遗传 ›› 2010, Vol. 32 ›› Issue (3): 219-228.doi: 10.3724/SP.J.1005.2010.00219

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ChIP技术及其在基因组水平上分析DNA与蛋白质相互作用

李敏俐1,王薇1,陆祖宏1,2   

  1. 1. 东南大学生物电子学国家重点实验室, 南京 210096;
     2. 东南大学儿童发展与学习科学教育部重点实验室, 南京 210096
  • 收稿日期:2009-06-23 修回日期:2009-08-27 出版日期:2010-03-20 发布日期:2010-03-18
  • 通讯作者: 陆祖宏 E-mail:zhlu@seu.edu.cn
  • 基金资助:

    国家自然科学基金项目(编号:30871393)资助

Genomic analysis of DNA-protein interaction by Chromatin immunoprecipitation

LI Min-Li1, WANG Wei1, LU Zu-Hong1,2   

  1. 1. The State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China; 
    2. Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing 210096, China
  • Received:2009-06-23 Revised:2009-08-27 Online:2010-03-20 Published:2010-03-18
  • Contact: LU Zu-Hong E-mail:zhlu@seu.edu.cn

摘要:

染色质免疫沉淀(Chromatin immunoprecipitaion, ChIP)技术是分析细胞内生理状态下DNA结合蛋白与基因组DNA相互作用的技术。ChIP与高密度芯片(ChIP-chip)或高通量测序(ChIP-Seq)相结合能产生大量的研究数据, 在细胞的基因表达调控网络研究中发挥重要作用。文章主要介绍ChIP、ChIP-chip和ChIP-Seq的技术特点以及发展趋势, 重点讨论了ChIP-Seq数据分析方法及相关的应用实例。

关键词: 基因组, ChIP-Seq, ChIP-chip

Abstract:

Chromatin immunoprecipitation (ChIP) is an effective technique to analyze the interactions of DNA binding proteins with the genome in vivo. ChIP coupled with high density microarray (ChIP-chip) or high-throughput sequencing (ChIP-Seq) has generated large amount of data and expected to allow the development of a network describing the cellular transcriptional regulation. Here, we reviewed the ChIP, ChIP-chip, and ChIP-Seq techniques as well as their perspectives. Focus is given to data analysis of ChIP-Seq and the applications of ChIP-chip and ChIP-Seq.

Key words: genome, ChIP-Seq, ChIP-chip