因工程

付蓝宝^{1, 2}, 于嘉林¹, 刘伟华²

1. 中国农业大学农业生物技术国家重点实验室, 北京 100193 2. 中国农业科学院作物科学研究所, 农作物基因资源与基因改良国家重大科学工程, 北京 100081

收稿日期:2010-11-01 修回日期:2011-01-27 出版日期:2011-05-20 发布日期:2011-05-25
通讯作者: 刘伟华 E-mail:liuwh@caas.net.cn
基金资助:
转基因生物新品种培育重大专项(编号：2008ZX08002-001)资助

Biological characteristics of defensin and its disease-resistance genetic engineering

FU Lan-Bao^{1, 2}, YU Jia-Lin¹, LIU Wei-Hua²

1. The State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing 100193, China 2. National Key Facilities for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2010-11-01 Revised:2011-01-27 Online:2011-05-20 Published:2011-05-25
Contact: LIU Wei-Hua E-mail:liuwh@caas.net.cn

摘要/Abstract

摘要： 防御素是一种富含半胱氨酸的小分子多肽, 对细菌等微生物具有广谱抗性, 且作用机制特殊。迄今为止, 国内外在防御素方面进行了大量的研究, 已经从各类生物体中分离出不同种类的防御素, 并在基因工程和医药领域呈现广泛的应用前景。文章对防御素的分类、生物学特性, 包括哺乳动物α-、β-、θ-防御素、昆虫以及植物防御素的分子结构及抗菌活性进行了综述, 阐述了防御素的膜作用及与细胞内复合物结合的作用机制。总结和归纳了防御素基因的分离、表达研究进展及动、植物防御素基因在抗病基因工程领域的应用, 并对防御素在未来的生物制药和植物抗病基因工程方面的应用前景进行了展望。

关键词: 防御素, 抗病, 基因工程

Abstract: Defensin is a kind of cysteine-rich small peptide, which has a broad spectrum of resistance to bacteria with a special resistance mechanism. So far, a large number of studies on defensins have been reported, and the different types of defensins have been isolated from various organisms. A broad prospect of application on defensins has been displayed both in genetic engineering and medicine field. This article reviewed the classification and the biological characteristics of defensins, including mammalian α-, β-, θ-defensins, insect defensins, and plant defensins. The molecular structures, antibacterial activities, and antibacterial mechanisms of these definsins were summarized. The two mechanisms of de-fensin, including independent membrane mechanism and targeting of intracellular compounds by defensins, are ex-pounded. This paper also summarized the researches on isolation and expression of defensin genes and disease resistance genetic engineering of mammal and plant defensins. A prospect of the future applications of defensin both in biophar-maceutical sciences and plant disease resistance genetic engineering was discussed.

Key words: defensin, disease resistance, genetic engineering

付蓝宝，于嘉林，刘伟华. 防御素的生物学特性及其抗病基因工程[J]. 遗传, 2011, 33(5): 512-519.

FU La-Bao, XU Jia-Lin, LIU Wei-Hua. Biological characteristics of defensin and its disease-resistance genetic engineering[J]. HEREDITAS, 2011, 33(5): 512-519.

参考文献

[1] Crovella S, Antcheva N, Zelezetsky I, Boniotto M, Pacor S, Verga Falzacappa MV, Tossi A. Primate β-defensins-structure, function and evolution. Curr Protein Pept Sci, 2005, 6(1): 7-21.
[2] 傅荣昭, 李文彬, 孙勇如. 防御素的研究进展. 农业生物技术学报, 1996, 4(12): 348-353.
[3] Kim C, Kaufmann SHE. Defensin: a multifunctional molecule lives up to its versatile name. Trends Microbiol, 2006, 14(10): 428-431.
[4] Diamond G, Kaiser V, Rhodes J, Russell JP, Bevins CL. Transcriptional regulation of β-defensin gene expression in trachel epithelial cells. Infect Immun, 2000, 68(1): 113-119.
[5] Selsted ME, Miller CW, Novotny MJ, Morris WL, Koeffler HP. Molecular analysis of myeloperoxidase deficiency shows heterogeneous patterns of the complete deficiency state manifested at the genomic, mRNA, and protein levels. Blood, 1993, 82(4): 1317-1322.
[6] 王立刚, 王立贤, 程笃学. 动物防御素研究进展. 中国畜牧兽医, 2007, 34(3): 85-89.
[7] 余兴邦, 郭锁链, 乌翠兰, 李大竣, 呼格吉勒图, 鄂优. 防御素研究进展. 动物医学进展, 2006, 27(8): 47-51.
[8] Ashitani J, Nakazato M, Mukae H, Taniguchi H, Date Y, Matsukura S. Recombinant granulocyte colony-stimulating factor induces production of human neutrophil peptides in lung cancer patients with neutropenia. Regul Pept, 2000, 95(1-3): 87-92.
[9] 龙晶, 杜立新. 哺乳动物防御素研究进展. 中国畜牧兽医, 2007, 34(8): 137-140.
[10] Selsted ME, Ouellette AJ. Mammalian defensins in the antimicrobial immune response. Nat Immunol, 2005, 6(6): 551-557.
[11] Yount NY, Wang MS, Yuan J, Banaiee N, Ouellette AJ, Selsted ME. Rat neutrophil defensins. Precursor structures and expression during neutrophilic myelopoiesis. J Immunol, 1995, 155(9): 4476-4484.
[12] Torres AM, Kuchel PW. The β-defensin-fold family of polypeptides. Toxicon, 2004, 44(6): 581-588.
[13] Lehrer RI. Multispecific myeloid defensins. Curr Opin Hematol, 2007, 14(1): 16-21.
[14] Ericksen B, Wu ZB, Lu WY, Lehrer RI. Antibacterial activity and specificity of the six human α-defensins. Antimicrob Agents Chemother, 2005, 49(1): 269-275.
[15] Zou GZ, de Leeuw E, Li C, Pazgier M, Li CQ, Zeng PY, Lu WY, Lubkowski J, Lu WY. Toward understanding the cationicity of defensins: Arg and Lys versus their noncoded analogs. J Biol Chem, 2007, 282(27): 19653-19665.
[16] de Leeuw E, Burks SR, Li XQ, Kao JPY, Lu WY. Structure-dependent functional properties of human defensin 5. FEBS Lett, 2007, 581(3): 515-520.
[17] Maemoto A, Qu XQ, Rosengren KJ, Tanabe H, Henschen-Edman A, Craik DJ, Ouellette AJ. Functional analysis of the α-defensin disulfide array in mouse cryptdin-4. J Biol Chem, 2004, 279(4): 44188-44196.
[18] Wei G, de Leeuw E, Pazgier M, Yuan WR, Zou GZ, Wang JF, Ericksen B, Lu WY, Lehrer RI, Lu WY. Through the looking glass, mechanistic insights from enantiomeric human defensins. J Biol Chem, 2009, 284(42): 29180-29192.
[19] Breukink E, de Kruijff B. Lipid II as a target for antibiotics. Nat Rev Drug Discov, 2006, 5(4): 321-332.
[20] de Leeuw E, Li CQ, Zeng PY, Li C, Diepeveen-de Buin M, Lu WY, Breukink E, Lu WY. Functional interaction of human neutrophil peptide-1 with the cell wall precursor lipid II. FEBS Lett, 2010, 584(8): 1543-1548.
[21] Li DH, Li JZ. Antifungal activity of a recombinant defensin CADEF1 produced by Escherichia coli. World J Microbiol Biotechnol, 2009, 25(11): 1911-1918.
[22] Murphy CJ, Foster BA, Mannis MJ, Selsted ME, Reid TW. Defensins are mitogenic for epithelial cells and fibroblasts. J Cell Physiol, 1993, 155(2): 408-413.
[23] Lichtenstein A, Ganz T, Selsted ME, Lehrer RI. In vitro tumor cell cytolysis mediated by peptide defensins of human and rabbit granulocytes. Blood, 1986, 68(6): 1407-1410.
[24] Ganz T, Selsted ME, Szklarek D, Harwig SS, Daher K, Bainton DF, Lehrer RI. Defensins, natural peptide antibiotics of human neutrophils. J Clin Invest, 198

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防御素的生物学特性及其抗病基因工程

Biological characteristics of defensin and its disease-resistance genetic engineering

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