遗传 ›› 1995, Vol. 17 ›› Issue (1): 4-6.
毛新1 ;李虹2 ;陈文元3 MAO Xin1;LI Hong2;CHEN Wen-Yuan3
摘要： 用限制性内切酶AluⅠ、缓冲液或空白处理人染色体标本,发现AluⅠ及缓冲液均能诱导出类C和G带,其中缓冲液诱导的类C带频率虽低于酶原液,但明显高于空白对照; 而G带的诱导率无明显差异。此外,用限制性内切酶H ae Ⅲ和 HinfⅠ及其缓冲液处理中国小型白兔外周血染色体标本,也诱导出了相应的类C和G带。
Abstract:C-and G-bands were induced in human chromosome by both AluI and its buffer after treating the samples with AluI,buffer and blank control.The frequence of C-band induced by buffer was lower than that by AluI(P<0.05),but much higher than that by blank control(P<0.001).There was no significant difference among the frequencies of G-band induced by AluI,buffer and blank control(P>0.05).Also HaeIII,and their buffers were capable of inducing C-and G-bands on chromosome of China small-sized domestic rabbits.
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