鲤鱼(Cyprinus carpio L.)头长、眼径、眼间距QTL的定位

doi:10.3724/SP.J.1005.2009.00508

遗传 ›› 2009, Vol. 31 ›› Issue (5): 508-514.doi: 10.3724/SP.J.1005.2009.00508

鲤鱼(Cyprinus carpio L.)头长、眼径、眼间距QTL的定位

刘继红^{1, 2};张研³;常玉梅^{1, 4};梁利群¹;鲁翠云¹;张晓峰¹;徐美佳^{1, 2};孙效文¹

1. 黑龙江水产研究所北方鱼类生物工程育种重点开放实验室, 哈尔滨 150070;
2. 大连水产学院生命科学与技术学院, 大连 116023;
3. 中国水产科学研究院水产生物应用基因组研究中心, 北京 100141;
4. 中山大学生命科学学院, 广州 510275

收稿日期:2008-07-26 修回日期:2009-01-05 出版日期:2009-05-10 发布日期:2009-05-10
通讯作者: 孙效文

Mapping QTLs related to head length, eye diameter and eye cross of common carp (Cyprinus carpio L.)

LIU Ji–Hong^{1, 2};ZHANG Yan^{1, 3};CHANG Yu-Mei^{1, 4};LIANG Li-Qun¹;LU Cui-Yun¹; ZHANG Xiao-Feng¹;XU Mei-Jia^{1, 2};SUN Xiao-Wen¹

1. Heilongjiang Fisheries Institute, Chinese Academy of Fisheries Sciences, Harbin 150070, China;
2. College of Aqu-life Science and Technology, Dalian Fisheries University, Dalian 116023, China;
3. The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China;
4. College of Life Sciences, Sun-Yat Sen University, Guangzhou 510275, China

Received:2008-07-26 Revised:2009-01-05 Online:2009-05-10 Published:2009-05-10
Contact: SUN Xiao-Wen

摘要/Abstract

摘要： 用265个AFLP标记、127个微卫星分子标记、37个EST-SSR标记和16个RAPD标记对大头鲤/荷包红鲤抗寒品系的F2雌核发育群体44个个体进行基因型检测, 构建鲤鱼遗传连锁图谱。利用软件WinQTLCart2.5采用复合区间作图法对头长、眼径、眼间距3个性状进行了QTL分析。结果显示, 共检测到5个与头长性状相关的QTL, 分别定位于鲤鱼连锁图谱的LG2(qHS-2-1)、LG3 (qHS-3-1)、LG40(qHS-40-1)和LG4 (qHS-4-2和qHS-4-3) 上。其中qHS-40-1拥有最大的 LOD值, 为7.94, 可解释的表型变异为 34.29%。qHS-4-2的LOD值最小, 为5.03, 可解释的表型变异为11.52%。5个与头长性状相关的 QTL 加性效应值均为负值。检测到两个与眼径性状相关的QTL, 分别定位于鲤鱼连锁图谱的LG39连锁群(qED-39-1)和LG40连锁群(qED-40-1)上, 其中qED-40-1的LOD值比较大, 为2.76, 其加性效应值为负值, 可以解释5.62%的表型变异; qED-39-1的LOD值为2.72, 加性效应值为正值, 可以解释9.77%的表型变异。检测到两个与眼间距性状相关的QTL, 分别定位到鲤鱼连锁图谱的LG20连锁群(qEC-20-1)和LG28 连锁群(qEC-28-1)上。其中qEC-20-1的LOD值比较大, 为3.77, 加性效应值为正值, 可以解释8.29%的表型变异; qEC-28-1的LOD值为2.79, 对应的加性效应值为负值, 可以解释8.88%的表型变异

关键词: 眼径, 眼间距, QTL, 头长, 鲤鱼

Abstract: A common carp gynogenetic line including 44 individuals derived from the cross Barbless carp × Hebao-cold tolerance red carp was used to construct a linkage map using 445 markers (265 AFLP markers, 127 SSR markers, 37 EST-SSR markers and 16 RAPD markers). Quantitative traits loci (QTLs) associated with head length, eye diameter, and eye cross were identified by composite interval mapping of the software WinQTLCart2.5. Five QTLs were identified for head length on the linkage group of LG2, LG3, LG40, and LG4, which explained 12.39% to 34.29% of the total variation of the head length. All of their additive effects were negative. Two QTLs were associated with eye diameter on the linkage groups of LG39 (qED-39-1) and LG40 (qED-40-1), which explained 9.77% and 5.62% of the total variation of the eye di-ameter, respectively. The additive effect of qED-39-1 was positive and that of qED-40-1 was negative. Two QTLs were responsible for eye cross on the linkage group of LG28 and LG20, explaining 8.88% and 8.29% of the total variation of the eye cross, respectively. The additive effect of qEC-28-1 was negative and that of qEC-20-1 was positive.

刘继红，张研，常玉梅，梁利群，鲁翠云，张晓峰，徐美佳，孙效文 . 鲤鱼(Cyprinus carpio L.)头长、眼径、眼间距QTL的定位[J]. 遗传, 2009, 31(5): 508-514.

LIU Ji–Hong, ZHANG Yan, CHANG Yu-Mei, LIANG Li-Qun, LU Cui-Yun, ZHANG Xiao-Feng, XU Mei-Jia, SUN Xiao-Wen. Mapping QTLs related to head length, eye diameter and eye cross of common carp (Cyprinus carpio L.)[J]. HEREDITAS, 2009, 31(5): 508-514.

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鲤鱼(Cyprinus carpio L.)头长、眼径、眼间距QTL的定位

Mapping QTLs related to head length, eye diameter and eye cross of common carp (Cyprinus carpio L.)

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