猪PEG1基因多态性及其遗传印记

doi:10.3724/SP.J.1005.2011.00738

遗传 ›› 2011, Vol. 33 ›› Issue (7): 738-742.doi: 10.3724/SP.J.1005.2011.00738

猪PEG1基因多态性及其遗传印记

李莉¹, 贾同¹, 吴同山², 张守全¹

1. 华南农业大学动物科学学院, 广东省农业动物基因组学与分子育种重点实验室, 广州 510642 2. 东莞市畜牧科学研究所, 东莞 523086

收稿日期:2010-08-10 修回日期:2011-03-08 出版日期:2011-07-20 发布日期:2011-07-25
通讯作者: 张守全 E-mail:sqzhang@scau.edu.cn
基金资助:
国家重点基础研究发展规划(973计划)项目(编号：2010CB945001; 2011CB944202)资助

Studies on SNP and genomic imprinting of the PEG1 gene in swine

LI Li¹, JIA Tong¹, WU Tong-Shan², ZHANG Shou-Quan¹

1. Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agriculture University, Guangzhou 510642, China 2. Animal Science Institute of Dongguan, Dongguan 523086, China

Received:2010-08-10 Revised:2011-03-08 Online:2011-07-20 Published:2011-07-25

摘要/Abstract

摘要： PEG1基因影响动物胚胎生长及母性行为, 多数动物PEG1为父方表达的遗传印记特征, 但出生后猪的PEG1印记表达尚不清楚。因此, 文章选取长白、大白和蓝塘3个品种共166头纯种猪, 在猪的PEG1基因外显子12区域内寻找SNP, 采用PCR-SSCP方法对其多态性进行检测和基因频率分析; 取带有PEG1基因该位点SNP为杂合的仔猪3头, 对其胃、胸腺、胰、脾、肺、肌肉、肝、舌、肾、脑、膀胱、心脏等组织器官和胎盘的mRNA产物分别进行RT-PCR-SSCP分析, 结果表明: PEG1基因外显子12存在一个由G突变为A的单核苷酸多态性位点; PEG1的外显子12在3头仔猪的主要组织器官仅表达父亲来源的等位基因, 表明猪的PEG1基因呈母方印记、父方表达的遗传特征。

关键词: 猪, PEG1, 单核苷酸多态性(SNP), 遗传印记

Abstract: PEG1 affects animal embryo growth and maternal behavior. The paternal allele-specific expression of PEG1 was reported in most animal species, but the expressive pattern of PEG1 was not clear in piglets born. In order to investigate the imprinting pattern of PEG1 in pig, 166 samples were used to SNP of PEG1 from Landrace, Yorkshire, and Lantang breeds by PCR-SSCP. Single nucleotide polymorphism (SNP) of PEG1 in exon 12 was identfied. The genotype frequency and the expressive pattern were anslyzed in swine by RT-PCR-RFLP/SSCP. One SNP (a G→A transition) was identified in exon 12 of PEG1. Maternal expression of PEG1 exon 12 was observed in all major organs (stomach, thymus, pancreas, spleen, lung, muscle, liver, tongue, kidney, brain, bladder, and heart) and placenta of three heterozygous pigs. PEG1 was paternally expressed and maternally imprinted in swine.

Key words: swine, PEG1, SNP, genomic imprinting

李莉，贾同，吴同山，张守全. 猪PEG1基因多态性及其遗传印记[J]. 遗传, 2011, 33(7): 738-742.

LI Chi, GU Tong, TUN Tong-Shan, ZHANG Shou-Quan. Studies on SNP and genomic imprinting of the PEG1 gene in swine[J]. HEREDITAS, 2011, 33(7): 738-742.

参考文献

[1] Li T, Vu TH, Lee KO, Yang YW, Nguynen CV, Bui HQ, Zeng ZL, Nguyen BT, Hu JF, Murphy SK, Jirtle RL, Hoffman AR. An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa. Biol Chem, 2002, 277(16): 13518-13527.
[2] Beechey CV, Cattanach BM. Mouse imprinting. http://www.mgu.har.mrc.ac.uk. 2000.
[3] Matsumoto S, Katoh M, Oshimura M, Nakanishi H, Kodani M, Nakamoto M, Shigeoka Y, Shimizu E, Suda T, Okabe T, Watanabe A, Igishi T. Loss of imprinting of PEG1/MEST in lung cancer cell lines. Oncol Rep, 2004, 12(6): 1273-1278.
[4] Xu CC, Su LJ, Zhou QY, Li CC, Zhao SH. Imprinting analysis of the porcine MEST gene in 75 and 90 day placentas and prenatal tissues. Acta Biochim Biophys Sin, 2007, 39(8): 633-639.
[5] Barlow DP, Stöger R, Herrmann BG, Saito K, Schweifer N. The mouse insulin-like growth factor type-2 receptor is imprinted and closely linked to the Tme locus. Nature, 1991, 349(6304): 84-87.
[6] Kalscheuer VM, Mariman EC, Schepens MT, Rehder H, Ropers HH. The insulin-like growth factor type-2 receptor gene is imprinted in the mouse but not in humans. Nat Genet, 1993, 5(1): 74-78.
[7] Nolan CM, Killian JK, Petitte JN, Jirtle RL. Imprint status of M6P/IGF2R and IGF2 in chickens. Dev Genes Evol, 2001, 211(4): 179-183.
[8] Pedersen IS, Dervan P, McGoldrick A, Harrison M, Ponchel F, Speirs V, Isaacs JD, Gorey T, McCann A. Promoter switch: a novel mechanism causing biallelic PEG1/MEST expression in invasive breast cancer. Hum Mol Genet, 2002, 11(12): 1449-1453.
[9] Riesewijk AM, Blagitko N, Schinzel AA, Hu LD, Schulz U, Hamel BC, Ropers HH, Kalscheuer VM. Evidence against a major role of PEG1/MEST in Silver-Russell syndrome. Eur J Hum Genet, 1998, 6(2): 114-120.
[10] Lefebvre L, Viville S, Barton SC, Ishino F, Surani MA. Genomic structure and parent-of-origin-specific methylation of Peg1. Hum Mol Genet, 1997, 6(11): 1907-1915.
[11] Nishita Y, Sado T, Yoshida I, Takagi N. Effect of CpG methylation on expression of the mouse imprinted gene Mest. Gene, 1999, 226(2): 199-209.

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猪PEG1基因多态性及其遗传印记

Studies on SNP and genomic imprinting of the PEG1 gene in swine

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