关键词: 人工雌核发育, 大黄鱼, 诱导, 微卫星分析

Abstract: Two group of gynogenesis Pseudosciaena crocea was induced by cool-shock method. The 24 fries and its parents of gynogenesis family G1 and family G2 were PCR amplified using 6 couple of microsatellite marker primers （C7、C13、C27、C44、D19、F27） and analyzed by microsatellite marker. The results showed that the gynogenesis diploid by cool-shock method gained the hatching ratio at 35.3%, the survival ratio of 45 days at 9.9%. 4 couple of primers can amplified out clear different parents bands. The results of primer C44 and F27 showed no male gene appearing in G1. They were all gynogenesis. But the amplified results of primer C27 and D19 showed appearing the male genes in 3 individuals of G2. They were filial generations normally. The gene purified ratio of two family posterities are 93.8% and 72.9%. the average is 83.3%. The studies made know that Gynogenesis was a effective method to promote gene purification. microsatellite marker technique is an effective method of gynogenesis verification and genetic analysis.