遗传 ›› 1998, Vol. 20 ›› Issue (2): 27-30.

• 论文 • 上一篇    下一篇

水稻一多拷贝微卫星DNA多态性分析

杨官品1; M.A.Saghai Maroof2; 张启发3; 秦昌华1; 罗忠训1 YANG Guan-pin1;M.A.Saghai Maroof2;ZHANG Qi-fa3;QIN Chang-hua1;LUO Zhong-xun1   

  1. 1.湖北大学生命科学系, 武汉 430062 2.CSES Department,Virginia Tech,VA24061,USA 3.华中农业大学作物遗传改良国家重点实验室,武汉 430070 1.Department of Life Science,Hubei University,Wuhan 430062 2.CSES Department,Virginia Tech,VA24061,USA 3.The State Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070
  • 收稿日期:1900-01-01 出版日期:1998-04-10 发布日期:1998-04-10

Genetic Diversity of Rice Detected by a Multiple Copy Microsatellite DNA Marker

  • Received:1900-01-01 Online:1998-04-10 Published:1998-04-10

摘要: 用一个多拷贝微卫星 DNA标记分析了238份栽培水稻的遗传多样性及遗传多样性从农家品种到现代栽培品种的动态变化。共检测出16种长度变异类型和32种表现型,但长度变异类型数、表现型数和表现型多样性水平在农家品种和现代栽培品种间没有显著差别。该标记在遗传资源评价和DNA指纹图谱建设中有重要的应用价值,也是研究环境或人工对多拷贝基因选择作用的一个良好模型。
Abstract:The genetic diversity of 238 cultivated rice and its difference between landraces and modern cultivars have been assayed using a multiple copy microsatellite DNA marker.Although 16 length variants and 32 phenotypes were found in all accessions,no significant differences in number of length variants,number of phenotypes and phenotypic diversity were detected between landraces and modern cultivars.This marker is of great value in evaluating genetic germplasm and constructing DNA fingerprints of rice and provides an elite model for tracing effectiveness of natural and artificial selection on multiple copy genes.

关键词: Oryza sativa L.Microsatellite DNA marker, Genetic diversity, 遗传多样性
Key words,
微卫星DNA标记, 水稻, 转化, Ac转坐因子, 单倍体烟草, 转座因子标签法

Key words: Ac tivator Transformation Haploid tobacco Transposon tagging