遗传 ›› 2007, Vol. 29 ›› Issue (10): 1230-1230―1236.

• 研究报告 • 上一篇    下一篇

湖羊6号染色体微卫星标记多样性与产羔数的关系

管峰1, 石国庆2, 艾君涛3, 刘守仁2, 杨利国4   

  1. 1. 中国计量学院生命科学学院, 杭州 310018;
    2. 新疆兵团绵羊繁育生物技术重点实验室, 石河子 832000;
    3. 山东省威海出入境检验检疫局, 威海 264200;
    4. 华中农业大学农业动物遗传育种与繁殖教育部重点试验室, 武汉 430070

  • 收稿日期:2007-03-07 修回日期:2007-05-28 出版日期:2007-10-10 发布日期:2007-10-10
  • 通讯作者: 杨利国

Relationship between genetic diversity of chromosome 6 determined by microsatellite markers and litter size in Hu sheep

GUAN Feng1, SHI Guo-Qing2, AI Jun-Tao3, LIU Shou-Ren2, YANG Li-Guo4

  

  1. 1. College of Life Sciences, China Jiliang University, Hangzhou 310018, China;
    2. The Key Sheep Breeding and Reproduction Biotechnology Laboratory of Xinjiang Production and Construction Group, Shihezi 832000, China;
    3. Shandong Weihai Entry-Exit Inspection and Quarantine Bureau, Weihai 264200, China;
    4. Ministry of Education Key Laboratory for Agriculture Animal Heredity and Breeding, Huazhong Agriculture University, Wuhan 430070, China
  • Received:2007-03-07 Revised:2007-05-28 Online:2007-10-10 Published:2007-10-10
  • Contact: YANG Li-Guo

摘要:

选择位于绵羊6号染色体上FecB基因紧密连锁的6个微卫星标记, 即LSCV043、BMS2508、GC101、300U、Bulge5和471U, 分析其在湖羊中的遗传多样性以及和产羔数的关系。结果表明, 6个微卫星位点均属多态性位点, 共检测到34个等位基因、53种基因型。LSCV043、BMS2508和300U属高度多态位点, 其多态信息含量分别为0.6674、0.6035和0.5615。对不同基因型群体的产羔率进行统计分析, LSCV043基因型为107 bp/123 bp所对应的总体产羔数明显高于110 bp/123 bp基因型群体所对应的产羔数(P<0.05); BMS2508基因型为154 bp/154 bp、154 bp/170 bp和154 bp/200 bp所对应的群体第一胎产羔数明显高于170 bp/170 bp所对应的产羔数(P<0.05), 基因型170 bp/170 bp的群体总体产羔数均低于该位点的其他基因型(P<0.05); 其他位点各基因型之间产羔数均无显著差异。

关键词: 湖羊, 微卫星, 产羔数, 遗传多样性

Abstract:

Genetic diversity and relationship with litter size were analyzed in Hu sheep using 6 microsatellite markers LSCV043, BMS2508, GC101, 300U, Bulge5, and 471U, which were closely linked to FecB gene. Thirty-four alleles and 53 genotypes were detected at 6 microsatellite loci, which were all polymorphic. Three markers LSCV043, BMS2508 and 300 U were highly polymorphic loci. The polymorphism information content (PIC) were 0.6674, 0.6035, and 0.5615, respectively. The total mean of litter size of genotype LSCV043 107 bp/123 bp was significantly higher than that of genotype 110 bp/123 bp. The litter size of the first lambing for genotypes BMS2508 154 bp/154 bp, 154 bp/170 bp and 154 bp/200 bp were significantly higher than that of genotype 170 bp/170 bp (P<0.05). Furthermore, total litter size for BMS2508 170 bp/170 bp was the lowest in all the genotypes within this locus (P<0.05). No significant difference was detected in other genotypes.