遗传 ›› 1999, Vol. 21 ›› Issue (6): 23-27.

• 论文 • 上一篇    下一篇

毛冠鹿B染色体多态及遗传机制探讨

束峰珏;张锡然;曹祥荣;李朝军 SHU Fengjue; ZHANG Xi-ran; CAO Xiang-rong; LI CHao-jun   

  1. 南京师范大学生物系,江苏南京210097 Department of Biology,Nanjing Normal University,Nanjing210097,China
  • 收稿日期:1900-01-01 出版日期:1999-12-10 发布日期:1999-12-10

Studies of the Polymorphism of Elaphodus cephalophus B Chromosomes and Its Transmit Mechanism

  • Received:1900-01-01 Online:1999-12-10 Published:1999-12-10

摘要: 采用原代培养和传代培养法, 对1头毛冠鹿的胚肺组织细胞进行观察,发现一种新的核型,其二倍体染色体数目为2n=48,核型公式为2M+2ST+42T+XX,出现1对大的末端着丝粒染色体,C-带显示该染色体与已报道的相关染色体同源,首次提出毛冠鹿B染色体多态,对其传递机制进行了探讨。
Abstract: Primary culture and subculture were adopted using lung cells of a female Elaphodus cephalophus' embryo. A new karyotype and a pair of big telocentric chromosomes were found. The diploid has 48 chromosomes, and the karyotype formula is 2M+2ST+42T+XX. C-banding analysis shows that these chromosomes are homologous with those reported relevant chromosomes. This article is the first to report the polymorphism of Elophodus cephalophus B-chromosomes and studies it's transmitted mechanism.

关键词: 原代培养, 传代培养, 核型, B染色体多态
Key words,
C-带, 胚胎发育, 家蚕, 蛋白质序列分析, 蛋白质图谱分析, 基因表达

Key words: Bombyx mori, Embryonic development, Amino acid sequencing, Protein 2D-PAGE pattern, Gene expression