关键词: 马铃薯蛋白酶抑制剂Ⅱ, 遗传, 粘虫, 活性

Abstract: The inheritance of rice lines transformed by protease inhibitor Ⅱ gene under control of different promoters was investigated by analysis of hygromycin resistance , PCR and Southern blot. For segregation patterns of foreign gene, 68.4% of the transgenic rice plants were conformed to a Mendelian ratio and in which the rate of transgenic plants with single copy was 63.6%. Quantitative analysis of PinⅡ protein expressed in transgenic rice plants showed that PinⅡ protein in fresh leaves was 160μg/g for Act- PinⅡ-2x, 176μg/g for Ubi- PinⅡ-2x, and 104μg/g for PIN5′- PinⅡ-4x separately while in control rice plants was only 20μg/g. The inhibitory activity against tryspin of PinⅡ gene driven by ActI and Ubi promoter reached 37.7% and 43.1% , much higher than that driven by PIN5’(29.2%). Bioassay for insect resistance to armyworm (Pseudaletia separata Walker) revealed that transgenic plants had increased their resistance to the pest but there was not significantly different from controls, and also there was no correlation between insect resistance to armyworm and quantity of PinⅡ protein as well as promoters in transgenic rice.